肝癌经TAE术后肝细胞中PPAR-α的表达

J4 ›› 2016, Vol. 1 ›› Issue (10): 67-70.

肝癌经TAE术后肝细胞中PPAR-α的表达

（1.大理大学大理非直属附属医院，云南大理671000；2.大理大学第一附属医院，云南大理671000）

收稿日期:2014-06-09 修回日期:2016-05-03 出版日期:2016-10-15 发布日期:2016-10-15
作者简介:张靓，住院医师，主要从事放射介入研究.
基金资助:
国家自然科学基金资助项目（81241131）；云南省教育厅科学研究基金资助项目（2013J113）

PPAR-α Expression of Hepatocytes after TAE in Liver Cancer

（1. Non-Affiliated Hospital of Dali University in Dali, Dali, Yunnan 671000, China; 2. The First Affiliated Hospital of Dali University,
Dali, Yunnan 671000, China）

Received:2014-06-09 Revised:2016-05-03 Online:2016-10-15 Published:2016-10-15

摘要/Abstract

摘要：

目的：探讨PPAR-α在肝癌经肝动脉栓塞术（TAE）后肝功能异常中的作用机制。方法：建立新西兰大白兔VX2肝癌动
物模型26只，模型成功率为86%（24/28），将兔VX2肝癌动物模型随机分为栓塞组12只，造影组12只，TAE术后8 h各组兔同时
处死，取肿瘤旁取正常肝组织，制作石蜡切片，进行HE染色以及免疫组化，检测PPAR-α在肝组织中的表达。结果：栓塞组阳
性细胞数介于51%~85%，阳性评估为++/+++，造影组阳性细胞数介于10%~30%，阳性评估为+/++。统计学分析表明TAE术
后，癌旁肝组织中PPAR-α的表达明显高于造影组（P＜0.05）。结论：TAE后，PPAR-a在肿瘤周围肝细胞中的表达明显增加，
可能机制为癌旁正常肝组织缺血缺氧，从而导致PPAR-α高表达。

关键词: PPAR-a, 肝癌, 动脉栓塞术, 肝功能

Abstract:

Objective: To investigate the function mechanism of PPAR -α in liver cancer on abnormal liver function after
transcatheter arterial embolization（TAE）. Methods: VX2 hepatic cancer model in 26 New Zealand white rabbits model were
established with 86% successful rate（24/28）. VX2 hepatic cancer models in rabbits were randomly divided to embolization group
（12）, angiography group（12）. Rabbits of very group were sacrificed 8 hours after TAE. Liver tissues surrounded HCC were collected
to make paraffin section, and the expression of PPAR-α in hepatic tissue was detected after hematoxylin & eosin（HE）staining and
immunohistochemistry. Results: Positive cells in embolization group ranged from 51%~85% with a positive assessment ++ / +++;
positive cells in angiography group were between 10%~30% with a positive assessment of + / ++. Statistical analysis showed that the
PPAR-α expression in cancer adjacent hepatic tissue of embolization group was significantly higher than angiography group（P＜
0.05）. Conclusion: After TAE, PPAR-α expression in tumor adjacent hepatic tissue increased significantly. The possible reason of
high expression of PPAR-α might be hypoxia ischemia of cancer adjacent normal tissues.

Key words: PPAR-α, liver cancer, arterial embolization, liver function

中图分类号:

R735.7

张靓，王光明，唐艳隆，刘玲，杜伟. 肝癌经TAE术后肝细胞中PPAR-α的表达[J]. J4, 2016, 1(10): 67-70.

Zhang Liang,Wang Guangming, Tang Yanlong, Liu Ling, Du Wei. PPAR-α Expression of Hepatocytes after TAE in Liver Cancer[J]. J4, 2016, 1(10): 67-70.

