Journal of Dali University

Study on β-hematin Formation Inhibition Activities of 24 Common-used Herbal Medicines of Bai
Nationality

Shan Hua, Liu Ziqi, Chen Yingzhi, Zhang Panpan, Chen Hao, Jiang Bei

2016, 1(10): 1-4.

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Objective: To study β-hematin formation inhibition activity of 24 herbal medicines often used by Bai nationality and to
provide the experimental evidence for finding new effective antimalarial medicines from natural products. Methods: Plant materials
were extracted with 75% ethanol and distilled water using the reflux method. After the extract solutions were combined and
concentrated under reduced pressure, the resulting residue was completely dried by lyophilization. The dried samples were screened for
antimalarial activity by using β-hematin formation inhibition assay. Bioactivity results were evaluated by IC50 values. Results: Among
the 24 plant samples, water extracts of Onosma paniculatum showed the best activity to inhibit β-hematin formation with the IC50 value
of（1 254.1±3.9）μg/mL. Water extracts of Hemiphragma heterophyllum, Arisaema erubescens and Phyllanthus emblica had weak
activity to inhibit β-hematin formation with the IC50 values over 1 388.9 μg/mL. Conclusion: O. paniculatum, H. heterophyllum, A.
erubescens and P. emblica reveal potential antimalarial activity and deserve further study in the future.

Effect of Extracts form Mirabilis jalapa on the Damaged L-02 Human Embryo Hepatocytes Induced by
Ethanol

Luo Ailian, Cheng Shengbang, Shen Lei, Chen Junya, Guo Meixian

2016, 1(10): 5-8.

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〕Objective: To study the effect of extracts form Mirabilis jalapa on the damaged L-02 human embryo hepatocytes induced
by ethanol. Methods: L- 02 human embryo hepatocytes in logarithmic phase were chosen and damaged by ethanol at a final
concentration of 100 mmol/L. After 8 hours, different concentrations of the extracts form Mirabilis jalapa were added in. After 16 hours
of cultivation, MTT method was used to observe inhibition rate of L-02 human embryo hepatocytes damaged by ethanol, and Elisa
method was used to detect the activity of glutamic- pyruvic transaminase（ALT）, glutamic- oxalacetic transaminase（AST） and
superoxide dismutase（SOD）, and the content of malonaldehyde（MDA）in nutrient solution. Results: 8 mg/mL or higher crude herbal
concentrations of Mirabilis jalapa can significantly decrease inhibition rate of ethanol-induced damaged L-02 cells, obviously reduce
ALT and AST activity, increase SOD activity and decrease MDA content in the culture for damaged cells. Conclusion: Extracts form
Mirabilis jalapa has protective effect on the damaged L-02 cells induced by ethanol.

Difference Analysis of Free Anthraquinones, Combined Anthraquinones and Total Anthraquinones in
Cultivated Rheum palmatum L. in Different Producing Areas

Yang Min, Pan Xingjiao, Jiang Jing, Yao Yuting, Zhao Yang, Zhou Nong

2016, 1(10): 9-13.

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Objective: To determine content of free anthraquinones, combined anthraquinones and total anthraquinones in cultivated
Rheum palmatum L. in different producing areas, and to provide scientific basis for quality assessment and reasonable using. Methods:
Agela Venusil XBP-C18 column（4.6 mm×250 mm, 5 μm）was used with the mobile phase of methanol-0.1% posphoric acid solution
（75：25）at the flow rate of 1.0 mL/min, the detection wavelength was set at 254 nm and the column temperature was 35 ℃. To
determine the content of cultivated Rheum palmatum L. from 11 different producing areas, cluster analysis was carried out for the
results. Results: 5 kinds of anthraquinons exist in cultivated Rheum palmatum L. from different producing areas, in which cultivated
Rheum palmatum L. from Lixian, Gangsu was the best. Cluster analysis divided cultivated Rheum palmatum L. from 11 different
producing areas into 3 categories. Conclusion: This paper analyzes the quality of cultivated Rheum palmatum L. from different
producing areas, it could provide reference for quality assessment and standardized planting of cultivated Rheum palmatum L. in Dali.

