Construction of fto Knockout Mouse Model
with CRISPR/Cas9 Gene Targeting Technology

Abstract

Abstract: Objective： This study aims to provides materials for FTO function research by constructing fto knockout mouse model with CRISPR/Cas9 gene targeting technology. Methods： Cas9 mRNA and guide RNA（gRNA） targeting fto were microinjected into mouse fertilized eggs in vitro， and the knockout of mouse fto gene was identified and verified by ploymerase chain reaction（PCR）， PCR product sequencing and Western-Blotting. Results： The active gRNA vector and Cas9 mRNA were injected into the fertilized egg successfully in vitro， and three positive F0 mice were obtained at last. The homozygous mice with fto gene knockout were finally obtained by hybridization and mating with the same mice. Conclusion： The fto gene knockout mouse model through CRISPR/Cas9 technology was successfully prepared， which laid a foundation for further research on the function of fto gene.

Key words: font-family:", ">"> , ">ftofont-family:Times New Roman, ">；font-family:Times New Roman, "> obesityfont-family:Times New Roman, ">；font-family:Times New Roman, "> knockout mousefont-family:Times New Roman, ">；font-family:Times New Roman, "> CRISPR/Cas9

CLC Number:

Hua Hui, Xia Jing, Jia Huijie, Ge Yuchen, Wang Xiaobo .

Construction of fto Knockout Mouse Model with CRISPR/Cas9 Gene Targeting Technology [J]. Journal of Dali University, 2022, 7(2): 33-38.

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Construction of fto Knockout Mouse Model with CRISPR/Cas9 Gene Targeting Technology

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