J4 ›› 2012, Vol. 11 ›› Issue (6): 23-25.
目的：建立小儿金翘颗粒中连翘苷的含量测定方法。方法：采用高效液相色谱法，以0.1%磷酸-乙腈为流动相进行梯度洗脱，流速1.0 mL/min，检测波长277 nm。结果：连翘苷进样量在0.5~5.0 μg范围内呈良好的线性关系，r=0.999 9；加样回收率为99.3%，RSD为0.25%（n=6）。结论：该方法准确可靠，重复性好，可用于小儿金翘颗粒的质量控制。
Objective: To establish a method to determine the content of Forsythin in Xiaoerjinqiao Granules. Methods: High performance liquid chromatography （HPLC）was applied with the condition of 0.1% phosphoric acid-acetonitrile as mobile phase for gradient elution, the flow rate was 1.0 mL/min, the detection wavelength was 277 nm. Results: The calibration curve was linear in the range of 0.5-5.0 μg for Forsythin, r=0.999 9; the average recovery was 99.3%, and relative standard deviation RSD=0.25%（n=6）.Conclusion: The established method is accurate and reliable, and can be used to determine the Forsythin in Xiaoerjinqiao Granules.
