TGF-β1，Caspase-3 和WT1在肾活检肾小球表达及临床病理意义

J4 ›› 2014, Vol. 13 ›› Issue (4): 25-29.

TGF-β1，Caspase-3 和WT1在肾活检肾小球表达及临床病理意义

大理学院附属医院，云南大理 671000

收稿日期:2013-10-11 修回日期:2014-01-06 出版日期:2014-04-15 发布日期:2014-04-15
作者简介:张弢，硕士研究生，主要从事肾脏病及风湿免疫病研究.

Expressions and Clinical-pathological Significance of TGF-β₁，Caspase-3 and WT1 in Glomerulonephritis

Affiliated Hospital of Dali University，Dali，Yunnan 671000，China

Received:2013-10-11 Revised:2014-01-06 Online:2014-04-15 Published:2014-04-15

摘要/Abstract

摘要：

目的：探讨转化生长因子β₁（TGF-β₁）、半胱氨酸天冬氨酸蛋白酶3（Caspase-3）及肾母细胞瘤抑癌基因蛋白（WT1）在肾活检肾小球肾炎组织中的表达及其意义。方法：采用Envision法对40例肾小球肾炎组织及7例对照组肾组织进行TGF-β₁、Caspase-3和WT1免疫组织化学染色标记、分析。结果：TGF-β₁、Caspase-3、WT1在各种肾炎组织中均有表达，各实验组与对照组差异有统计学意义（P<0.05）。TGF-β₁在系膜增生性肾小球肾炎组的表达明显低于膜性肾病组（t=-4.131，P=0.002）；Caspase-3在各组别中其表达差异无统计学意义；WT1在大量高蛋白尿组表达低于非大量蛋白尿组（t=-2.130，P=0.046）；其余组别差异无统计学意义。WT1的低表达与TGF-β₁、Caspase-3的高表达相关。结论：TGF-β₁、Caspase-3表达的上调及WT1表达的下调与肾病理改变密切相关，蛋白尿和足细胞减少可能是肾脏病进展的预测因子及肾小球硬化的原因。

关键词: 肾活检, 转化生长因子&beta, ₁, 半胱氨酸天冬氨酸蛋白酶3, 肾母细胞瘤基因蛋白, 蛋白尿, 免疫组织化学

Abstract:

Objective：To explore the expression and significance of transforming growth factor-β1（TGF-β1），cysteinyl aspartate specific proteinase -3 （Caspase -3）and Wilms' tumor suppressor gene 1 （WT1）in renal biopsy tissues of glomerulonephritis.Methods：40 cases of renal biopsy tissues and 7 cases of control renal tissues were detected and analyzed by Envision immunohistochemistry. Results：TGF -β1，Caspase -3 and WT1 expressed in all cases of renal tissues. There were significant differences between each trail grouping and normal control group （P<0.05）. The positive retes of TGF-β1 in group of mesangial proliferative glomerulonephritis were lower than membranous nephropathy（t=-4.131，P=0.002）. Expression of Caspase-3 showed no differences in divided groups. The expression of WT1 in group of massive proteinuria were lower than that non-massive proteinuria
（t =-2.130，P =0.046）. Other designed groups have no significant difference. Meanwhile，higher WT1 expression showed the correlation with lower expression of TGF-β₁ and Caspase-3. Conclusion：Up-regulated expression of TGF-β1 and Caspase-3 and down-regulated expression of WT1 were closely related to pathologic change in kidney. Proteinuria and podcytes loss is probably the predictor of renal disease progression and a cause for glomerulosclerosis.

Key words: renal biopsy, TGF-β1, Caspase-3, WT1, proteinuria, immunohistochemistry

中图分类号:

R446.8

张弢, 张雨, 李小丽, 何敏华. TGF-β1，Caspase-3 和WT1在肾活检肾小球表达及临床病理意义[J]. J4, 2014, 13(4): 25-29.

ZHANG Tao, ZHANG Yu, LI Xiao-Li, HE Min-Hua. Expressions and Clinical-pathological Significance of TGF-β₁，Caspase-3 and WT1 in Glomerulonephritis[J]. J4, 2014, 13(4): 25-29.

