miR-27a和miR-605基因的多态性与严重生精障碍的相关性研究

doi:10. 3969 / j. issn. 2096-2266. 2021. 02. 012

大理大学学报 ›› 2021, Vol. 6 ›› Issue (2): 59-62.DOI: 10. 3969 / j. issn. 2096-2266. 2021. 02. 012

miR-27a和miR-605基因的多态性与严重生精障碍的相关性研究

孙文文1，柯红利1，阿周存2*

（1. 大理大学基础医学院，云南大理671000；2. 大理大学农学与生物科学学院，云南大理671003）

收稿日期:2020-10-05 修回日期:2020-10-20 出版日期:2021-02-15 发布日期:2021-03-17
通讯作者: 阿周存，教授，博士，E-mail：azhoucun@163.com。
作者简介:孙文文，硕士研究生，主要从事男性不育相关基因研究。

Study on the Association between the Polymorphism of miR-27a and miR-605 Genes and Severe#br# Spermatogenesis Impairment

Sun Wenwen1, Ke Hongli1, A Zhoucun2*

（1. Pre-clinical College, Dali University, Dali, Yunnan 671000, China; 2. College of Agriculture and Biology Science, Dali University,
Dali, Yunnan 671003, China）

Received:2020-10-05 Revised:2020-10-20 Online:2021-02-15 Published:2021-03-17

摘要/Abstract

摘要： 目的：研究miR-27a 基因SNP rs895819和miR-605 基因SNP rs2043556的多态性与严重生精障碍的相关性。方法：应
用聚合酶链反应和限制性片段长度多态性分析技术，在318例严重生精障碍患者（严重少精症和无精症）和234例正常男性中，
对SNP rs895819 和SNP rs2043556 的多态性分布进行调查。结果：严重生精障碍患者中rs2043556 的等位基因C 的频率
（36.2% vs. 29.1%，P =0.013，OR =1.383，95%CI 为1.070~1.788）和带有等位基因C的个体（TC/CC）的频率（57.2% vs. 48.3%，P =
0.037，OR =1.433，95%CI 为1.021~2.012）显著高于正常男性，而基因型TT 的频率（42.8% vs. 51.7%，P =0.037，OR =0.698，
95%CI 为0.497~0.980）则显著低于正常男性。在严重生精障碍患者和正常男性之间，rs895819的等位基因频率和基因型频率
分布差异无统计学意义（P＞0.05）。结论：miR-605 基因SNP rs2043556的多态性与严重生精障碍相关，影响严重生精障碍的
发病风险。

关键词: 严重生精障碍, 多态性, miR-27a 基因, miR-605 基因

Abstract: Objective: To investigate the association between the polymorphism of SNP rs895819 in miR-27a gene as well as SNP
rs2043556 in miR-605 gene and severe spermatogenesis impairment. Methods: The polymorphic distribution of SNP rs895819 and
rs2043556 were investigated by using polymerase chain reaction and restriction fragment length polymorphism assay in 318 patients
with severe spermatogenesis impairment （including azoospermia and severe oligospermia） and 234 normal men. Results: The
frequencies of allele C（36.2% vs. 29.1%, P = 0.013, OR = 1.383, 95%CI is 1.070-1.788）and individuals with allele C（TC/CC）
（57.2% vs. 48.3%, P = 0.037, OR = 1.433, 95%CI is 1.021-2.012）were significantly higher in patients with severe spermatogenesis
impairment than normal men, whereas genotype TT（42.8% vs. 51.7%，P =0.037，OR =0.698，95%CI is 0.497~0.980）was significantly
lower than normal men at SNP rs2043556 locus. The differencesinalleleandgenotypefrequenciesofSNPrs8959189werenotsignificant
betweenpatientsand normal men（P>0.05）.Conclusion: The polymorphism of SNP rs2043556 in miR-605 gene may be associated with
severe spermatogenesis impairment and affect the risk of severe spermatogenesis impairment.

Key words: severe spermatogenesis impairment, polymorphism, miR-27a, miR-605

中图分类号:

R394.1

孙文文, 柯红利, 阿周存. miR-27a和miR-605基因的多态性与严重生精障碍的相关性研究[J]. 大理大学学报, 2021, 6(2): 59-62.

Sun Wenwen, Ke Hongli, A Zhoucun. Study on the Association between the Polymorphism of miR-27a and miR-605 Genes and Severe#br# Spermatogenesis Impairment[J]. Journal of Dali University, 2021, 6(2): 59-62.

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miR-27a和miR-605基因的多态性与严重生精障碍的相关性研究

Study on the Association between the Polymorphism of miR-27a and miR-605 Genes and Severe#br# Spermatogenesis Impairment

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