Determination of Three Components in XML Injection by HPLC

Shao Weizai, Duan Zhishang,Wan Desheng, Que Yuling, Zhou Yongbin

2016, 1(10): 14-17.

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Objective: To develop a method for determination of uracil, hypoxanthine and inosine of XML injection. Methods: HPLC
method was adopted in the determination of three components in XML injection. The analysis was performed on an Agilent C18 column
with the mobile phase consisting of 0.05 mol/L sodium acetate buffer solution（A）（pH=5.0）, methanol and 0.05 mol/L sodium citrate
butter mixed solution（80：20）（B）（pH=6.5）by gradient elution at a flow rate of 0.5 mL/min. The detection wavelength was set at 254
nm and the column temperature was 30 ℃. Results: The linear ranges were 0.038 to 2.404 μg（r=0.999 97）for uracil, 0.055 to 3.524
μg（r=0.999 94）for hypoxanthine and 0.089 to 5.716 μg（r=0.999 92）for inosine. The average recoveries（n=9）of three components
were 99.30% for uracil, RSD=1.22%; 99.39% for hypoxanthine, RSD=0.91%; and 99.61% for inosine, RSD=0.93%. Conclusion: This
method is simple, convenient and accurate, which is suitable for the quantitative analysis of uracil, hypoxanthine and inosine in the
XML injection.

Study on Fingerprints of Diterpenoid Compound in the Pine Cone of Pinus yunnanensis Franch

Wu Daoxun, Liu Lu, Liu Xi, Ma Jiao, Wang Ying, Zhang Haizhu

2016, 1(10): 18-22.

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Objective: To compare the HPLC fingerprints of diterpenoid compound in pine cones of Pinus yunnanensis Franch from
different habitats, and to analyze the differences among the pine cones from different sampling sites. Methods: 20 pine cone samples of
Pinus yunnanensis Franch from different places in Yunnan were collected and the fingerprints of diterpenoid compound in the pine
cone of Pinus yunnanensis Franch were established through HPLC method with the marking the common peak. Results: High
performance liquid chromatography analysis condition of the fingerprints of diterpenoid compound in pine cones of Pinus yunnanensis
Franch were established and 11 common peaks were confirmed. There are differences among the chromatographic fingerprints of
diterpenoid compound in pine cones of Pinus yunnanensis Franch from different places. Conclusion: HPLC method is used to develop
chromatographic fingerprint of diterpenoid compound in pine cone of Pinus yunnanensis Franch with good resolution of diterpenoid,
which lays the quality specification study foundation of pine cone of Pinus yunnanensis with a stable, accurate and reproducible method.

Analysis of Essential Drug Using Condition in a Middle First-class Hospital form 2013 to 2015

Zhang Yan, Pan Min, Li Yang, Cao Huimin, Wu Xiumei, Yang Xiaoli

2016, 1(10): 23-26.

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Objective: To analyze the essential drug using condition in a middle first-class hospital, and to provide theoretical basis
for the implement and promotion of the essential drug system in medical institutions. Methods: The specifications and the amount of
drug use from 2013 to 2015, as well as anti-microbial, cardiovascular system, respiratory system classes, four drug infusion DDD data
changes were compared. Results: Antibiotic use was effectively controlled, but daily costs remained high. Cardiovascular drug and
infusion drug use increased with variety. Conclusion: Medical institutions should gradually improve essential drug system, disease
trends in the new, rational drug use and strengthen supervision.

Rationality Evaluation of the Prophylactic Use of Proton Pump Inhibitors for Stress Ulcer in Our Hospital

Nie Caixia, Zhao Qizou, Lin Yiju, Zou Zhimu, Zhang Lansheng

2016, 1(10): 27-29.

