J4 ›› 2012, Vol. 11 ›› Issue (3): 1-4.

• 药学 •    下一篇

炙升麻与几种升麻属植物抗氧化活性研究

目的:了解常见升麻属植物抗氧化活性,探讨中药材升麻炮制过程中酚酸类成分与抗氧化活性的变化。方法:采用DPPH法与HPLC法对8种亚洲、北美洲升麻属植物共计26个样品进行抗氧化活性与酚酸类成分分析,以了解氧化活性与酚酸类成分之间的关系。结果:绝大部分样品IC50>150 μg·mL-1;炙升麻在炮制过程中酚酸类成分会发生变化,且工艺流程对结果影响很大,抗氧化活性也会受到进一步影响。结论:升麻属植物样品总体上抗氧化活性不高;中药材升麻植物来源具有多样性,不同的样品间在抗氧化活性方面存在较大差异;炙升麻在炮制过程中酚酸类成分会发生改变,从而导致其抗氧化活性与原植物相比发生较大变化,是否影响升麻的药性与药效值得进一步研究。   

  1. 1.大理学院药学与化学学院,云南大理 671000;
    2.纽约市立大学莱曼学院生命科学系,美国纽约 NY10468
  • 收稿日期:2012-01-19 出版日期:2012-03-15 发布日期:2012-03-15
  • 作者简介:姜北,博士,教授,主要从事药用植物与药物资源研究.
  • 基金资助:

    云南省中青年学术技术带头人后备人才基金资助项目(2008PY005);教育部留学回国人员科研启动基金资助项目[2009]1001

Antioxidative Activities of Actaea Plants and Processed Shengma

  1. 1. College of Pharmacy and Chemistry, Dali University, Dali ,Yunnan 671000, China;
    2. Department of Biological Science, Lehman College, City University of New York, NY 10468, USA
  • Received:2012-01-19 Online:2012-03-15 Published:2012-03-15

摘要:

目的:了解常见升麻属植物抗氧化活性,探讨中药材升麻炮制过程中酚酸类成分与抗氧化活性的变化。方法:采用DPPH法与HPLC法对8种亚洲、北美洲升麻属植物共计26个样品进行抗氧化活性与酚酸类成分分析,以了解氧化活性与酚酸类成分之间的关系。结果:绝大部分样品IC50>150 μg·mL-1;炙升麻在炮制过程中酚酸类成分会发生变化,且工艺流程对结果影响很大,抗氧化活性也会受到进一步影响。结论:升麻属植物样品总体上抗氧化活性不高;中药材升麻植物来源具有多样性,不同的样品间在抗氧化活性方面存在较大差异;炙升麻在炮制过程中酚酸类成分会发生改变,从而导致其抗氧化活性与原植物相比发生较大变化,是否影响升麻的药性与药效值得进一步研究。

关键词: 升麻属植物, 炙升麻, 抗氧化活性, DPPH, 酚酸类成分

Abstract:

Objective: To better understand the antioxidative activities of the processed Shengma (Zhi Shengma) as well as the common Actaea plants. Methods: Studies on 26 samples of eight Actaea species from both Asia and North America were performed by using DPPH assay and HPLC analysis. Results: Most of the Actaea samples showed weak/no antioxidative activity with IC50> 150 μg·mL-1. Conclusion: The raw extracts of the roots and rhizomes of the Actaea plants only possessed low antioxidative activities.Actaea species is rich in polyphenols and the latter often show certain antioxidative activities. However, since the chemical profiles for polyphenols of Actaea samples are determined by many factors like plant sources, degree of drying, the way to prepare the processed Shengma, the quality and antioxidative activity of both Shengma and processed Shengma are often unstable. Great care should be taken when Shengma or processed Shengma are subjected for bioactive evaluations.

Key words: Actaea(Cimicifuga)plants, processed Shengma, antioxidation, DPPH, polyphenols

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