参考文献

〔1〕张成佳，何仕诚，滕皋军，等. TACE治疗原发性肝癌对肝
功能影响的相关因素分析〔J〕. 东南大学学报（医学版），
2013，32（1）：18-22.
〔2〕PETERS J M，SHAH Y M，GONZALEZ F J. The role of
peroxisome proliferator-activated receptors in carcinogene?
sis and chemoprevention〔J〕. Nature Reviews Cancer，2012，
12（3）：181-195.
〔3〕王君东，胡锦波，杜伟. 改良方法建立兔VX2肝癌模型及
其MRI表现〔J〕.医学理论与实践，2013，26（6）：701-702.
〔4〕WANG R，CHEN X Z，ZHANG M G，et al. Incidence and
mortality of liver cancer in mainland China：changes in first
decade of 21st century〔J〕. Hepatogastroenterology，2015，
62（137）：118-121.
〔5〕DE BAERE T，ARAI Y，LENCIONI R，et al. Treatment of
Liver Tumors with Lipiodol TACE：Technical Recommenda?
tions from Experts Opinion〔J〕. Cardiovasc Intervent Radiol，
2015，39（3）：1-10.
〔6〕FIORE F，DEL PRETE M，FRANCO R，et al. Transarterial
embolization（TAE）is equally effective and slightly safer
than transarterial chemoembolization（TACE） to manage
liver metastases in neuroendocrine tumors〔J〕. Endocrine，
2014，47（1）：177-182.
〔7〕MASSMANN A，RODT T，MARQUARDT S，et al. Transar?
terial chemoembolization（TACE）for colorectal liver me?
tastases-- current status and critical review〔J〕. Langen?
becks Arch Surg，2015，400（6）：641-659.
〔8〕SCHNEEWEISS S，HORGER M，KETELSEN D，et al.
Complications after TACE in HCC - complications after
transarterial chemoembolization（TACE）in hepatocellular
carcinoma〕〔J〕.Rofo，2015，187（2）：79-82.
〔9〕WANG Y，XIONG X，GUO H，et al. ZnPP reduces autopha?
gy and induces apoptosis，thus aggravating liver ischemia/
reperfusion injury in vitro〔J〕. Int J Mol Med，2014，34（6）：
1555-1564.
〔10〕EL-SHITANY N A，EL-DESOKY K. Cromoglycate，not
ketotifen，ameliorated the injured effect of warm ischemia/
reperfusion in rat liver：role of mast cell degranulation，oxi?
dative stress，proinflammatory cytokine，and inducible ni?
tric oxide synthase〔J〕. Drug Des Devel Ther，2015，9：
5237-5246.
〔11〕FANG X，TAO D，SHEN J，et al. Neuroprotective effects
and dynamic expressions of MMP9 and TIMP1 associated
with atorvastatin pretreatment in ischemia- reperfusion
rats〔J〕. Neurosci Lett，2015，603：60-65.
〔12〕张靓，杜伟. NF-κB在肝癌介入治疗后癌旁肝细胞中的
表达〔J〕. 大理学院学报，2014，13（10）：42-46.
〔13〕EMANUELA E，RINALDI B，MAZZON E，et al. Anti-in?
flammatory effect of simvastatin in an experimental model
of spinal cord trauma：involvement of PPAR-a〔J〕. Jour?
nal of Neuroinflammation，2012，9（1）：81.
〔14〕PANIGRAHY D，KAIPAINEN A，HUANG S，et al. PPA?
Ralpha agonist fenofibrate suppresses tumor growth
through direct and indirect angiogenesis inhibition〔J〕.
Proceedings of the National Academy of Sciences of the
united States of America，2008，105（3）：985-990.
〔15〕KARUNA R，CAROL C A. Molecular mechanism of alco?
holic fatty liver〔J〕. Indian Journal of Pharmacology，2012，
44（3）：299-303.
〔16〕BECHMANN L P，VETTER D，ISHIDA J，et al. Posttranscriptional
activation of PPAR alpha by KLF6 in he?
patic steatosis〔J〕. Journal of Hepatology，2013，58（5）：
1000-1006.

[1]	吴春华,李星云,张悦，杨代仙,刘冬岩,杜伟. PPAR-α激动剂WY14643对兔VX2肝癌MMP-9表达的影响[J]. 大理大学学报, 2020, 5(2): 61-64.
[2]	杨改标，李倩，李璇，龚念婷，张胜梅. 血清AFP、B7-H4联合CP检测对原发性肝癌的诊断价值[J]. 大理大学学报, 2020, 5(10): 75-79.
[3]	李燕，邵明园，国超，杨强丽，曹昌娥，赖泳. 肝龙胶囊对猪血清诱导的肝纤维化大鼠的治疗作用[J]. , 2018, 3(10): 13-17.
[4]	李婷，乔婷婷，刘俊勇，沈琦，彭芳. 美洲大蠊提取物逆转Bel-7402/5-Fu多药耐药性实验研究[J]. , 2017, 2(2): 1-5.
[5]	张海洋, 冯慕华, 徐彤, 徐优, 余敏, 孙宝迪, 熊伟. 人核呼吸因子2基因真核表达载体的构建与鉴定[J]. J4, 2014, 13(2): 37-42.
[6]	张靓，杜伟. NF-κB在肝癌介入治疗后癌旁肝细胞中的表达[J]. J4, 2014, 13(10): 42-46.