关键词: font-family:宋体, ">羊角天麻；font-family:", "> font-family:宋体, ">提取；抑菌作用；font-family:", "> font-family:宋体, ">活性部位；稳定性

Abstract: Objective： To investigate the antibacterial effect and active parts of Dobinea delavayi. Methods： The water， petroleum ether， ethyl acetate， n-butanol， 30% ethanol precipitation， 60% ethanol precipitation， and 95% ethanol precipitation parts of D. delavayi were extracted， and the minimum inhibitory concentration of the above 7 parts against methicillin resistant Staphylococcus aureus（MRSA）， Staphylococcus aureus and Enterococcus faecalis were measured by agar plate double dilution method. The effects of temperature and ultraviolet on the antibacterial activity of different active parts of D. delavayi were also investigated. Results： The water part of D. delavayi had an inhibitory effect on MRSA， S. aureus， and E. faecalis. The 60% ethanol-precipitated part and 95% ethanol-precipitated part had an inhibitory effect on MRSA and S. aureus， with antibacterial zone diameters of （21.2±0.6）， （20.4±0.1）， （19.6±0.6）， （20.6±0.1）， （22.2±0.2）， （17.5±0.2）， （20.3±0.3） mm， respectively. The petroleum ether， ethyl acetate， and n-butanol parts had no antibacterial activity. In addition， the water part, the 60% ethanol-precipitated part, and the 90% ethanol-precipitated part of D. delavayi were sensitive to high temperature and ultraviolet. Conclusion： D. delavayi has definite antibacterial activity in vitro. The water part is the main active part for inhibiting MRSA and E. faecalis， and the 60% ethanol-precipitated part is the primary active part for inhibiting S. aureus. This study provides a theoretical basis for the comprehensive utilization and development of D. delavayi.

Key words: font-family:Times New Roman, ">Dobinea delavayifont-family:Times New Roman, ">；font-family:Times New Roman, "> extractionfont-family:Times New Roman, ">；font-family:Times New Roman, "> antibacterial effectfont-family:Times New Roman, ">；font-family:Times New Roman, "> active partfont-family:Times New Roman, ">；font-family:Times New Roman, "> stability