Abstract: Objective: To study the mechanism of Tongxie-Yaofang in treating inflammatory bowel disease using network pharmacology.
Methods: Effective chemical compositions and targets of drugs in Tongxie-Yaofang was searched in the Traditional Chinese Medicine
System Pharmacology Database and Analysis Platform（TCMSP）. Then the targets of IBD were obtained through GeneCards and
OMIM databases, the R software was used to find the common target and a "pharmaceutical component-disease target" network was
constructed by means of Cytoscape software. Finally, a common target protein interaction network was constructed through the STRING
website, core targets were screened out and the mechanism of its action was explored through GO analysis and KEGG pathway
enrichment analysis. Results: The study has found 39 effective pharmaceutical ingredients and 125 biological targets. Among the 104
targets that coincide with the disease, the core targets are IL6, AKT1, MAPK3, JUN, CASP3, and MAPK1, etc. The molecular functions
involved in GO analysis are mainly amide binding, nuclear receptor activity, transcription factor activity, phosphatase binding, and
steroid hormone receptor activity. KEGG pathway enrichment analysis is mainly for TNF signaling pathway, IL-17 signaling pathway,
Toll-like receptor signaling pathway etc. Conclusion: Tongxie-Yaofang is used to treat inflammatory bowel disease through "multicomponents,
multi-targets, and multi-pathways", which lays a foundation for the next step of experimental verification and new drug
development.

Key words: Tongxie-Yaofang, network pharmacology, inflammatory bowel disease, target, mechanism