Abstract:

Objective: To compare the HPLC fingerprints of diterpenoid compound in pine cones of Pinus yunnanensis Franch from
different habitats, and to analyze the differences among the pine cones from different sampling sites. Methods: 20 pine cone samples of
Pinus yunnanensis Franch from different places in Yunnan were collected and the fingerprints of diterpenoid compound in the pine
cone of Pinus yunnanensis Franch were established through HPLC method with the marking the common peak. Results: High
performance liquid chromatography analysis condition of the fingerprints of diterpenoid compound in pine cones of Pinus yunnanensis
Franch were established and 11 common peaks were confirmed. There are differences among the chromatographic fingerprints of
diterpenoid compound in pine cones of Pinus yunnanensis Franch from different places. Conclusion: HPLC method is used to develop
chromatographic fingerprint of diterpenoid compound in pine cone of Pinus yunnanensis Franch with good resolution of diterpenoid,
which lays the quality specification study foundation of pine cone of Pinus yunnanensis with a stable, accurate and reproducible method.

Key words: Pinus yunnanensis Franch, pine cone, diterpenoid, HPLC method