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Objective: To investigate the prophylactic use of proton pump inhibitors（PPIs）for stress ulcer（SU）in our hospital and to
evaluate its rationality. Methods: 158 medical records of patients from Departments of Neurosurgery, Hepatobiliary Surgery, General
Surgery, Orthopedics and Urology Surgery with using PPIs in January 2015 were sampled for evaluation of indications in prophylactic
medication, usage and dosage, solvents and course of treatment of PPIs. Results: The reasonable utilization rate of PPIs for prevention SU
was 82.91%, while 27 cases were unreasonable and the unreasonable rate was 17.09 %. The medication indications, choice of solvent,
dosage and course were unreasonable, and no evidence application accounted for 41.18 % of unreasonable drug use. Conclusion: The
application of PPIs in the prevention of SU is basically reasonable, but irrational use of PPIs exists, which needs to be improved.

The Correlation between Point Mutation of T3394C in Mitochondrial ND1 Gene and Patients with Type 2
Diabetes Mellitus in Aged Bai Minorities in Dali

YangYongqin,YangZefang,ChenYali,OuyangWenjun,YangJiani,ZhangXiaojuan,XiongWei

2016, 1(10): 30-34.

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Objective: To investigate the correlation between point mutation of T3394C in mitochondrial NADH ND1 gene and
patients with type 2 diabetes mellitus in aged Bai minorities in Dali. Methods: 200 unrelated individuals with type 2 diabetes mellitus
（T2DM group）which were randomly chosen among aged Bai minorities in Dali, Yunnan, and 218 aged cases with normal glucose
tolerance without DM family history（Control group） were inspected. Polymerase chain reaction and restriction fragment length
polymorphism（PCR-RFLP）technology were used for screening. Results: The point mutation T3394C in mitochondrial ND1 gene
were found in 5 cases in T2DM group（2.50%）, while only 1 case in control group（0.46%）, and there were significant differences
between two groups（P<0.05）. Mitochondrial ND1 gene T3394C point mutation in patients with type 2 diabetes mellitus showed
obvious maternal inheritance characteristics. Conclusions: The point mutation of T3394C in the mitochondrial DNA ND1 gene may be
related to the occurrence of T2DM in aged Bai minorities in Dali.

Study on the Sodium Nitrite Injury and Repair Mechanisms to the Male Mice

Zhang Wang, Shen Yuanying, Chen Hongyun, Song Zhengrui, Ji Shoulian, Wang Jinyon

2016, 1(10): 35-38.

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Objective: To study the sodium nitrite injury and repair mechanisms to the male mice. Methods: Kunming species male
mice were used in the experiment. Dose groups were administered by oral with NaNO2（15, 30, 60 mg/（kg·d））, while the control
groups had been given normal saline of the same volume for 14 days. After 24 hours of the last exposure to NaNO2, one-third of mice in
three dose groups were killed. The remaining mice were divided into two groups. One group was given Lyceum barbarum
polysaccharide, while another group was given normal saline of the same volume for 14 days and then killed all the mice. Experiment
measured index included the main organ weight, organ coefficient, serum testosterone level and sperm deformity rate. Results: With
the increasing dose of NaNO2, the mice serum testosterone level and the spleen weight were decreased significantly（all P value＜
0.05）, the sperm deformity rate was increased significantly（all P value＜0.05）. After stopping giving NaNO2, the level of serum
testosterone were increased, the sperm deformity rate and spleen organ coefficient were declined in 15, 30 mg/（kg·d）NaNO2 groups,
the three indexes were statistically significant comparing with previous experiments（all P value＜0.05）. The other indexes had no
change. Conclusion: NaNO2 had the ability to inhibit the level of serum testosterone and induce the sperm deformity in mice. After
stopping exposing to NaNO2, the mice can repair some damages by themselves, the Lyceum barbarum polysaccharide may also have
recovery effect, which needs further study.

