Establishment of Hepatitis A Virus RT-PCR Detection Method and
Its Epidemiological Investigation in Swinery

Abstract

Abstract:

Objective: To study the epidemic state of the Hepatitis A Virus（HAV）infection in swinery, and to explore the possibility
of swine as another host or carrier of HAV for providing basis for prevention and controlling measures of human HA. Methods: Serum
HAV-IgG of swine in Dali was detected by enzyme-linked immunosorbent assay（ELISA）, and HAV-RNA of swine blood and swine
bile samples were tested by reverse transcription- polymerase chain reaction（RT-PCR）, with the blood and bile of HAV as positive
control. Results: ①HAV antibody positive rate of the blood samples of swine were detected as 73.3%（374/510）; ②RT-PCR method
was built to detect HAV; ③HAV-RNA of positive control samples were detected with RT-PCR; ④HAV-RNA of swine blood and
swine bile samples were not detected with RT-PCR. Conclusion: RT-PCR method is successfully built to detect HAV-RNA. The
antibody positive rate of HAV is rather high in the blood sample of swine. Further research is necessary to demonstrate whether the
swine could infect human HAV.

Key words: Hepatitis A virus（HAV）, swine, Enzyme-linked immunosorbent assay（ELISA）, Reverse transcription- Polymerase
chain reaction（RT-PCR）, Dali

CLC Number:

R512.91

Ai Zhiqiong, Zhang Ling, Li Lijuan, Li Ze, Wang Yunhong, Shen Yuanying. Establishment of Hepatitis A Virus RT-PCR Detection Method and
Its Epidemiological Investigation in Swinery[J]. J4, 2016, 1(10): 81-84.

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