大理大学学报 ›› 2019, Vol. 4 ›› Issue (2): 38-.

• 基础医学 • 上一篇    下一篇

利用TCGA数据集分析人MTERF3基因在子宫颈癌的表达及意义

梅雯,王唯斯,孙美涛,张态,柴蓓蓓,张若鹏,熊伟   

  1. (1. 大理大学基础医学院,云南大理671000;2. 大理大学公共卫生学院,云南大理671000;
    3. 大理大学第一附属医院,云南大理671000)
  • 收稿日期:2018-06-04 修回日期:2018-10-08 出版日期:2019-02-15 发布日期:2019-02-15
  • 通讯作者: 熊伟,教授,博士,E-mail:xwailp@163.com.
  • 作者简介:梅雯,硕士研究生,主要从事细胞分子生物学研究.
  • 基金资助:
    国家自然科学基金资助项目(81560458;31601155);云南省中青年学术技术带头人后备人才资助项目(2017HB077);
    云南省教育厅科学研究基金资助项目(2016ZDX01;2016ZDX05);大理大学大学生创新创业计划资助项目
    (S-CXCY-2017-8)

Expression of Human MTERF3 and Its Clinical Value in Cervical Cancer: An Analysis Based on the#br# TCGA Dataset

Mei Wen, Wang Weisi, Sun Meitao, Zhang Tai, Chai Beibei, Zhang Ruopeng, Xiong Wei   

  1. (1. Pre-clinical College, Dali University, Dali, Yunnan 671000, China; 2. College of Public Health, Dali University, Dali, Yunnan
    671000, China; 3. The First Affiliated Hospital of Dali University, Dali, Yunnan 671000, China)
  • Received:2018-06-04 Revised:2018-10-08 Online:2019-02-15 Published:2019-02-15

摘要: [摘要]目的:基于TCGA数据集探讨人MTERF3 基因mRNA在子宫颈癌的表达及其临床价值。方法:从美国癌症基因组图谱
(TCGA)中下载关于子宫颈癌患者MTERF3 mRNA二代测序数据及临床病理资料。基因表达谱动态分析(GEPIA)正常宫颈组
织和宫颈癌组织中MTERF3 mRNA表达差异。使用R 3.3.0软件分析MTERF3 mRNA表达量与临床病理参数的相关性;使用
Graphpad Prism 7.01软件对MTERF3 mRNA表达量和患者预后的关联进行Kaplan-Meier生存分析和Log-rank P 检验。结果:
与正常宫颈组织相比,子宫颈癌组织中MTERF3 mRNA呈现高表达。子宫颈癌患者MTERF3 mRNA表达量与年龄、T分期、N
分期、M分期差异均无统计学意义(P>0.05);而与AJCC临床分期、病理分级差异有统计学意义(P<0.05);Kaplan-Meier生存
分析显示,MTERF3 mRNA表达量与子宫颈癌患者的总体生存率和无疾病进展生存率差异均无统计学意义(Log-rank P>
0.05);单因素分析显示,T分期、N分期、M分期、临床分期均能影响患者的预后(P<0.05),年龄、病理分级、病理类型、MTERF3
mRNA表达与子宫颈癌患者预后无相关性(P>0.05);COX多因素回归分析显示,N分期是影响子宫颈癌患者预后的独立因素
(P<0.05)。结论:人MTERF3 mRNA表达量与子宫颈癌患者AJCC临床分期和病理分级有显著相关性,提示MTERF3 基因可能
参与子宫颈癌发展的病理进程;MTERF3 mRNA表达量与子宫颈癌患者的预后无显著相关性。

关键词: [关键词]TCGA数据集, MTERF3, 子宫颈癌

Abstract: 〔Abstract〕Objective: To explore the expression of human mitochondrial transcription termination factor 3(MTERF3)in cervical
cancer and its clinical value based on the TCGA dataset. Methods:The expression of MTERF3 mRNA and clinical information of
patients with cervical cancer was downloaded from TCGA database. Then the relationship between the expression of MTERF3 mRNA
and clinicopathological characteristics, and the prognostic value of MTERF3 were analyzed by R3.3.0 software; Graphpad Prism 7.01
software was applied to analyze the correlational relationship between the expression level of MTERF3 mRNA and prognosis of patients
by Kaplan-Meier and Log-rank test. Results: The expression level of MTERF3 mRNA was higher in the normal cervical tissues than in
cervical cancers. The expression of MTERF3 mRNA in patients with cervical cancer was not related to age, T stage, N stage and M stage,
but was related to AJCC clinical stage and tumor grade(P < 0.05). Moreover, there was no difference between the overall survival time
and the disease-free survival of cervical cancer patients and low MTERF3 expression or high MTERF3 expression(Log-rank P>0.05).
Univariate analysis showed that T stage, N stage, M stage and clinical stage affected the prognosis of cervical carcinoma(P < 0.05), but
was not related to age, grade, pathological type and the expression of MTERF3(P > 0.05). COX multivariate analysis showed that N
stage was an independent factor influencing patients' prognostic. Conclusion: The analysis of the TCGA dataset revealed that the
mRNA expression level of human MTERF3 is related to the clinical stage and tumor grade, but further analysis showed that there is no
significant correlation between the expression level of MTERF3 mRNA and the prognosis of patients with cervical cancer.

Key words: 〔Key words〕TCGA dataset, MTERF3 gene, cervical cancer