The Value of Using Bronchoscopy Instead of Thoracoscopy in the Diagnosis of
Pleural Effusion in Pleural Biopsy

Yang Zhongchuan, Wang Tao, Xiong Zhiju, Zhao Xiongxiu

2016, 1(10): 39-42.

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Objective: To explore the value of diagnosing pleural effusion with bronchoscopy instead of thoracoscopy. Methods:
Clinical data of 42 cases using bronchoscopy instead of thoracoscopy in patients with pleural effusion which remained unsure in pleural
biopsy from August 2013 to October 2014 were analyzed retrospectively. Test results, the clinical data of patients and the correlation
analysis of test results were analyzed. Results: 24 cases were diagnosed as malignant tumor, including 20 cases of lung cancer, 4 cases
of malignant pleural mesothelioma, 12 cases of tuberculous pleurisy, 6 cases of chronic nonspecific inflammation. 6 cases of patients
with unsure diagnosis had half a year follow-up, and there was no deterioration occurred after hospital discharge and no any evidence
of malignant tumor and tuberculosis. Conclusion: Using bronchoscopy instead of thoracoscopy examination in the diagnosis of patients
with pleural effusion of unknown origin is a safe and easy diagnostic method with low false negative rate. Patients with imaging
evidence of a chronic course, shortness of breath, smoking, massive pleural effusion, malignant tumor should be examined by
thoracoscopy.

Blind Spot of Radical Gastrectomy Assisted by Laparoscopy and Countermeasures

Yang Jiwu, Cheng Wei, Zhao Pengju

2016, 1(10): 43-45.

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Objective: To summarize the clinical effect of radical gestretomy assisted by laparoscopy. Methods: 60 patients with
gastric cancer in the first affiliated Hospitals of Dali University were studied, including 30 cases took radical gestretomy assisted by
laparoscopy as the observation group; other 30 cases took the open radical gestretomy as control group. Clinical effects and
complications of two groups were studied. Results: Observation group is better than that of control group in the incision length,
intraoperative blood loss, postoperative exhaust time, postoperative intestinal function recovery time and postoperative into liquid diets
time, length of hospital stay, and the postoperative complications, and the difference was statistically significant （P < 0.05）.
Conclusion: Radical gestretomy assisted by laparoscopy enjoys many advantages comparing with open radical gestretomy: small
trauma, fast recovery, less postoperative complications, which is worth of popularization in clinical surgery.

Study on Hip Replacement in the Treatment of Advanced Femoral Head Necrosis

Wang Jiaming, Li Jiang, Luo Xiangdong, Lu Junqi, Tang Xizhang

2016, 1(10): 46-49.

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Objective: To investigate the clinical effects of hip replacement in the treatment of advanced femoral head necrosis.
Methods: 64 cases of advanced femoral head necrosis received hip replacement surgery were analyzed, as well as recovery eccentricity
and center of rotation during the surgery, and the lower extremity length after recovery were studied. The position of prosthesis and the
situation of bone ingrowth were investigated through X-ray in follow-ups, and the function recovery of hip joint was evaluated by
Harris Scale. Results: The patients were followed up for an average of 4.9 years（6 to 87 months）without death case, including 2 cases
suffered of venous thrombosis of lower extremity; 2 cases suffered hip pain two years after surgery（proved to be loosing artificial limb）,
which were relived by taking painkiller; 62 cases recovered with normal function, no dislocation, no broken prosthesis, and no unequal
lower limb length. Through Harris Scale, the average score was 40 before surgery, 94.5 after surgery. Conclusion: Total hip
replacement can provide effective treatment for advanced femur head necrosis, recover hip function, relieve the pains, and improve the
quality of life.

Investigation on Pollen Allergen in Patients with Allergic Rhinitis in Baoshan, Yunnan

Yang Guoning, Li Minghua, Ma Ya

2016, 1(10): 50-53.

