Target Transformation Factor Analytical Method Used in the HPLC Peak-matching of the Gualou-Xiebai and the Herbs in Its Prescription

Abstract

Abstract: Objective: To investigate the principle and application of the asymptotic window target transformation factor analytical method in the HPLC chromatographic fingerprint peak matching. Methods: The fingerprints of the effective fraction in Gualou-Xiebai and the herbs in its prescription were obtained by using HPLC-PDAD, and the HPLC chromatographic peaks in fingerprints were peak-matched based on the principle of the asymptotic window target transformation factor analytical method. Results: With this method, there were 22 common peaks between the ethyl acetate extraction of Gualou-Xiebai and Gualou, and there were 10 common peaks between the n-butanol extraction of Gualou-Xiebai and Xiebai. Conclusion: The asymptotic window target transformation factor analytical method can match the chromatogram peaks quickly and accurately between different fingerprints.

Key words: asymptotic window target transformation factor analytical method, effective fraction in Gualou-Xiebai, herbsin prescription, chromato graphic fingerprint, peak-matching

CLC Number:

O657

Zou Chuncai, Yan Haiyan. Target Transformation Factor Analytical Method Used in the HPLC Peak-matching of the Gualou-Xiebai and the Herbs in Its Prescription[J]. , 2017, 2(6): 25-30.

References

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