Journal of Dali University ›› 2020, Vol. 5 ›› Issue (2): 56-60.DOI: 10. 3969 / j. issn. 2096-2266. 2020. 02. 012

Previous Articles     Next Articles

Influence of Xinmailong Injection on Microglia Activation and Subtype Differentiation after Cerebral#br# Ischemia/Reperfusion in Rats

Shen Lei 1, 2 , Wu Zhuo 2 , Liu Xiaoyi 3 , Li Ying 2 , Li Qiujing 2   

  1. （1.Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali, Yunnan 671000, China; 2. College of Pharmacy
    and Chemistry, Dali University, Dali, Yunnan 671000, China; 3. Clinical College, Dali University, Dali, Yunnan 671000, China）
  • Received:2019-04-15 Revised:2019-04-24 Online:2020-02-15 Published:2020-02-15

PDF (PC)

79

Abstract:  Objective: To study the influence of Xinmailong Injection（XML）on microglia activation and subtype differentiation after
middle cerebral artery occlusion/reperfusion（MCAO/R）in rats. Methods: After establishment of MCAO/R models, the medicines
were given after 1 h reperfusion, 3 times in total and 24 h interval each time. Then, the influences of XML on Iba-1+ cellular density in
ischemic penumbra were observed by immunofluorescence method, the effects of XML on the levels of CD16 and Arginase-1 gene
expression in ischemic penumbra were measured by Q-PCR. Results: Compared with MCAO/R model group, XML significantly
decreased activated microglia density in ischemic penumbra after MCAO/R in rats, reduced the levels of CD16 gene expression, while
could not affect Arginase-1 gene expression. Conclusion: XML reduced microglia activation in ischemic penumbra, decreased the
transformation of microglia to M 1 subtype, but not affect microglia transformation to M 2 subtype.

Key words:  Xinmailong Injection, cerebral ischemia/reperfusion, microglia, M 1 subtype, M 2 subtype