Regulation of MiR-155 on THP-1 Macrophage Inflammatory Response Based on NLRP3 Pathway

Abstract

Abstract:

〔Abstract〕 Objective：To investigate the regulatory effect of miR-155 on lipopolysaccharide （LPS）-induced inflammatory response of THP-1 macrophages based on the nucleotide binding oligomerized domain-like receptor 3 （NLRP3） inflammasome pathway.Method： The LPS-induced inflammatory model of THP-1 macrophages was constructed， the cells were divided into blank control group， LPS group, miR-155 overexpression group and miR-155 negative control group, and real-time quantitative polymerase chain reaction detected the expression changes of interleukin 1β （IL-1β）, tumor necrosis factor α （TNF-α） and NLRP3. Enzyme-linked immunosorbent assay measures the secretion levels of IL-1β and TNF-α; Western blot detection of NLRP3 and cysteine-containing aspartate proteolytic enzyme-1 （Caspase-1）. Results：LPS induced the expression of inflammatory cytokines， NLRP3 and miR-155 in cells； overexpression of miR-155 further promoted the expression of inflammatory cytokines， NLRP3 and Caspase-1 proteins.Conclusion：MiR-155 promotes the LPS induced THP-1 macrophage inflammatory response by activating its potential target NLRP3.

Key words:

"> font-size:10.5pt, ">NLRP3 pathway, miR-155, THP-1 cell, inflammation

CLC Number:

Ning Xiaoxian, Shen Yuanying, Guo Le.

Regulation of MiR-155 on THP-1 Macrophage Inflammatory Response Based on NLRP3 Pathway [J]. Journal of Dali University, 2022, 7(10): 32-38.

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