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Objective: To investigate the distribution of pollen allergens in patients with allergic rhinitis（AR）in Baoshan, Yunnan,
and to offer basis for the diagnosis, prevention and treatment. Methods: 980 allergic rhinitis were examined by skin prick test（SPT）
with 15 kinds of pollen antigen extract of allergic rhinitis. Results: Among 980 cases, 269 cases（27.4%）were detected with one
antigen, 460 cases（46.9%）were detected with two or more antigens. The main common pollen allergens in patients with allergic
rhinitis in Baoshan were 348 cases of Gramineae（35.5%）, 310 cases of aestivo-autumnal（31.6%）, 251 cases of Chinese ash
（25.6%）, 2 cases of sakura（0.2%）which is the least one. Positive rate of pollen allergens were different between youngsters group
and middle-aged group. Conclusion: Gramineae, stivo-autumnal and Chinese ash were the main pollen allergens of allergic rhinitis in
Baoshan. Most patients with allergic rhinitis were allergic to two or more kinds of pollen allergens. Skin prick test（SPT）is helpful for
the discovery of the allergens so as to provide important basis of desensitization therapy.

Analysis of the Eye Diseases Screening Results of 933 Newborns by Ret Cam III

Chen Gang, Du Mei, Li Yiqing, Li Meijun, Liu Liqing

2016, 1(10): 54-56.

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Objective: To explore the application value of Ret Cam III in eye diseases screening in newborns, and to understand the
oncome situation of fundus diseases for providing basis for the prevention and treatment of eye diseases in newborns. Methods: Ret
Cam III was used to screen eye diseases in 933 newborns in this hospital from March 2015 to August 2015 and the discussion and
analysis were carried out for the results. Results: 87 cases of fundus diseases were detected among 933 newborns（9.32%）, including
64 cases of fundus hemorrhage（6.86%）; 18 cases of retinopathy in premature infants（1.93%）; 5 cases of other eye diseases（0.54%）;
2 cases of optic atrophy（0.21%）; 2 cases of familial exudative vitreoretinopathy（0.21%）; 1 case of permanent hyaloid artery stump
（0.11%）. Conclusion: Because fundus diseases in newborns are not easy to be noticed at early stage, so it's necessary to pay attention
to the screening, especially for the premature infants, and full term infants with high risk factors. Ret Cam III is easy to operate with
wide range of observation, which is beneficial for promoting the diagnosis rate of fundus diseases in newborns, and for noticing and
treating the diseases at early stage effectively.

Influence of Laser-assisted Hatching on the Potential of Elderly Women's
Frozen-thawed Embryo Implantation

Li Zhengwei, Xu Yijuan, Zhang Ruopeng

2016, 1(10): 57-60.

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Objective: To analyze the influence of laser-assisted hatching technology on the potential of elderly women's freezethawed
embryo transfer. Methods: 427 patients were grouped by age and assisted hatching（AH）. Comparison of embryos implantation
rate and clinical pregnancy rate were carried out among groups. Results: Among <35 years old patients, two groups' embryos
implantation rate of AH were higher than non-AH groups with statistically significant, but there was no difference in clinical pregnancy
rate and statistic; among ≥35 years old patients, embryo implantation rate and clinical pregnancy rate of AH group were higher than
non-AH group, and were statically significant. Conclusion: The application of laser-assisted hatching technology can increase clinical
pregnancy rate in elderly patients, especially in greatly promoting implantation rate of frozen-thawed embryo.
laser-assisted hatching|elderly women|frozen-thawed embryo|transplant; implantation rate|clinical pregnancy rate

The Effect of Different Endometrium Preparations Programs on the Transfer Outcome of
the Frozen-thawed Embryo

He Pei, Li Zhengwei, Xu Yijuan, Zhang Ruopeng

2016, 1(10): 61-64.

