Abstract: Through the tissue culture of Rhododendron edgeworthii Hook. f. seedlings, the sterilization method and proliferation
culture formula of it were explored to establish the tissue culture system. The results shows that, the contamination rate of
Rhododendron edgeworthii Hook. f. was 7.5% when they were sterilized in 8 min treatment under 0.1% HgCl2; the bud induction
coefficient of Rhododendron edgeworthii Hook. f. was the highest, reaching 5.20, when the concentration of TDZ was 0.3 mg/L and the
concentration of IBA was 0.5 mg/L; the bud differentiation coefficient of Rhododendron edgeworthii Hook. f. was the highest, reaching
13.00, when the concentration of TDZ was 0.5 mg/L and the concentration of IBA was 0.05 mg/L; when the concentration of KT was
0.8 mg/L, the concentration of IBA was 0.08 mg/L, and the concentration of GA3 was 0.2 mg/L, the growth rate of the rhododendrons
was the highest, reaching 96.00%.

Key words: Rhododendron edgeworthii Hook. f. , disinfection time, callus induction, callus culture differentiation, tissue culture