J4 ›› 2011, Vol. 10 ›› Issue (6): 1-3.
• 药学 • 下一篇
目的：测定市场上具有代表性的5种天然染黑染发刺中限用物质对苯二胺的含量，并根据含量<6％的行业标准进行品
质及安全性评价。方法：采用高效液相色谱法(HPLC)，Eclipse XDB—C18 色谱柱(4．6 mmxl50 mm，5 μm)，流动相为乙腈：pH 7.7的磷酸-1％三乙醇胺缓冲溶液5：95；流速0.8盘mL/min；检测波长280 nm。结果：对苯二胺的检测浓度在0．025 0-0．400 0 mg/mL范围内线性关系良好(r=0．999 9)；平均加样回收率为99.27％，RSD=I．49％(n=6)。5种染发剂中对苯二胺的含量在0．23％到1．87％不等。结论：5种染发剂中均含对苯二胺，但均未超过限用标准；本法准确、灵敏、重复性好，可作为天然染发剂中对苯二胺含量测定的有效方法。
Objective：To detect the content of p-phenylenediamine in five representative kinds of natural black hair dyes in the market and evaluate the quality and safety with industry standards．Methods： p-phenylenediamine was determined by HPLC with a Eclipse XDB-Cl8 column (4.6mm×150mm，5 μm)．Mobile phase Was 5％acetonitrile-bufer solution of phosphoric acid at velocity 0．8 mL/min．UV detection wavelength was 280nm．Results：The linear range of p-pheny1enediamine was between 0．025 0 and 0．400 0 mg/mL(r=0．999 9)．The average recovery was 99．27％with RSD 1．49％ (n=6)．The content of p-phenylenediamine varied from 0．23％ to 1．87％ in five kinds of hair dyes．Conclusion：P-phenylenediamine was detected in five hair dyes，but none exceeded the limited standard．This method is accurate，sensitive and reproducible and may be applied to detect the content of p-phenylenediamine in the natural hair dyes．
摘要：
目的：测定市场上具有代表性的5种天然染黑染发刺中限用物质对苯二胺的含量，并根据含量<6％的行业标准进行品
质及安全性评价。方法：采用高效液相色谱法(HPLC)，Eclipse XDB—C18 色谱柱(4．6 mmxl50 mm，5 μm)，流动相为乙腈：pH 7.7的磷酸-1％三乙醇胺缓冲溶液5：95；流速0.8盘mL/min；检测波长280 nm。结果：对苯二胺的检测浓度在0．025 0-0．400 0 mg/mL范围内线性关系良好(r=0．999 9)；平均加样回收率为99.27％，RSD=I．49％(n=6)。5种染发剂中对苯二胺的含量在0．23％到1．87％不等。结论：5种染发剂中均含对苯二胺，但均未超过限用标准；本法准确、灵敏、重复性好，可作为天然染发剂中对苯二胺含量测定的有效方法。
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