Abstract: Objective: To establish a HPLC method for the simultaneous determination of chlorogenic acid and baicalin in Yanfeishu
compound mixture and its quality control. Methods: The compound mixture was made from traditional Chinese medicine, such as Flos
Lonicerae, Forsythia suspensa, and Radix Scutellariae. HPLC was used. Mobile phase: methanol（B）-2% glacial acetic acid aqueous
（D）, column: Waters Symmetry -C18（5 μm, 4.6 mm× 150 mm）, linear gradient elution [0.0-7.5 min, B-D（20：80）→ B-D（70：
30）], flow rate: 1.00 mL/min, column temperature: 30 ℃. Using wavelength switching, the detection wavelength of chlorogenic acid was
327 nm, and the detection wavelength of baicalin was 274 nm. Results: The compound mixture of Yanfeishu was brown clear liquid.
The linear range of chlorogenic acid and baicalin were 0.019-0.750 μg/mL（r=0.999 9） and 0.032-1.280 μg/mL（r=0.999 9）,
respectively, and the average recovery rate（n=6）was 97.7%（RSD=1.69%）and 99.6%（RSD=0.88%）. Conclusion: The preparation
process of this preparation is simple and convenient. The method is proved to be simple, rapid, economical, reliable and suitable for the
quality control of compound mixture of Yanfeishu traditional Chinese medicine.

Key words: performance liquid chromatography, Yanfeishu compound mixture, chlorogenic acid, baicalin