Journal of Dali University ›› 2022, Vol. 7 ›› Issue (6): 91-96.

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Identification and Antibacterial Activity Study of Four Streptomyces Strains

  

  1. 1. Institute of Eastern-Himalaya Biodiversity Research, Dali University, Dali, Yunnan 671003, China; 2. College of Agriculture and Biology Science, Dali University, Dali, Yunnan 671003, China;3. College of Public Health, Dali University, Dali, Yunnan 671000, China
  • Received:2021-05-06 Revised:2021-06-04 Online:2022-06-15 Published:2022-07-04
  • Supported by:

    国家自然科学基金项目(31360013);第二次青藏高原综合科学考察研究项目(2019QZKK0402

Abstract:

Objective To identify the actinomycete strains separated from soil and study their antibacterial activity. Methods Streak method was used to separate and purify four strains of StreptomycesDL002 DL003 DL004 DL005), and morphological and molecular biological methods were used to identify these strains. After that plate confrontations and filtering paper methods were used to study the antibacterial activity of them against standard strains of bacteria and fungi. Results The tested strains were Streptomyces flavogriseus. The growth of Bacillus subtilis Proteus vulgarisEscherichia coli Pseudomonas aeruginosa Aspergillus niger Rhizoctonia solani and the spore production of A. niger can be inhibited by any of the four strains. Among the four stains strain DL002 had the strongest inhibition effecton P. vulgaris and B. subtilis. Conclusion The tested strains all have inhibition effect on bacteria and fungi, especially, strain DL002 showed the strongest antibacterial activity and the ability to inhibit A.niger spore production, which could be further studied.

Key words:

Streptomyces flavogriseus, antibacterial activity, Aspergillus niger, Proteus vulgaris, Bacillus subtilis

CLC Number: