弱精子症合并超重人群精液PRDX2的表达变化与运动能力的相关性分析

大理大学学报 ›› 2024, Vol. 9 ›› Issue (2): 67-71.

弱精子症合并超重人群精液PRDX2的表达变化与运动能力的相关性分析

胥国麟，叶晓林，钱体军，王云涛，赵文珍*

（大理大学基础医学院，云南大理　671000）

收稿日期:2023-02-08 修回日期:2023-04-13 出版日期:2024-02-15 发布日期:2024-02-24
通讯作者: 赵文珍，教授，E-mail：zwz6565@qq.com。
作者简介:胥国麟，硕士研究生，主要从事男性生殖研究。
基金资助:
国家自然科学基金项目（82160280）；云南省地方本科高校基础研究联合专项资金项目（202101BA070001-041；
202101BA070001-108；202001BA070001-084）

Correlation Analysis of PRDX2 Expression Changes in Semen and Sperm Motility in Overweight Individuals with Asthenospermia

Xu Guolin, Ye Xiaolin, Qian Tijun, Wang Yuntao, Zhao Wenzhen*

（Pre-clinical College, Dali University, Dali, Yunnan 671000,China）

Received:2023-02-08 Revised:2023-04-13 Online:2024-02-15 Published:2024-02-24

摘要/Abstract

摘要： 目的：初步探究PRDX2在弱精子症合并超重人群患者精子的表达情况及其与运动能力的相关性。方法：收集大理大
学第一附属医院生殖科精液常规检查的101份精液标本，将其进行分组，符合正常体质量及正常活力精子的精液标本纳入正
常组（60份），符合体质量超重及弱精子症的精液标本纳入弱精超重组（41份）；经精子自动分析仪检测精液常规参数，间接免
疫荧光法检测PRDX2在2组精子中的定位与表达量变化，并运用相关软件分析PRDX2的变化与精液参数的相关性。结果：
间接免疫荧光结果显示，PRDX2主要定位在人精子中段线粒体处，弱精超重组人精子中PRDX2的荧光表达量显著低于正常
组，且PRDX2荧光表达量与体重指数呈负相关（r=-0.857，P=0.002），与前向运动精子百分数呈正相关（r=0.827，P=0.003）。
结论：弱精子症合并体质量超重人群精子的PRDX2表达下降，且其表达与精子活力正相关。

关键词: 弱精子症, PRDX2, 超重, 精子活力

Abstract: Objective: To explore the expression of PRDX2 in sperm of asthenospermia individuals with overweight and its correlation
with sperm motility. Methods: A total of 101 semen samples from routine semen analysis at the reproductive medicine department of
the First Affiliated Hospital of Dali University were collected. Samples were grouped into a normal group （60 samples） with normal
body mass and sperm vitality and an overweight asthenospermia group （41 samples） with overweight and asthenospermia. Sperm
routine parameters were measured using a sperm automatic analyzer, and the localization and expression of PRDX2 in sperm from the
two groups were detected by indirect immunofluorescence. Correlation analysis was performed using relevant software to assess the
relationship between PRDX2 expression changes and semen parameters. Results: Indirect immunofluorescence showed that PRDX2
was mainly localized in the midpiece mitochondria of human sperm. The fluorescence expression of PRDX2 in sperm from the
overweight asthenospermia group was significantly lower than that in the normal group. PRDX2 fluorescence expression was negatively
correlated with body mass index （r=-0.857, P=0.002） and positively correlated with progressive motility percentage （r=0.827, P=
0.003）. Conclusion: The expression of PRDX2 in sperm is decreased in overweight individuals with asthenospermia, and its
expression is positively correlated with sperm motility.

Key words: asthenospermia, PRDX2, overweight, sperm motility

中图分类号:

R698.2

胥国麟, 叶晓林, 钱体军, 王云涛, 赵文珍. 弱精子症合并超重人群精液PRDX2的表达变化与运动能力的相关性分析[J]. 大理大学学报, 2024, 9(2): 67-71.

Xu Guolin, Ye Xiaolin, Qian Tijun, Wang Yuntao, Zhao Wenzhen. Correlation Analysis of PRDX2 Expression Changes in Semen and Sperm Motility in Overweight Individuals with Asthenospermia[J]. Journal of Dali University, 2024, 9(2): 67-71.

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