J4 ›› 2011, Vol. 10 ›› Issue (6): 11-13.
目的：建立拨云锭中龙胆苦苷的含量测定方法。方法：采用高效液相色谱法测定拨云锭中龙胆苦苷的含量，以Agilent
TC-Cl8(4.6 mmx150 mm，5 μm)为分析柱，流动相为甲醇-水，检测波长为274 nm。结果：龙胆苦苷含量在0.219 2~2.192 0 μg时，线性关系良好(r=0．999 8)，平均回收率为97.6％(n=9)，RSD为2.37％。结论：此方法操作便、回收率高，重现性好，可用于本品中龙胆苦苷的含量测定。
Objective：To establish a method to determine the content of gentiopicroside in Boyunding．Methods：The content of gentiopierosidewasdeterminedbyhighperformanceliquid chromatography．Agilent TC-C1 8 (4.6 mm ×150 mm，5 μm ) was used as analytical column，the mobile phase was composed of methanol amt warer，the detection wavelength was 274 nm．Results：The calibration curve has shown a good linear pattern in gentiopicroside content within 0.219 2~2.192 g(r=0．999 8)，the average recovery ratewas 97．6％ (n=9)
and RSD was 2．37％ ．Conclusion：The simple，stable and reproducible properties of this method enable its application in content measuration of gentiopicroside in Boyunding．
