J4 ›› 2011, Vol. 10 ›› Issue (12): 21-23.
目的：采用紫外分光光度法对鸡肉参中总三萜成分的含量进行测定。方法：以齐墩果酸为对照品，以5%香草醛-冰醋酸溶液、高氯酸为显色系统，在480 nm波长处测定样品吸光度。结果：齐墩果酸在0.04~0.24 mg/mL 范围内吸光度与含量成良好的线性关系，其回归方程为：A=0.001 5 C+0.002 3，r=0.999 9，平均回收率为99.37%，RSD =1.075%（n= 6），所测鸡肉参中总三萜含量以齐墩果酸计为1.41%。结论：该方法操作简便、快速，灵敏度高和重现性好，测定结果准确可靠，可用于鸡肉参的质量控制。
Objective: To establish a method for measurement of total triterpene in Jirousheng (Incarvillea mairei) by Ultraviolet spectrophotometry (UV). Methods: Using oleanolic acid as object, and 5% vanillin-glacial acetic acid solution and perchloric acid as color-developing agent, total triterpenes was measured by spectrophotometry at 480 nm wavelength. Results: The oleanolic acid content showed a good linear relationship with absorbance (r=0.999 9) in the range of 0.04-0.24 mg/mL, and the regression equation was A =0.001 5 C+0.002 3. The average recovery was 99.37% and RSD was 1.075%(n=6), Incarvillea mairei contained 1.41% of total triterpene according to the calculation of oleanolic acid content. Conclusion: The method was simple, rapid, high sensitivity, reproducible and reliable for the quality control of Incarvillea mairei.
摘要：
目的：采用紫外分光光度法对鸡肉参中总三萜成分的含量进行测定。方法：以齐墩果酸为对照品，以5%香草醛-冰醋酸溶液、高氯酸为显色系统，在480 nm波长处测定样品吸光度。结果：齐墩果酸在0.04~0.24 mg/mL 范围内吸光度与含量成良好的线性关系，其回归方程为：A=0.001 5 C+0.002 3，r=0.999 9，平均回收率为99.37%，RSD =1.075%（n= 6），所测鸡肉参中总三萜含量以齐墩果酸计为1.41%。结论：该方法操作简便、快速，灵敏度高和重现性好，测定结果准确可靠，可用于鸡肉参的质量控制。
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