Journal of Dali University ›› 2023, Vol. 8 ›› Issue (10): 27-33.

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Research on the Application of Gold Nanorods in the Detection of Circulating Antigens in Clonorchis sinensis Serum

Chen Liping1， Lian Xiaoli2， Cai Zihan1， Li Qian3， Ouyang Tianzhao4， Yang Yimei1*   

  1. （1. Pre-clinical College， Dali University， Dali， Yunnan 671000， China； 2. Jinci College， Shanxi Medical University， Taiyuan， 030000， China； 3. Clinical College， Dali University， Dali， Yunnan 671000， China； 4. The First Affiliated Hospital of Dali University， Dali，Yunnan 671000， China）

  • Received:2022-12-06 Revised:2022-12-23 Online:2023-10-15 Published:2023-10-26

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Abstract:

Objective： To study the detection effect of gold nanorods on early and low worm burden of Clonorchis sinensis. Methods： Gold nanorods were labeled with different concentrations of thiolated Clonorchis sinensis-specific IgG antibodies. The absorption peaks of the gold nanorods were scanned using a UV spectrophotometer to observe the magnitude of the peak shift and determine the optimal labeling concentration. The gold nanorods labeled with the optimal concentration of antibodies were used to detect circulating antigens in rat serum with different infection degrees， infection times， and dilutions.  Results： The maximum peak shift was 20.0 nm when the gold nanorods were labeled with 20 μg/mL antibodies. The gold nanorods labeled with Clonorchis sinensis specific IgG antibodies could detect circulating antigens in rat serum at 3， 10 ，17 and 31 d after infection with 50， 100 and 200 cercariae， as well as at a dilution of 1∶400. Conclusion： Gold nanorods labeled with Clonorchis sinensis specific IgG antibodies have significant advantages in the detection of early and low worm burden of Clonorchis sinensis.

Key words:

gold nanorods, Clonorchis sinensis, circulating antigen, specific IgG antibodies, detection

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