LINC00460-miR143-COL1A1 表达调控在结直肠癌细胞增殖、#br#
迁移和侵袭的作用机制研究

doi:10. 3969 / j. issn. 2096-2266. 2025. 08. 006

大理大学学报 ›› 2025, Vol. 10 ›› Issue (8): 41-50.DOI: 10. 3969 / j. issn. 2096-2266. 2025. 08. 006

LINC00460-miR143-COL1A1 表达调控在结直肠癌细胞增殖、#br# 迁移和侵袭的作用机制研究

齐晓鹏，程　伟，田　畅，陶　伦，罗晓红，舒武英*

（大理大学第一附属医院普外一科，云南大理　671000）

收稿日期:2024-01-25 修回日期:2024-04-22 出版日期:2025-08-15 发布日期:2025-09-06
通讯作者: 舒武英，副主任医师，E-mail：215521806@qq.com。
作者简介:齐晓鹏，硕士研究生，主要从事胃肠肿瘤研究。
基金资助:
云南省地方本科高校基础研究联合专项资金项目（201901O070147）；大理大学第一附属医院临床医学学科队
伍建设项目（DFYGG2022-17）

The Mechanism of LINC00460-miR143-COL1A1 Expression Regulation in the Proliferation, Migration,#br# and Invasion of Colorectal Cancer Cells

Qi Xiaopeng, Cheng Wei, Tian Chang, Tao Lun, Luo Xiaohong, Shu Wuying*

（Department of General Surgery, The First Affiliated Hospital of Dali University, Dali, Yunnan 671000, China）

Received:2024-01-25 Revised:2024-04-22 Online:2025-08-15 Published:2025-09-06

摘要/Abstract

摘要： 目的：旨在探讨LINC00460 在结直肠癌中的作用机制及其对细胞增殖、迁移和侵袭能力的影响。方法：通过对TCGA
数据库中结直肠癌患者组织样本进行lncRNA-miRNA-mRNA的竞争性内源RNA调控网络分析，并采用细胞培养、慢病毒转
染、CCK-8试剂盒检测、划痕试验、Transwell侵袭实验以及RT-PCR和qPCR等技术进行体外实验研究。结果：LINC00460 在
结直肠癌组织中呈显著高表达，且其表达与miR143 呈负相关，与COL1A1 mRNA呈正相关。沉默LINC00460 表达可显著降
低结直肠癌细胞的增殖、迁移和侵袭能力，而过表达LINC00460 则可显著提高正常结肠上皮细胞的增殖、迁移和侵袭能力。
结论：LINC00460 可能通过靶向竞争性抑制miR143，促进COL1A1 mRNA表达，从而促进结直肠癌细胞的增殖、迁移和侵袭，有
望成为结直肠癌治疗的潜在靶点。

关键词: 结直肠癌, LINC00460, miR143, COL1A1 mRNA

Abstract: Objective: To investigate the role of LINC00460 in the mechanism of colorectal cancer and its impact on cell proliferation,
migration, and invasion capabilities. Methods: The competitive endogenous RNA regulatory network of lncRNA-miRNA-mRNA in
colorectal cancer patient tissue samples from the TCGA database was analyzed. In vitro experimental studies were conducted using
cell culture, lentiviral transfection, CCK-8 kit detection, scratch assay, Transwell invasion assay, as well as RT-PCR and qPCR
techniques. Results: LINC00460 was significantly upregulated in colorectal cancer tissues, with its expression negatively correlated
with miR143 and positively correlated with COL1A1 mRNA. Silencing LINC00460 expression significantly decreased the
proliferation, migration, and invasion of colorectal cancer cells, whereas LINC00460 overexpression significantly enhanced the
proliferation, migration, and invasion of normal colonic epithelial cells. Conclusion: LINC00460 may promote the proliferation,
migration, and invasion of colorectal cancer cells by competitively inhibiting miR143 （as a target） and upregulating COL1A1 mRNA
expression, and thus may serve as a potential therapeutic target for colorectal cancer.

Key words: colorectal cancer, LINC00460, miR143, COL1A1 mRNA

中图分类号:

R735.3

齐晓鹏, 程　伟, 田　畅, 陶　伦, 罗晓红, 舒武英. LINC00460-miR143-COL1A1 表达调控在结直肠癌细胞增殖、#br# 迁移和侵袭的作用机制研究[J]. 大理大学学报, 2025, 10(8): 41-50.

Qi Xiaopeng, Cheng Wei, Tian Chang, Tao Lun, Luo Xiaohong, Shu Wuying. The Mechanism of LINC00460-miR143-COL1A1 Expression Regulation in the Proliferation, Migration,#br# and Invasion of Colorectal Cancer Cells[J]. Journal of Dali University, 2025, 10(8): 41-50.

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LINC00460-miR143-COL1A1 表达调控在结直肠癌细胞增殖、#br# 迁移和侵袭的作用机制研究

The Mechanism of LINC00460-miR143-COL1A1 Expression Regulation in the Proliferation, Migration,#br# and Invasion of Colorectal Cancer Cells

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