关键词: font-family:宋体, ">幽门螺杆菌；传代培养；方法比较

Abstract: Objective： To compare the growth rate of Helicobacter pylori（H. pylori） Sydney-standard strain（SS1 strain） by different culture methods， and to find a simple and fast culture method for H. pylori. Methods： The SS1 strain was cultured by four methods. Brain-heart infiltration fluid and bacterial fluid were added to Columbia blood plate， and the bacterial fluid was evenly spread with a disposable inoculating loop. Method 1 was to put the Columbia blood plate coated with bacterial liquid into a sealed anaerobic bag equipped with an anaerobic gas production bag. Method 2 was to place the Columbia blood plate coated with the bacterial solution in a sealed culture jar containing deionized water and an anaerobic gas production bag. Method 3 was based on method 2， in which a candle was lit once in the sealed culture jar. Method 4 was based on method 3， in which a candle was lit in the sealed culture jar for many times. The four methods were all completed in a CO2 incubator， and the H. pylori strains were identified by observing colony morphology and Gram staining， and detected by mass spectrometry. Results：H. pylori strains were successfully cultivated by the four methods. When the number of colonies reached the same level， Method 4 takes significantly less time than the other three methods， and the differences were statistically significant （P<0.05）. Conclusion： A simple and inexpensive method of H. pylori subculture has been found， which has strong application prospects and is worthy of promotion.

Key words:

 , Helicobacter pylori">； subculture">； method comparison