J4 ›› 2015, Vol. 14 ›› Issue (12): 64-68.

• 生命科学 • 上一篇    下一篇

中药葛根微生物转化菌株的鉴定及转化特性的研究

  

  1. (1.大理大学公共卫生学院,云南大理671000;2.云南大学云南省微生物研究所,昆明650091)
  • 收稿日期:2015-05-14 出版日期:2015-12-15 发布日期:2015-12-15
  • 作者简介:宋艳秋,讲师,主要从事微生物学研究.

Study on Identification of the Microbial Strain for Transforming Puerariae Radix and Its Microbial
Characteristics

  1. (1. College of Public Health, Dali University, Dali, Yunnan 671000, China; 2. Yunnan Institute of Microbiology, Yunnan University,
    Kunming 650091, China)
  • Received:2015-05-14 Online:2015-12-15 Published:2015-12-15

摘要:

从中药葛根的微生物转化试验中,获得1株能够较好转化葛根有效成分的丝状真菌,编号为YM3207,通过对该菌的形
态特征观察及ITS序列分析,确定该菌为紫色红曲霉Monascus purpureus。经HPLC定量分析显示,转化后染料木素和葛根素含
量得到提高;同时,抑菌活性较转化前有一定程度的增强,抗菌谱变广。

关键词: 葛根, 微生物转化, 鉴定, 抑菌

Abstract:

A strain of filamentous fungi which was able to transform the active ingredient in Puerariae radix was obtained in microbial
transformation experiments and named YM3207. After observing and analyzing the morphology and ITS sequence of this fungi, it was
identified as Monascus purpureus. HPLC was used to analyze the change of transformation column. The content of Genistein and
Puerarin have been improved. At the same time, antibacterial activity enhanced and antimicrobial spectrum expanded as well.

Key words: Puerariae radix, microbial transformation, identification, antibacterial

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