J4 ›› 2011, Vol. 10 ›› Issue (6): 4-6.
目的：建立尿液中加替沙星的含量测定方法。方法：采用紫外分光光度法，以333 nm作为检测波长。通过5名健康志愿者实验，考察尿药中加替沙星的排泄量。结果：尿液中加营沙星在1．00～36．00 μg／mL浓度范围内线性关系良好(r=0．999 8)，平均方法回收率为98．1％，RSD为2．87％；平均萃取回收率为95．4％，RSD为3．66％，24 h排泄量在44.3％～69．6％。结论：该方法简单、快速、灵敏、准确，可推广使用。
Objective：To determine the content of Gatifloxacin in urine with ultraviolet spe(：trophotometry method．Methods：Urine samples from 5 healthy volunteers were collected．The contents of Gatifloxacin in unne were determined by ultraviolet speetrophotometry with the detective wavelength of 333 nm．Results：Data have shown a good linear relation between Gatifloxacin eotent and absorbance (r=0．999 8) with the Gatifloxacin concentration in 1．00 to 36．00 μg/mL．The average recovery was 98．1％with RSD 2．87％ ; the average
extraction recovery was 95．4％ with RSD 3．66％ ，excretion rate in 24 hours was 443％～69．6％ . Conclusion：The simple，quick，sensitive and accurate method applied here is worthy of promoting in Gatifloxacin determine in urine．
