›› 2017, Vol. 2 ›› Issue (8): 10-12.

• 药学 • 上一篇    下一篇

隔山消对豚鼠离体回肠收缩的促进作用及机制探讨

  

  1. (1. 大理大学基础医学院,云南大理671000;2. 楚雄医药高等专科学校,云南楚雄675000;3. 大理大学
    药学与化学学院,云南大理671000)
  • 收稿日期:2016-12-16 修回日期:2017-03-19 出版日期:2017-08-15 发布日期:2017-08-15
  • 作者简介:耿玲,实验师,主要从事免疫药理学研究.
  • 基金资助:

    大理大学青年教师科研基金资助项目(KYQN201309)

Exploring the Contractile Effect of Geranium strictipes R. Knuth on the Intestinal Muscle of the Isolated
Guinea-pig Ileum and Its Mechanism

  1. (1. Pre-clinical College, Dali University, Dali, Yunnan 671000, China; 2. Chuxiong Pharmaceutical College, Chuxiong,
    Yunnan 675000, China; 3. College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China)
  • Received:2016-12-16 Revised:2017-03-19 Online:2017-08-15 Published:2017-08-15

摘要:

目的:观察隔山消乙酸乙酯提取物对离体豚鼠回肠平滑肌收缩活动的影响,并初步探讨其体外作用机制。方法:制备
豚鼠离体回肠标本,观察平滑肌正常收缩曲线,分别记录单独使用不同浓度隔山消和使用阻断剂后隔山消对回肠平滑肌的收
缩作用。结果:不同浓度隔山消对离体豚鼠回肠平滑肌收缩促进作用明显,与空白对照组比较,20、40 mg/mL引起平滑肌收缩
的最大张力、频率和幅度差异具有统计学意义(P < 0.01),且其可有效对抗阿托品、氯苯那敏导致的豚鼠回肠舒张作用。结论:
隔山消对豚鼠离体回肠平滑肌的收缩活动有兴奋作用,其引起的收缩效应可能与M受体和H1受体有关。

关键词: 隔山消, 离体回肠, M受体, H1受体

Abstract:

Objective:To observe the effect of ethyl acetate extracts from Geranium strictipes R. Knuth on the isolated guinea-pig
ileum smooth muscle, and to explore the mechanism of its in vitro action. Methods:The normal contraction curve was observed after
the isolated ileum specimens were prepared. Then contraction in smooth muscle caused by Geranium strictipes R. Knuth and three
blockers was recorded. Results:The Geranium strictipes R. Knuth in different doses could accelerate the ileum smooth muscle
contraction of the guinea-pig. The maximum tension, frequency and amplitude induced by 20 and 40 mg/mL extracts had a statistical
difference(P < 0.01)compared with the normal group; and it could resist against ileum smooth muscle relaxation induced by Atropine
and Chlorobenzene. Conclusion:The Geranium strictipes R. Knuth has stimulating effects on ileum smooth muscle extraction of the
guinea-pig, and the contractile effect may be related to M receptor and H1 receptor.

Key words: Geranium strictipes R. Knuth, isolated ileum, M receptor, H1 receptor

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