Effects of Intestinal Flora and Serum Metabolites on Visceral Sensitivity in Post-Infection Irritable Bowel Syndrome

Abstract

Abstract: Objective：To investigate the changes of intestinal flora and serum metabolites in mice with post-infection irritable bowel
syndrome （PI-IBS）, and analyze the effects on visceral sensitivity. Methods：The PI-IBS model was prepared by Trichinella spiralis
infection method, and the intestinal microbial changes of PI-IBS mice and normal mice were analyzed by 16S rDNA sequencing. The
changes of serum metabolites were analyzed by serum metabolomics. Spearman's correlation analysis was used to analyze the
correlation between different metabolites and different flora in the two groups. Results：The results of 16S rDNA sequencing showed
that mouse gut metagenome and g_Alistipes were the critical gut microbes in PI-IBS mice. A total of 22 intergroup differential
metabolites were identified by metabolomics analysis, involving 16 metabolic pathways, mainly including biosynthesis of amino acids,
cysteine and methionine metabolism, arginine biosynthesis, synaptic vesicle cycle and other pathways. Conclusion：Changes in
intestinal flora and serum metabolites are involved in the production of visceral hypersensitivity to PI-IBS.

Key words: post-infection irritable bowel syndrome, 16S rDNA sequencing, intestinal flora, metabolomics

CLC Number:

R574.4

Yan Bo, Pan Ying, Tian Pingping. Effects of Intestinal Flora and Serum Metabolites on Visceral Sensitivity in Post-Infection Irritable Bowel Syndrome[J]. Journal of Dali University, 2024, 9(2): 24-31.

References

〔1〕 FORD A C，SPERBER A D，CORSETTI M，et al. Irritable
Bowel Syndrome〔J〕. Lancet，2020，396（10263）：1675-
1688.
〔2〕肖红云，李欢，闫波，等. APETx2对感染后肠易激综合征
小鼠内脏敏感性的作用及机制〔J〕. 安徽医科大学学报，
2023，58（6）：953-958.
〔3〕耿岚岚，刘明南，龙高，等. 儿童功能性胃肠病罗马Ⅳ标
准〔J〕. 中华儿科杂志，2017，55（1）：4-7.
〔4〕 YUAN X M，CHEN B Q，DUAN Z L，et al. Depression and
Anxiety in Patients with Active Ulcerative Colitis：Cross⁃
talk of Gut Microbiota，Metabolomics and Proteomics〔J〕.
Gut Microbes，2021，13（1）：1987779.
〔5〕 LEUNG H，LONG X X，NI Y Q，et al. Risk Assessment
with Gut Microbiome and Metabolite Markers in NAFLD
Developmen〔t J〕. Sci Transl Med，2022，14（648）：eabk0855.
〔6〕杨波，蓝程，周旭春. 感染后肠易激综合征小鼠Cajal间
质细胞改变对肠道动力和内脏敏感性的影响〔J〕. 上海
交通大学学报（医学版），2014，34（7）：978-983.
〔7〕 YUAN L P，XIAO H Y，LI H，et al. APETx2 Regulates In⁃
testinal Motility and Visceral Sensitivity in Post-Infectious
Irritable Bowel Syndrome Mice through 5-HT Signalling
Pathway〔J〕. J Pharm Pharmacol，2023，75（5）：712-717.
〔8〕 LAN C，SUN X N，ZHOU X C，et al. Preinduced Intestinal
HSP70 Improves Visceral Hypersensitivity and Abnormal
Intestinal Motility in PI-IBS Mouse Model〔J〕. Asian Pac J
Trop Med，2016，9（3）：302-305.
〔9〕 QIN H Y，WU J C Y，TONG X D，et al. Systematic Review
of Animal Models of Post-Infectious/Post-Inflammatory Ir⁃
ritable Bowel Syndrome〔J〕. J Gastroenterol，2011，46（2）：
164-174.
〔10〕 JALANKA J，SALONEN A，FUENTES S，et al. Microbial
Signatures in Post-Infectious Irritable Bowel Syndrome：
Toward Patient Stratification for Improved Diagnostics
and Treatmen〔t J〕. Gut Microbes，2015，6（6）：364-369.
〔11〕 CHENG F L，FAN Z Z，LIN C H，et al. Effect of Altered
Gut Microbiota on Visceral Hypersensitivity of Postinfec⁃
tious Irritable Bowel Syndrome Mice〔J〕. Eur J Gastroen⁃
terol Hepatol，2022，34（12）：1220-1230.
〔12〕 PARKER B J，WEARSCH P A，VELOO A C M，et al. The
Genus Alistipes：Gut Bacteria with Emerging Implications
to Inflammation，Cancer，and Mental Health〔J〕. Front Im⁃
munol，2020，11：906.
〔13〕 SONG Y L，KÖNÖNEN E，RAUTIO M，et al. Alistipes on⁃
derdonkii Sp. Nov. and Alistipes shahii Sp. Nov. of Human
Origin〔J〕. Int J Syst Evol Microbiol，2006，56（Pt8）：
1985-1990.
〔14〕肖红云，李欢，闫波，等. APETx2对感染后肠易激综合
征小鼠内脏敏感性的作用及机制〔J〕. 安徽医科大学学
报，2023，58（6）：953-958.
〔15〕 SAULNIER D M，RIEHLE K，MISTRETTA T，et al. Gas⁃
trointestinal Microbiome Signatures of Pediatric Patients
with Irritable Bowel Syndrome〔J〕. Gastroenterology，2011，
141（5）：1782-1791.
〔16〕 BAIER J L，GÄNSBAUER M，GIESSLER C，et al. Argi⁃
nase Impedes the Resolution of Colitis by Altering the Mi⁃
crobiome and Metabolome〔J〕. J Clin Invest，2020，130
（11）：5703-5720.
〔17〕 KAO C C，COPE J L，HSU J W，et al. The Microbiome，
Intestinal Function，and Arginine Metabolism of Healthy
Indian Women Are Different from Those of American and
Jamaican Women〔J〕. J Nutr，2015，146（4）：706-713.
〔18〕 CRENN P，HANACHI M，NEVEUX N，et al. Circulating

