Journal of Dali University ›› 2019, Vol. 4 ›› Issue (12): 69-74.

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Species Diversity and Biological Activity of Actinobacteria in Hehua Hot Spring of Yunnan Province

  

  1. (1. College of Public Health, Dali University, Dali, Yunnan 671000, China; 2. Institute of Eastern-Himalaya Biodiversity Research,
    Dali University, Dali, Yunnan 671003, China; 3. The Provincial Innovation Team of Biodiversity Conservation and Utility of the
    Three Parallel Rivers Region from Dali University, Dali, Yunnan 671003, China; 4. The Key Laboratory of Yunnan Education
    Department on Er'hai Catchment Conservation and Sustainable Development, Dali, Yunnan 671003, China; 5. Institute of
    Materia Medica, Dali University, Dali, Yunnan 671000, China; 6. College of Pharmacy and Chemistry,
    Dali University, Dali, Yunnan 671000, China)
  • Received:2019-04-11 Online:2019-12-15 Published:2019-12-15

Abstract: Objective: To explore the species diversity, enzymatic characteristic and antibacterial activity of actinomycetes in Hehua
Hot Spring and obtain some bioactive strains for further studies. Methods: The actinomycetes were isolated and purified by plate
dilution coating method and streak separation method, identified by morphological, physiological, biochemical and molecular biological
methods, and screened for enzymatic characteristic and antibacterial activity. Results: A total of 60 strains of actinomycetes were
isolated from Hehua Hot Spring, including of 51 strains of Streptomyces, 8 strains of Micromonospora, and 1 strain of Nomuraea. Among
these strains, 38.33% of the strains showed enzyme production characteristics, and 8 strains showed antibacterial activity against at
least one pathogen. Conclusion: Hehua Hot Spring is rich in actinomycetes, and many strains have good biological activity, which
deserves for further study.

Key words: hot spring, actinobacteria, species diversity, enzymatic characteristic, antibacterial activity