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Objective: To analyze the clinical effects of different endometrium preparation programs on the transfer of frozen-thawed
embryo for providing basis for the selection of suitable subintimal preparation programs and improving clinical pregnancy rate.
Methods: Based on the clinical data of 118 infertile couples cases with 197 cycles from January 2014 to March 2015, the endometrium
preparation programs were divided into three groups（Group A: natural cycle group; Group B: stimulate ovulation solutions group;
Group C: hormone replacement group）. The difference of endometrial thickness, gonadal hormone concentrations（luteinizing hormone-
LH, progesterone-P, estradiol-E2）, clinical pregnancy rate, single fetus and twin pregnancy rate, and the rate of abortion among three
groups were compared. Results: Among group A, B and group C, endometrial thickness, LH, P are statistically significant（P<0.05）.
Estradiol（E2）levels of three groups have no significant difference（P>0.05）. Clinical pregnancy rate, single fetus and twin pregnancy
rates, rate of abortion of three groups have no statistical significance. Conclusion: Endometrial thickness, LH, different levels of of
three groups under the programs of endometrial prepartions have no significant effect on clinical pregnancy rate. Three endometrial
preparation programs have no significant effect on the transfer outcome of the frozen-thawed embryo.

Application Value of Color Doppler Ultrasound in Congenital Muscular Torticollis

Zhang Xiaodong

2016, 1(10): 65-66.

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Objective: To explore the application value of Color Doppler Ultrasound examination in infant congenital muscular
torticollis. Methods: The performance of Color Doppler high frequency ultrasound on the neck of 9 cases with clinically or
pathologically confirmed congenital muscular torticollis were retrospectively analyzed. Results: Ultrasonography show masses echo in
children with congenital muscular torticollis for sternocleidomastoid muscle, with distribution along the long axis of the muscle. CDFI:
muscle stripes within the region can display dot color flow signal, and there was a significant difference comparing with contralateral
normal chest lock papillary muscle. Conclusion: Color Doppler Ultrasound has high diagnostic value of congenital muscular torticollis.

PPAR-α Expression of Hepatocytes after TAE in Liver Cancer

Zhang Liang,Wang Guangming, Tang Yanlong, Liu Ling, Du Wei

2016, 1(10): 67-70.

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Objective: To investigate the function mechanism of PPAR -α in liver cancer on abnormal liver function after
transcatheter arterial embolization（TAE）. Methods: VX2 hepatic cancer model in 26 New Zealand white rabbits model were
established with 86% successful rate（24/28）. VX2 hepatic cancer models in rabbits were randomly divided to embolization group
（12）, angiography group（12）. Rabbits of very group were sacrificed 8 hours after TAE. Liver tissues surrounded HCC were collected
to make paraffin section, and the expression of PPAR-α in hepatic tissue was detected after hematoxylin & eosin（HE）staining and
immunohistochemistry. Results: Positive cells in embolization group ranged from 51%~85% with a positive assessment ++ / +++;
positive cells in angiography group were between 10%~30% with a positive assessment of + / ++. Statistical analysis showed that the
PPAR-α expression in cancer adjacent hepatic tissue of embolization group was significantly higher than angiography group（P＜
0.05）. Conclusion: After TAE, PPAR-α expression in tumor adjacent hepatic tissue increased significantly. The possible reason of
high expression of PPAR-α might be hypoxia ischemia of cancer adjacent normal tissues.

The Value of ADC Value In Hypoxic-ischemic Encephalopathy Diagnosis

Xu Kun, Zhang Chengzhi

2016, 1(10): 71-72.

