灯盏乙素对Chang Liver细胞中CYP2C9的影响

大理大学学报 ›› 2019, Vol. 4 ›› Issue (2): 5-.

灯盏乙素对Chang Liver细胞中CYP2C9的影响

丁未尧，陈丽平，赖泳

（大理大学药学与化学学院，云南大理671000）

收稿日期:2018-08-30 修回日期:2018-09-25 出版日期:2019-02-15 发布日期:2019-02-15
通讯作者: 赖泳，教授，E-mail：laiyong8879@163.com.
作者简介:丁未尧，硕士研究生，主要从事药理学研究.
基金资助:
国家自然科学基金资助项目（81360511）

Effect of Scutellarin on CYP2C9 in Chang Liver Cells

Ding Weiyao, Chen Liping, Lai Yong

（College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China）

Received:2018-08-30 Revised:2018-09-25 Online:2019-02-15 Published:2019-02-15

摘要/Abstract

摘要： ［摘要］目的：探讨灯盏乙素对Chang Liver细胞中CYP2C9表达的影响。方法：采用MTT（噻唑蓝）法检测灯盏乙素对Chang Liver
细胞增殖活力的影响，采用RT-PCR和Western-blot法分别检测细胞中CYP2C9 mRNA和蛋白的相对表达量。结果：灯盏乙素
对Chang Liver细胞增殖有抑制作用，呈浓度依赖性，半数抑制率（IC50）为1.87 mmol/L。RT-PCR结果表明，与DMSO（二甲基亚
砜）组比，药物浓度为5.0、10.0、20.0、40.0 μmol/L时，细胞CYP2C9 mRNA表达量显著下降（P<0.05）。Western-blot结果表明，与
DMSO组比较，药物浓度为20.0、40.0 μmol/L时细胞CYP2C9 蛋白表达显著减少（P<0.05）。结论：灯盏乙素对Chang Liver细胞
中CYP2C9 mRNA和蛋白的表达有不同程度的抑制作用。

关键词: ［关键字］灯盏乙素, CYP2C9, mRNA表达, 蛋白表达, Chang Liver细胞

Abstract: 〔Abstract〕Objective: To study the effect of scutellarin on the expression of CYP2C9 in Chang Liver cells. Methods: The effect of
scutellarin on proliferative activity of Chang Liver cells was detected by MTT assay. The relative expression of CYP2C9 mRNA and
protein was detected by RT- PCR and Western- blot respectively. Results: MTT results showed that scutellarin inhibited the
proliferation of Chang Liver cells in a concentration-dependent manner, and the half inhibitory rate（IC50）was 1.87 mmol/L. RT-PCR
results showed that the expression of CYP2C9 mRNA significantly decreased in 5.0, 10.0, 20.0, 40.0 μmol/L scutellarin groups（P<
0.05）, compared with DMSO group. Western- blot results showed that the expression of CYP2C9 protein in 20.0, 40.0 μmol/L
scutellarin groups significantly decreased（P<0.05）, compared with DMSO group. Conclusion: Scutellarin has different degree of
inhibitory action on the expression of CYP2C9 mRNA and protein in Chang Liver cells.

Key words: 〔Key words〕scutellarin, CYP2C9, mRNA expression, protein expression, Chang Liver cells

丁未尧，陈丽平，赖泳. 灯盏乙素对Chang Liver细胞中CYP2C9的影响[J]. 大理大学学报, 2019, 4(2): 5-.

Ding Weiyao, Chen Liping, Lai Yong. Effect of Scutellarin on CYP2C9 in Chang Liver Cells[J]. Journal of Dali University, 2019, 4(2): 5-.