Citrulline Levels：A Biomarker for Intestinal Functionality

Assessment〔J〕. Ann Biol Clin （Paris），2011，69（5）：
513-521.
〔19〕 GRIMBLE R F. The Effects of Sulfur Amino Acid Intake
on Immune Function in Humans〔J〕. J Nutr，2006，136（6
Suppl）：1660S-1665S.
〔20〕 BROSNAN J T，BROSNAN M E. The Sulfur-Containing
Amino Acids：An Overview〔J〕. J Nutr，2006，136（6 Suppl）：
1636S-1640S.
〔21〕 NI J，SHEN T C D，CHEN E Z，et al. A Role for Bacterial
Urease in Gut Dysbiosis and Crohn's Disease〔J〕. Sci
Transl Med，2017，9（416）：eaah6888.
〔22〕 NG S Y，ONG K X，SURENDRAN S T，et al. Hydrogen
Sulfide Sensitizes Acinetobacter baumannii to Killing by
Antibiotics〔J〕. Front Microbiol，2020，11：1875.
〔23〕WALKER A，SCHMITT-KOPPLIN P. The Role of Fecal
Sulfur Metabolome in Inflammatory Bowel Diseases〔J〕.
Int J Med Microbiol，2021，311（5）：151513.
〔24〕 LIN M J，YU B P. Upregulation of the High-Affinity Cho⁃
line Transporter in Colon Relieves Stress-Induced Hyper⁃
algesia〔J〕. J Pain Res，2018，11：1971-1982.
〔25〕 LONG Y Q，LIU Y，TONG J J，et al. Effectiveness of
Trimebutine Maleate on Modulating Intestinal Hypercon⁃
tractility in a Mouse Model of Postinfectious Irritable
Bowel Syndrome〔J〕. Eur J Pharmacol，2010，636（1/2/3）：
159-165.

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