葡萄籽原花青素对缺血再灌注肝脏氧化损伤和细胞凋亡的影响

J4 ›› 2014, Vol. 13 ›› Issue (8): 11-14.

葡萄籽原花青素对缺血再灌注肝脏氧化损伤和细胞凋亡的影响

1.大理学院附属医院，云南大理 671000；2.大理学院基础医学院，云南大理 671000

收稿日期:2014-02-20 出版日期:2014-08-15 发布日期:2014-08-15
作者简介:刘仁贵，副主任医师，主要从事普外基础与临床工作研究.
基金资助:
云南省教育厅基金资助项目（0900158）

Effects of Grape Seed Proanthocyanidin Extract on Hepatic Ischemia Reperfusion
Oxidative Injury and Apoptosis

1.AffiliatedHospitalofDaliUniversity,Dali,Yunnan 671000,China;2.Pre-clinicalCollege,DaliUniversity,Dali,Yunnan 671000,China

Received:2014-02-20 Online:2014-08-15 Published:2014-08-15

摘要/Abstract

摘要：

目的：探讨葡萄籽原花青素对大鼠肝缺血再灌注损伤的抗氧化作用、凋亡相关蛋白Bcl-2和Bax表达的影响。方法：SD
雄性大鼠30只，复制肝缺血再灌注损伤模型。随机分为：假手术组；HIRI模型组；葡萄籽原花青素预处理（GSPE）组（160 mg/kg），每组10只。于再灌注后3 h检测肝组织谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）、丙二醛（MDA）含量及血清谷丙转氨酶（ALT）、谷草转氨酶（AST）的含量；光镜下比较各组肝组织形态学的变化；免疫组化法检测GSPE对凋亡相关蛋白Bcl-2，Bax表达的影响。结果：与HIRI模型组比较，GSPE预处理组血清ALT、AST水平明显降低（P＜0.05）；肝组织GSH-Px 、SOD 的活性明显升高（P＜0.05），而MDA含量下降（P＜0.05）。光镜下GSPE预处理组肝细胞损伤程度较模型组明显减轻；免疫组化结果显示，GSPE预处理组肝组织Bcl-2蛋白的表达量较模型组明显增多，而Bax蛋白的表达量则较模型组明显减少。结论：GSPE对缺血再灌注肝脏具有一定的抗氧化损伤和抗凋亡的作用。

关键词: 葡萄籽原花青素, 缺血再灌注, 肝脏, 氧化损伤, Bcl-2, Bax蛋白

Abstract:

Objective: To study the effects of grape seed proanthocyanidin extract（GSPE）on antioxidation of hepatic ischemia
reperfusion injury in rats, and the expression of apoptosis- related proteins Bcl- 2 and Bax. Methods: Thirty SD male rats were randomly divided into three groups with 10 rats each .i.e. sham operation group, HIRI model group and GSPE preconditioning group （160 mg/kg）. The content of GSH-Px, SOD, and MDA in hepatic tissues, and the content of ALT and AST in serum were assayed 3 hours later after reperfusion. The morphological variations of hepatic tissues in each group were compared by means of optical microscope. Effects of GSPE on Bcl-2 and Bax in hepatic tissues were tested by means of immunohistochemisty. Results: The content of ALT and AST in serum and MDA in hepatic tissues decreased significantly（P＜0.05）, and the content of GSH-Px and SOD in hepatic tissues increased remarkably（P＜0.05）in GSPE preconditioning group, compared with HIRI model; Observed by the optical
microscope, the degree of liver cell injury in GSPE preconditioning group was remarkably slighter than that of HIRI model group. Immunohistochemical results showed that the content of Bcl-2 increased and Bax decreased significantly in hepatic tissues, compared with that in HIRI model（P＜0.05）. Conclusion: GSPE had some effects on antioxidation and anti-apoptosis of hepatic ischemia reperfusion injury.

Key words: GSPE, ischemia reperfusion, liver, oxidative injuries, proteins Bcl-2 and Bax

中图分类号:

R657.3

刘仁贵, 张自华, 秦燕, 苏娟, 杨继武. 葡萄籽原花青素对缺血再灌注肝脏氧化损伤和细胞凋亡的影响[J]. J4, 2014, 13(8): 11-14.

LIU Ren-Gui, ZHANG Zi-Hua, QIN Yan, SU Juan, YANG Ji-Wu. Effects of Grape Seed Proanthocyanidin Extract on Hepatic Ischemia Reperfusion
Oxidative Injury and Apoptosis[J]. J4, 2014, 13(8): 11-14.

[1]	李秉昊, 刘蜻蜻. 超声造影对肝脏占位性病变的诊断价值[J]. , 2019, 4(8): 63-66.
[2]	和红阳, 苏子庭, 孙菱, 谭云波. Notch信号通路在缺血再灌注损伤后胆道周围血管丛再生中的作用[J]. , 2019, 4(10): 27-31.