Journal of Dali University ›› 2025, Vol. 10 ›› Issue (8): 41-50.DOI: 10. 3969 / j. issn. 2096-2266. 2025. 08. 006

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The Mechanism of LINC00460-miR143-COL1A1 Expression Regulation in the Proliferation, Migration,#br# and Invasion of Colorectal Cancer Cells

Qi Xiaopeng, Cheng Wei, Tian Chang, Tao Lun, Luo Xiaohong, Shu Wuying*   

  1. (Department of General Surgery, The First Affiliated Hospital of Dali University, Dali, Yunnan 671000, China)
  • Received:2024-01-25 Revised:2024-04-22 Online:2025-08-15 Published:2025-09-06

Abstract: Objective: To investigate the role of LINC00460 in the mechanism of colorectal cancer and its impact on cell proliferation,
migration, and invasion capabilities. Methods: The competitive endogenous RNA regulatory network of lncRNA-miRNA-mRNA in
colorectal cancer patient tissue samples from the TCGA database was analyzed. In vitro experimental studies were conducted using
cell culture, lentiviral transfection, CCK-8 kit detection, scratch assay, Transwell invasion assay, as well as RT-PCR and qPCR
techniques. Results: LINC00460 was significantly upregulated in colorectal cancer tissues, with its expression negatively correlated
with miR143 and positively correlated with COL1A1 mRNA. Silencing LINC00460 expression significantly decreased the
proliferation, migration, and invasion of colorectal cancer cells, whereas LINC00460 overexpression significantly enhanced the
proliferation, migration, and invasion of normal colonic epithelial cells. Conclusion: LINC00460 may promote the proliferation,
migration, and invasion of colorectal cancer cells by competitively inhibiting miR143 (as a target) and upregulating COL1A1 mRNA
expression, and thus may serve as a potential therapeutic target for colorectal cancer.

Key words: colorectal cancer, LINC00460, miR143, COL1A1 mRNA

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