参考文献

〔1〕ROBERT N，FOLEY，ALLAN J，et al. The USRDS：
What You Need to Know about What It Can and Can't Tell
Us about ESRD〔J〕. Clin J Am Soc Nephrol，2013，8（5）：
845-851.
〔2〕LIU Z H. Nephrology in China〔J〕. Nat Rev Nephrol，2013，
9（9）：523-528.
〔3〕LOPEZ-HERNANDEZ F J，LOPEZ-NOVOA J M. Role of
TGF-βin chronic kidney disease：an integration of tubular，
glomerular and vascular effects〔J〕. Cell Tissue Res，2012，
347（1）：141-154.
〔4〕TUNCDEMIR M，ATURK M. The effects of angiotensin-II
receptor blockers on podocyte damage and glomerular
apoptosis in a rat model of experimental streptozotocin -
induced diabetic nephropathy〔J〕. Acta histochemica，
2011，113（8）：826-832.
〔5〕SAKAIRI T，ADE Y，KOPP J B. TGF-beta1 reduces
Wilms' tumor suppressor gene expression in podocytes〔J〕.
Nephrol Dial Transplant，2011，26（9）：2746-2752.
〔6〕黎磊石，刘志红.中国肾脏病学〔M〕.北京：人民军医出版
社，2008：8.
〔7〕JENKINS J，BRODSKY S V，SATOSKAR A A，et al. The
relevance of periglomerular fibrosis in the evaluation of
routine needle core renal biopsies〔J〕. Arch pathol lab
med，2011，135（1）：117-122.
〔8〕IWANO M，AKAI Y，FUJII Y，et al. Intraglomerular
expression of transforming growth factor-beta 1（TGF‐β1）
mRNA in patients with glomerulonephritis：quantitative
analysis by competitive polymerase chain reaction〔J〕. Clin
Exp Immunol 1994，97：309-314.
〔9〕王晨静，卫建平，张晓琴，等.PCNA、Caspase-3和Bcl-2在
不同类型IgA肾病中的表达及意义〔J〕.中国现代医生，
2012，50（6）：1-3.
〔10〕YANG B，JOHNSON T S，THOMAS G L，et al. A shift
in the Bax/Bcl-2 balance may activate caspase-3 and
modulate apoptosis in experimental glomerulonephritis
〔J〕. Kidney Int，2002，62（4）：1301-1313.
〔11〕SCHUMACHER V A，SCHI TZER-SCHREHARDT U，
KARUMANCHI S A，et al. WT1 -dependent sulfatase
expression maintains the normal glomerular filtration
barrier〔J〕. J Am Soc Nephrol，2011，22（7）：1286 -
1296.
〔12〕PEIRED A，ANGELOTTI M L，RONCONI E，et al.
Proteinuria Impairs Podocyte Regeneration by Sequestering
Retinoic Acid〔J/OL〕. J Am Soc Nephrol，2013，24（11）：
1756 -1768〔2013 -9 -18〕. http：//jasn. asnjournals.org/
content/early/2013/08/14/ASN.2012090950.
〔13〕GORRIZ J L，MARINEZ-CASTELAO A. Proteinuria：
detection and role in native renal disease progression〔J〕.
Transplant Rev，2012，26（1）：3-13.
〔14〕DEY B R，SUKHATME V P，ROBERTS A B，et al.
Repression of the transforming growth factor-beta 1 gene
by the Wilms' tumor suppressor WT1 gene product〔J〕.
Mol Endocrinol，1994，8（5）：595-602.
〔15〕RULE A D，AMER H，CORNELL L D，et al. The
association between age and nephrosclerosis on renal
biopsy among healthy adults〔J〕. Ann Intern Med，2010，
152（9）：561-567.

[1]	艾志琼，李丽娟，黎勇，闵梦雅，申元英. 大理地区屠宰生猪的戊型肝炎病毒的流行病学调查[J]. , 2018, 3(10): 63-66.
[2]	宋瑜，刘蜻蜻. 超声引导下经皮肾穿刺活检术的临床应用价值分析[J]. , 2017, 2(4): 69-71.
[3]	张元元, 张燕萍, 王书臣, 林加杨, 樊茂蓉. 益肾活血平喘合剂对哮喘大鼠气道TGF β₁表达的影响[J]. J4, 2013, 12(6): 31-33.