关键词: 美洲大蠊提取物, Ⅱ型单纯疱疹病毒, 抗病毒作用

Abstract:

Objective: To study the anti-HSV-2 effect of CII-3 extracted from Periplaneta Americana. Methods: Anti-HSV-2
activity of CII-3 extracted from Periplaneta Americana was observed and determined with MTT assay and cytopathic effect (CPE) assay
and the value of TC₅₀, IC₅₀ and TI in the extract was calculated. Female mice with genital herpes were used as animal models. 50 μL of
HSV- 2 was injected into mice vaginas once a day with 7 consecutive days. With acyclovir as the positive control, intragastric
administration was adopted as treatment for 10 days. The ELISA KIT was used to test the concentration of vascular tumor necrosis
factor-α (TNF-α), Interferon-γ (IFN-γ) and Interleukin-2 (IL-2); the immune organs were weighed to calculate the organ index.
Results: CII-3 extracted from Periplaneta Americana had anti-HSV-2 activity. The TC₅₀ value was 190.55 μg/mL, IC50 value was
28.71 μg/mL and TI value was 6.64 μg/mL. CII-3 had the function of increasing TNF-α, IFN-γ, IL-2 and could increase the weight
of the immune organs in mice. Conclusion: CII-3 extracted from Periplaneta Americana had the effect of anti-HSV-2 by boosting the
body's immunity.

Key words: Periplaneta Americana extract, HSV-2, antiviral effect