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Objective: To explore the value of dorsal thalamus ADC value in diagnosis of Hypoxic-ischemic Encephalopathy（HIE）
in newborns of different age in days. Methods: Dorsal thalamus ADC value of 41 cases of neonatal HIE in the first Affiliated Hospital
of Dali University in May 2015 to October 2015 were measured by ANOVA statistical analysis. Results: Dorsal thalamus ADC value of
mild, moderate and serious groups were significantly different. The normal ADC value is lower than mild, the mild ADC value is higher
than moderate group, and the moderate ADC value is higher than serious group. Conclusion: The dorsal thalamus ADC value has
significance in MRI diagnosis of normal, mild, moderate and serious neonatal HIE.

Investigation on the Carrier Rate of Hepatitis B Virus of College Students in a University in Yunnan

Wang Tao, Zhang Lei, Pan Yunhua, Wu Lixian, Bai Li, Wu Youcong

2016, 1(10): 73-76.

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Objective: To investigate the Hepatitis B Virus（HBV）carrier rate of college students in a university in Yunnan.
Methods: Enzyme linked immunosorbent assay（ELISA）was used for detection of HBsAg in the serum with SPSS18.0 statistical
analysis. Results: Among 1 964 college students, 67 were diagnosed as HBsAg positive（3.4%）, including 36 were male students
（5.0%）, 31 were female students（2.5%）（χ2=8.649, P=0.004）; 51 were Han nationality（3.5%）, 16 were ethnic minorities（3.2%）
（χ2=0.129, P=0.778）; 36 came from Yunnan Province（2.6%）, 31 were from provinces other than Yunnan（5.5%）（χ2=10.156, P=
0.002）; 20 were from cities（2.7%）, 47 from rural area（3.9%）（χ2=1.948, P=0.200）. Conclusion: The HBV carrier rate of college
students in this university was significantly lower than the national average level, the HBV carrier rate of male students was higher than
that of female students, the HBV carrier rate of students from other provinces was higher than that of students in Yunnan.

Investigation on the Infection of HIV, HCV and Association High Risk Factors among Drug Users

Li Ze, Liu Jizheng, Shen Yuanying

2016, 1(10): 77-80.

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Objective: To understand the prevalence of HIV, HCV and estimate high risk factors among drug users in Dali Prefecture,
thereby to provide the scientific basis for promoting and perfecting the prevention work more accurately in a larger range. Methods:
The blood samples of 423 new drug users in Dali Detoxification Center from January 2015 to June 2015 were collected to detect the
infection status of HIV and HCV. Using the data in questionnaire survey and statistical methods, risk factors related to infection were
analyzed. Results: The infection rates among total 423 drug users were 19.4%（82/423）for HIV, 46.3%（196/423）for HCV. It is
found that 96.3%（79/82）of the 82 HIV positive had infected with HCV. Multiple factor analysis indicated that the prevalence of HIV
and HCV among drug users were positively related to drug using time, injection drug use, sharing needles. In addition, HCV infection
was associated positively with the age of drug users and the times and quantity of drug addicts using per day. Conclusion: All sorts of
high risk behavior associated with HIV and HCV infection result in the high HIV and HCV prevalence among drug users in Dali, which
accelerate the HIV and HCV prevalence among this group of people.

Establishment of Hepatitis A Virus RT-PCR Detection Method and
Its Epidemiological Investigation in Swinery

Ai Zhiqiong, Zhang Ling, Li Lijuan, Li Ze, Wang Yunhong, Shen Yuanying

2016, 1(10): 81-84.

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Objective: To study the epidemic state of the Hepatitis A Virus（HAV）infection in swinery, and to explore the possibility
of swine as another host or carrier of HAV for providing basis for prevention and controlling measures of human HA. Methods: Serum
HAV-IgG of swine in Dali was detected by enzyme-linked immunosorbent assay（ELISA）, and HAV-RNA of swine blood and swine
bile samples were tested by reverse transcription- polymerase chain reaction（RT-PCR）, with the blood and bile of HAV as positive
control. Results: ①HAV antibody positive rate of the blood samples of swine were detected as 73.3%（374/510）; ②RT-PCR method
was built to detect HAV; ③HAV-RNA of positive control samples were detected with RT-PCR; ④HAV-RNA of swine blood and
swine bile samples were not detected with RT-PCR. Conclusion: RT-PCR method is successfully built to detect HAV-RNA. The
antibody positive rate of HAV is rather high in the blood sample of swine. Further research is necessary to demonstrate whether the
swine could infect human HAV.

