Effects of Arsenic and Its Metabolites on cIAP1、cIAP2 and XIAP Genes in A549 Cells

doi:10. 3969/j. issn. 2096-2266. 2021. 10. 017

Abstract

Abstract:

Objective： To investigate the effects of arsenic and its metabolites on the expression of cIAP1、cIAP2 and XIAP in human lung aocarcinoma cell A549. Methods： A549 cells were exposed to different concentrations sodium arsenite （NaAsO2）， 60 ?滋mol/L monomethylarsonic acid （MMA） and dimethylarsinic acid （DMA） for 48 h， and the cell viability was detected with CCK-8 reagent. Based on the results of cell viability， A549 cells were poisoned with different concentrations of NaAsO2， 60 ?滋mol/L MMA and DMA， and Caspase-3 active reagent was used to detect the apoptosis and necrosis of cells after being poisoned. The mRNA expression levels of cIAP1， cIAP2 and XIAP were detected by real-time fluorescent quantitative polymerase chain reaction（PCR）. Results： With the increase of arsenic concentration， cell viability decreased. With the increase of dose， apoptosis and necrosis increased in NaAsO2 group， meanwhile MMA and DMA could also induce a small number of cell apoptosis. The results of real-time fluorescent quantitative PCR showed that the expression of cIAP1， cIAP2 and XIAP mRNA in the NaAsO2 exposure group and its metabolite MMA was significantly lower than that in the control group（P<0.05）； but DMA had no significant effect on the mRNA expression of these three genes. Conclusion：NaAsO2 and its metabolite MMA can reduce the expression of cIAP1， cIAP2 and XIAP mRNA in A549 cells， but its metabolite DMA had no significant effect on the mRNA expression of these three genes.

Key words:

"> arsenic">； MMA">； DMA">； apoptosis">；A549

CLC Number:

Chen Qian, Cheng Huirong, Huang Hao, Yang Jiao, Feng Wanjun, Wen Weihua.

Effects of Arsenic and Its Metabolites on cIAP1、cIAP2 and XIAP Genes in A549 Cells [J]. Journal of Dali University, 2021, 6(10): 76-.

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