大理大学学报 ›› 2019, Vol. 4 ›› Issue (2): 27-.

• 药学 • 上一篇    下一篇

美洲大蠊提取物CⅡ-3 UPLC指纹图谱研究

周姣姣,张成桂,刘玉雨,李若诗,刘光明,夏从龙   

  1. (1.大理大学药学与化学学院,云南大理671000;2.云南省昆虫生物医药研发重点实验室,
    云南大理671000)
  • 收稿日期:2018-10-03 修回日期:2018-10-24 出版日期:2019-02-15 发布日期:2019-02-15
  • 通讯作者: 夏从龙,教授,E-mail:long7484@126.com.
  • 作者简介:周姣姣,硕士研究生,主要从事药物化学研究.
  • 基金资助:
    国家自然科学基金资助项目(81660605)

UPLC Fingerprints of Periplaneta americana Extract CⅡ-3

Zhou Jiaojiao, Zhang Chenggui, Liu Yuyu, Li Ruoshi, Liu Guangming, Xia Conglong   

  1. (1.College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China; 2.Yunnan Provincial Key Laboratory of
    Entomological Biopharmaceutical R&D, Dali, Yunnan 671000, China)
  • Received:2018-10-03 Revised:2018-10-24 Online:2019-02-15 Published:2019-02-15

摘要: [摘要]目的:建立美洲大蠊提取物CⅡ-3超高效液相色谱(Ultra Performance Liquid Chromatography,UPLC)指纹图谱,评价不
同批次CⅡ-3样品的质量。方法:采用Phenomenex Gemini 5u C18 110A(250 mm × 4.6 mm)色谱柱分析美洲大蠊提取物CⅡ-3;
柱温28 ℃;检测波长214 nm;流动相为乙腈-水(0.1%甲酸),进行梯度洗脱。依据相似度评价结果和化学计量学方法进行不同
批次CⅡ-3质量评价。结果:建立了12批CⅡ-3指纹图谱,标定共有峰21个,其中11批样品相似度大于0.900。结论:首次建立
了美洲大蠊提取物CⅡ-3的UPLC指纹图谱,为CⅡ-3质量控制提供科学依据。

关键词: [关键词]美洲大蠊, CⅡ-3, 指纹图谱, 相似度

Abstract:

〔Abstract〕Objective: To establish UPLC fingerprints of Periplaneta americana to assess the quality of different batchs of CⅡ-3
samples. Methods: The analysis of Periplaneta americana extract CⅡ-3 was performed in Phenomenex Gemini 5u C18 110A column
(250 mm × 4.6 mm)and the column temperature was 28 ℃. And acetonitrile-water(0.1% formic acid)was the mobile phase of
gradient elution, set at 214 nm wavelength. The quality of C Ⅱ- 3 was assessed according to the similarity evaluation and the
chemometrics method. Results: The UPLC fingerprints of 12 batches of CⅡ-3 were established. There were 21 common peaks and
fingerprints similarity of 11 batches samples was greater than 0.900. Conclusion: It is the first time that the UPLC fingerprint of CⅡ-3
has been established, which provides a scientific basis for the quality control of Periplaneta americana extract CⅡ-3.

Key words: 〔Key words〕Periplaneta americana L., CⅡ-3, fingerprints, similarity