HPLC同时测定明目上清片中盐酸小檗碱和黄芩苷的含量

J4 ›› 2012, Vol. 11 ›› Issue (6): 12-15.

HPLC同时测定明目上清片中盐酸小檗碱和黄芩苷的含量

目的：建立同时测定明目上清片中盐酸小檗碱和黄芩苷含量的高效液相色谱方法。方法：Agilent Zorbax Exlipse XDB-C₁₈柱（4.6 mm×250 mm，5 μm）；柱温为25 ^oC；流动相为甲醇-0.2%磷酸水溶液（45:55）；流速为1.0 mL·min^-1；检测波长为265 nm。结果：盐酸小檗碱在1.02~20.40 μg·mL^-1内线性关系良好（r=0.999 9）；黄芩苷在4.54~90.80 μg·mL^-1内线性关系良好（r=0.999 8）；盐酸小檗碱、黄芩苷平均加样回收率为99.2%，101.2%，RSD分别为1.1%和1.6%。结论：本方法简便、准确，重现性好，可用于本制剂的质量控制。

大理学院药学与化学学院，云南大理 671000

收稿日期:2011-11-18 修回日期:2012-03-27 出版日期:2012-06-15 发布日期:2012-06-15
作者简介:周萍，教授，主要从事药物质量控制研究.

Simultaneous Determination of Berberine Hydrochloride and Baicalin in Mingmushangqing Tablets by HPLC

College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China

Received:2011-11-18 Revised:2012-03-27 Online:2012-06-15 Published:2012-06-15

摘要/Abstract

摘要：

关键词: 高效液相色谱法, 明目上清片, 盐酸小檗碱, 黄芩苷

Abstract:

Objective:To establish a high performance liquid chromatography（HPLC）method for the simultaneous determination of berberine hydrochloride and baicalin in Mingmushangqing tablets. Methods: HPLC was carried out with indexes of Agilent Zorbax Exlipse XDB-C18（4.6 mm×250 mm, 5 μm）, column temperature at 25 ^oC, the mobile phase of methanol-0.2 % phosphoric acid（55:45）, flow rate at 1.0 mL·min^-1, and detection wavelength at 265 nm. Results：The method showed good linear relationships within the range of 1.02-20.40 μg·mL^-1 （r =0.999 9）for berberine hydrochloride and 4.54-90.80 μg·mL^-1 （r =0.999 8）for baicalin,respectively. The average recoveries were 99.2%（Relative Standard Deviation RSD=1.1%）and 101.2%（RSD=1.6%）. Conclusion:The method described above is simple, accurate and reproducible which could be used for the quality control of Mingmushangqing tablets.

Key words: HPLC, Mingmushangqing tablets, berberine hydrochloride, baicalin

中图分类号:

R917

周萍, 周浓, 张海珠. HPLC同时测定明目上清片中盐酸小檗碱和黄芩苷的含量[J]. J4, 2012, 11(6): 12-15.

ZHOU Ping, ZHOU Nong, ZHANG Hai-Zhu. Simultaneous Determination of Berberine Hydrochloride and Baicalin in Mingmushangqing Tablets by HPLC[J]. J4, 2012, 11(6): 12-15.

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