Determination of the Contents of Total Amino Acids in Parts of Orchidaceae Plants in Yunnan

Abstract

Abstract:

Objective：To optimize the extraction technology of total amino acids from parts of Orchidaceae plants in Yunnan and
determine the content. Methods：The best extraction conditions were optimized by orthogonal test，and the content of total amino
acids was determined in 568 nm through Nihydrin colorimetry method. Results：The best extraction conditions were as follows: 20 mL
75% ethanol，3 times of reflux extraction and 60 min for each time. The concentration and the absorbance of the GLY show a good
linear correlation when changing from 0.5 μg/mL to 10 μg/mL，r = 0.999 8. Conclusion：Nihydrin colorimetry method is simple，
accurate，which can be used for the determination of total amino acids in Orchidaceae plant.

Key words: Orchidaceae plant, total amino acids, content determination

CLC Number:

R284

Yu Xiangdong, Fu Mengyu, Chen Luqiong, Yang Shengchun. Determination of the Contents of Total Amino Acids in Parts of Orchidaceae Plants in Yunnan[J]. J4, 2016, 1(8): 20-23.

References

〔1〕常森有，杨耀海. 文山州药用兰科植物资源调查初
报〔J〕. 林业调查规划，2013，38（3）：61-67.〔2〕罗毅波，贾建生，王春玲. 中国兰科植物保育的现状和展
望〔J〕. 生物多样性，2003，11（1）：70-77.
〔3〕陈业高，王红艳. 金石斛属植物化学成分及药理活性研
究进展〔J〕. 时珍国医国药，2005，16（8）：725-727.
〔4〕刘荣森，杨虹琦，黄郁维，等. 植物中游离氨基酸的提取、
纯化及分析方法〔J〕. 河南科技大学学报（自然科学版），
2007，28（3）：76-79.
〔5〕于艳，毛佳宁，时维静，等. 菊花氨基酸提取方法的比
较〔J〕. 安徽科技学院学报，2014，28（4）：30-33.
〔6〕王培培，鲁芹飞，李婧，等. 铁皮石斛中总氨基酸的含量
测定及提取工艺优选〔J〕. 中国实验方剂学杂志，2013，
19（14）：31-33.
〔7〕杨远帆，倪辉，吴黎明. 茚三酮法测定蜂蜜及果葡糖浆中
的氨基酸含量〔J〕. 中国食品学报，2013，13（2）：171-176.
〔8〕朱瑾，李新霞，陈坚. 茚三酮比色法测定蒜氨酸原料药中
总氨基酸的含量〔J〕. 西北药学杂志，2008，23（3）：136-
138.
〔9〕邵金良，黎其万，董宝生. 茚三酮比色法测定茶叶中游离
氨基酸总量〔J〕. 中国食品添加剂，2008（2）：162-165.
〔10〕张凌. 氨基酸含量测定的分光光度法研究〔J〕. 科技信
息（学术版），2006（3）：238-240.
〔11〕刘玉明，吴玉田，柴逸峰. 蓝桉果实中氨基酸成分的含
量测定〔J〕. 中国药房，2007，18（12）：915-916.
〔12〕王斌，张腾霄，宋相周，等. 不同产地板蓝根中多糖及总
氨基酸含量的分析比较〔J〕. 南方农业学报，2014，45
（1）：23-27.

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