Abstract:

Objective: To establish a method to determine the content of seven bioactive components in Forsythia suspensa. Methods: The chromatographic parameters of UPLC were optimized. A QMAS analytical method was built, with its linear range, recovery rate, precision, stability and repeatability evaluated. Results: The mobile phase consisted of 0.1% phosphoric acid solution andacetonitrile.Thedetectionwavelengthwas277nm.Theoptimumcolumntemperaturewas30℃and the optimum speed was0.3mL/min. Chlorogenic acid, caffeic acid, rutin, hyperoside, forsythoside A, phillyrin and arctigenin showed good linear relationships within the ranges of 0.009 80~0.196 00 μg（r=0.998 4）, 0.008 96~0.179 20 μg（r=0.998 9）, 0.008 00~0.240 00 μg（r=0.999 4）, 0.009 16~0.183 20 μg（r=0.999 2）, 0.020 72~0.414 40 μg（r=0.999 5）, 0.019 82~0.396 40 μg（r=0.999 1）, 0.006 96~0.139 20 μg（r=0.999 2）, and the average recoveries were 95.4%（RSD=3.78%）, 96.3%（RSD=4.61%）, 97.2%（RSD=4.04%）, 102.6%（RSD=3.91%）, 103.7%（RSD=2.37%）, 98.5%（RSD=1.99%）, 95.1%（RSD=4.58%）, respectively. Conclusion: A QMAS analytical method for seven bioactive components in Forsythia suspensa has been established, which has a high sensitivity and accuracy.

Key words: content determination, QAMS, Forsythia suspensa, UPLC, optimized chromatographic parameters