泡核桃壳提取物体外抗肿瘤作用研究

J4 ›› 2015, Vol. 14 ›› Issue (8): 15-17.

泡核桃壳提取物体外抗肿瘤作用研究

（大理学院药学与化学学院，云南大理671000）

收稿日期:2014-09-03 出版日期:2015-08-15 发布日期:2015-08-15
作者简介:刘成娇，2011级药物制剂专业本科生.

In Vitro Anti-tumor Effects of Extracts from Juglans sigillata Shell

（College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China）

Received:2014-09-03 Online:2015-08-15 Published:2015-08-15

摘要/Abstract

摘要：

目的：研究泡核桃壳提取物对3株人癌细胞SKOV-3、HepG-2和MGC-803的体外抑制作用。方法：用碱提醇沉法分离
提取核桃壳中抗肿瘤活性有效部位，得到3个组分HTKD（D）、HTKE（E）和HTKF（F），应用改良MTT法测定对人癌细胞的抑制
作用。结果：在高浓度组分E对3株癌细胞的抑制作用均最明显且呈剂量依赖关系，对3株癌细胞半数抑制浓度（IC50）值也最
小。结论：泡核桃壳提取物对体外培养的肿瘤细胞有抑制作用，药效物质主要存在于组分E中。

关键词: 泡核桃壳, 抗肿瘤作用, MTT法

Abstract:

Objective: To study the anti-tumor effect of Juglans sigillata shell extracts on three human tumor cell lines including
SKOV- 3, HepG- 2 and MGC- 803 in vitro. Methods: Alkali extraction and alcohol precipitation were employed to extract the
antitumor activity of effective components HTKD（D）, HTKE（E）and HTKF（F）from Juglans sigillata shell and observe the inhibitory
effects with MTT assays. Results: At highest concentration component E could obviously inhibit the growth of three human tumor cells
with dose-dependence. In addition, component E inhibited the three tumor cell lines with minimal the median inhibitory concentration
（IC50）. Conclusion: The extract of Juglans sigillata shell showed anti-tumor effects and pharmacodynamic material mainly exist in the
component E.

Key words: Juglans sigillata shell, anti-tumor effect, method of MTT

中图分类号:

R285.5

刘成娇，夏盟恺，李杨，王莹. 泡核桃壳提取物体外抗肿瘤作用研究[J]. J4, 2015, 14(8): 15-17.

Liu Chengjiao, Xia Mengkai, Li Yang, Wang Ying. In Vitro Anti-tumor Effects of Extracts from Juglans sigillata Shell[J]. J4, 2015, 14(8): 15-17.

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