紫外联合红外光谱指纹图谱整合评价三黄片质量

›› 2017, Vol. 2 ›› Issue (6): 31-36.

紫外联合红外光谱指纹图谱整合评价三黄片质量

1.大理大学药学与化学学院，云南大理 671000；2.沈阳药科大学药学院，沈阳 110016

收稿日期:2016-10-18 出版日期:2017-06-15 发布日期:2017-06-15
作者简介:王燕，高级实验师，博士，主要从事中药质量标准和体内药物分析研究.
基金资助:
国家自然科学基金资助项目（81560695）；大理大学博士科研启动基金资助项目（KYBS201512）

The Integrated Use of Ultraviolet Spectrum Fingerprint Coupled with Infrared Spectrum Fingerprint to Comprehensively Assess the Quality Grades of San-huang Tablets

（1. College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China；2. College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China）

Received:2016-10-18 Online:2017-06-15 Published:2017-06-15

摘要/Abstract

摘要： 目的：整合评价不同来源的30批三黄片样品质量。方法：建立三黄片紫外指纹图谱和红外指纹图谱检测方法，将两种分析方法结果取均值整合后评价样品质量。结果：整合后的大部分样品质量等级均在合格范围内（质量等级≤5）。结论：任何一种检测方式都具有单一片面性，不能全面表征样品真实质量，需采用多种分析方法并用、全方位整合的技术手段才能客观综合地对其进行质量评价，为中药在其复杂组分未完全分离的情况下，提供了一种简捷快速、全面客观的定性定量质控研究方法。

关键词: 三黄片, 紫外指纹图谱, 红外指纹图谱

Abstract: Objective: To comprehensively evaluate the quality grades of 30 batches of San-huang tablets from diverse commercial brands. Methods: Ultraviolet spectrum fingerprint and infrared spectrum fingerprint were developed for the measurement of Sanhuang tablets. To evaluate the quality of the drug samples, SL, ML and α values were computed for each analytical method, and the integrated SL, ML and α values were also calculated by taking the average value of the above-established method. Results: Most of the samples were qualified（quality grade≤5）after the use of integrated method. Conclusion: Any detection method is single and onesided, and could not overall characterize the tested samples, which reminds us that it is essential to adopt multi-approaches to disclose the quality information of traditional Chinese medicine and herbal preparations. The strategy proposed in this study provides a simple, fast, comprehensive and objective approach for the quality control of traditional Chinese medicine and herbal preparations from both the qualitative and quantitative aspects, in the case that complex components are not yet completely separated.

Key words: San-huang tablets, ultraviolet spectrum fingerprint, infrared spectrum fingerprint

中图分类号:

O657

王燕，孙国祥. 紫外联合红外光谱指纹图谱整合评价三黄片质量[J]. , 2017, 2(6): 31-36.

Wang Yan，Sun Guoxiang. The Integrated Use of Ultraviolet Spectrum Fingerprint Coupled with Infrared Spectrum Fingerprint to Comprehensively Assess the Quality Grades of San-huang Tablets[J]. , 2017, 2(6): 31-36.

参考文献

〔1〕国家药典委员会.中华人民共和国药典：一部〔M〕.北京：中国医药科技出版社，2015. 〔2〕高苏亚，党高潮，李华. 高效毛细管区带电泳法测定三黄片中黄芩苷〔J〕. 中草药，2007，38（7）：1012-1014. 〔3〕崔书亚，黄铱泠. 循环伏安法测定三黄片中黄芩苷的含量〔J〕. 绵阳师范学院学报，2008，27（11）：48-50. 〔4〕FENG Y L，YU B Y，DONG X P. Simultaneous determina? tion of three kinds of components in sanhuang tablets by high-performance liquid chromatography〔J〕. Acta Pharma? ceutica Sinica，2006，41（3）：285-288. 〔5〕ZHONG S. Simultaneous Determination of Six Active Ingre? dients of Sanhuang Tablet by HPLC〔J〕. Asian Journal of Chemistry，2013，25（15）：8549-8551. 〔6〕解军波，张彦青，戚务勤. RRLC-MS/MS法测定三黄片中小檗碱、黄芩苷和大黄素〔J〕. 中草药，2010，41（5）： 739-741. 〔7〕王钰莹，冯伟红，杨菲，等.“一测多评”法测定三黄片中的大黄蒽醌类成分〔J〕. 中国中药杂志，2012，37（2）： 212-217. 〔8〕LIU Y，BI H，YOU F，et al. Determination of the fingerprint chromatogram of Sanhuang tablet by HPLC〔J〕. Asian Jour? nal of Chemistry，2012，24（10）：4522-4524. 〔9〕DU Q，TANG D Q，YANG D Z，et al. Combination of Fin? gerprint Analysis and Multiple Compounds Determination for the Quality Control of Sanhuang Tablet by HPLCDAD 〔J〕. Latin American Journal of Pharmacy，2012，31 （9）：1330-1340. 〔10〕张艳菊，吴清，倪健，等. 不同厂家三黄片HPLC指纹图谱的比较〔J〕. 世界科学技术-中医药现代化，2012，14 （3）：1656-1662. 〔11〕郑笑为，钟家亮，冯媛媛，等. 中成药三黄片的X射线衍射Fourier指纹图谱研究〔J〕. 中国药事，2007，21（10）： 813-815. 〔12〕孙国祥，豆小文，杨兰萍，等. 高效液相色谱-线性指纹定量法评价人参归脾丸的三波长指纹图谱〔J〕. 色谱， 2013，31（5）：456-461.