Observation and Nursing of Coronary Artery Dilatation Patients Receiving Anti-thrombosis Therapy

Chen Yu, Zhang Fengming, Li Jingzhu, Long Jing, Yang Jianmei

2016, 1(10): 85-87.

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Objective: To investigate the nursing tips of coronary artery dilatation patients using aspirin and clopidogrel, and for
making sure the medication safety. Methods: 188 coronary artery dilatation patients' bleeding situation were observed and nursed, as
well as had received mental nursing, diet nursing and discharge instruction. Results: Among 188 patients, 150 patients had no
bleeding, 2 patients had gastrointestinal hemorrhage, 7 patients had bleeding gums, 3 patients had epistaxis, 2 patients had gross
hematuria, 4 patients had subcutaneous hemorrhage. Conclusion: Effective nursing can reduce and prevent the adverse reaction and
complications.

Application of PDCA Cycle in Reasonable and Effective Shortening the Average Hospitalization Days

Yang Taoxian, Li Rui, Ma Li, Li Rui, Li Wei

2016, 1(10): 88-90.

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Objective: To explore the application effect of PDCA cycle in reasonable and effective shortening the average
hospitalization days. Methods: A quality improvement project team was set up to introduce P（plan）D（do）C（check）A（action）
management method for the management of average hospitalization days. Results: Through data statistical analysis, it's found that the
average hospitalization days gradually reduce after using PDCA cycle theory in the management. Conclusion: PDCA cycle theory is of
great significance in the reasonable and effective shortening average hospitalization days, which is worth of promoting.

Exploration of Strengthening Students Research Training in Pharmacology Teaching

Sun Xiaofei, Xiong Lei, Lei Na, Yao Xuezhou, Xie Yuhuan

2016, 1(10): 91-93.

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In order to promote the pharmaceutical specialty students' research quality and ability, a research training mode was
designed for pharmaceutical college students based on the pharmacology experiment teaching platform. According to the demands of
professional development and training program, the training modes based on theory and practice as well as science research were built
respectively. The former would consolidate the basic theory knowledge and practice the basic research skills. The latter would cultivate
the upperclassmen's capacity for scientific research based on research subjects. Through the training, the trained students were overall
satisfied and their research ability were general improved. Exploration was made by this training mode based on the pharmacology for
improving scientific research ability of pharmaceutical students, for laying a foundation for students' future research work.

Analysis of Articles and Authors in Journal of Dali University（Medical Sciences）from 2011 to 2015

Li Yang

2016, 1(10): 94-97.

Asbtract ( 134 )

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By using the method of literature metrology, a statistical analysis of articles and authors in Journal of Dali University
（Medical Sciences）from 2011 to 2015 was made, as well as comparison with the literature. The results show that the number is
relatively stable; the paper number has increased year by year since the nursing column opened; the average publication delay shows a
gradual increase; fund papers account for 34.11%, national fund papers are 7.36%; first author with senior title account for 31.11%;
authors from other universities and institutions are mainly in Yunnan, authors from other provinces are only about 3.18%.

Situation, Problems and Countermeasures of the Education of MBBS International Students from
South Asia in Dali University

Wang Bin, Liu Ming, Zhang Rumei, Yang Ying, Hu Jifu

2016, 1(10): 98-100.

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In recent years, the education of MBBS international students from South Asia in Dali University has achieved a great
success, but there are remaining problems and difficulties in training mode, curriculum setting and faculty. Based on the actual
situation of Dali University, it's suggested to push forward the education for South Asia students with constructing scientific and
reasonable professional training mode as the starting point.

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