Journal of Dali University ›› 2020, Vol. 5 ›› Issue (2): 31-37.DOI: 10. 3969 / j. issn. 2096-2266. 2020. 02. 008

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Correlation between Anti-inflammatory Activity and HPLC Spectrum of Different Polar Parts of#br# Geranium strictipes

L ü Xing 1 , Lu Jiansheng 1 , Geng Shuqiong 1 , Chen Junya 1 , Geng Ling 2 , Lan Hai 1 , Zhou Ping 1*   

  1. （1. College of Pharmacy and Chemistry, Dali University, Dali, Yunnnan 671000, China; 2. Pre-clinical College, Dali University, Dali,
    Yunnan 671000, China）
  • Received:2019-09-02 Revised:2019-11-13 Online:2020-02-15 Published:2020-02-15

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Abstract: Objective: To study the correlation among the spectra-effect relationship of HPLC spectrums of ethanol extracts and their
polar parts of Geranium strictipes and their anti-inflammatory activities in vivo, and explore the material basis of the anti-inflammatory
activity of Geranium strictipes. Methods: The HPLC spectrums of different parts of Geranium strictipes were established. The anti-
inflammatory activities of different parts of Geranium strictipes were determined by xylene-induced mouse ear swelling method. The
spectral-effect correlation between HPLC characteristic peaks of different parts and their anti-inflammatory effects was analyzed by
bivariate correlation analysis and partial least squares regression analysis. Results: Different parts of Geranium strictipes all exhibited
anti-inflammatory effects in which the ethyl acetate part worked best. The 27 characteristic chromatographic peaks in the HPLC
spectrums were selected, and 13 peaks were significantly correlated with the anti-inflammatory rate, the peaks ranked as No. 1（gallic
acid）, 2（methyl gallate）, 3（catechin）, 8, 11, 13, 15, 17, 19（pentagalloylglucose）, 20, 21, 25 and 27. Conclusion: The anti-
inflammatory activity of Geranium strictipes is the result of the coaction of multiple components.

Key words: Geranium strictipes, anti-inflammatory activity, HPLC spectrum, spectrum-effect relationship