Table of Content

15 August 2025, Volume 10 Issue 8

Previous Issue   

Changes in Color and Chemical Components during the Aging Process of Artemisia argyi Leaves

Yang Dandan, Huang Haozhou, , Bao Xiaoming, Yang Ming, Zhang Dingkun,

2025, 10(8):  1-10.  DOI: 10. 3969 / j. issn. 2096-2266. 2025. 08. 001

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Objective: To investigate the dynamic changes in color, volatile and non-volatile components of Artemisia argyi leaves
during natural aging, and clarify the mechanism of chemical component transformation, providing a scientific basis for quality
evaluation and optimization of the aging process. Methods: A. argyi leaves of different aging years were used as the research object.
The color change was characterized by measuring the color parameters using a colorimeter. The volatile oil of A. argyi leaves was
extracted by steam distillation and the yield was calculated.Gas chromatogram-mass spectrometry （GC-MS） combined with orthogonal
partial least squares discriminant analysis （OPLS-DA） was applied to analyze the composition and differences of volatile components.
High-performance liquid chromatography （HPLC） was used to quantify five non-volatile components, including neochlorogenic acid
and chlorogenic acid. Results: With the prolongation of aging time, the color of A. argyi leaves gradually shifted from green to
yellowish-brown, and the volatile oil yield significantly decreased. GC-MS identified 92 volatile components, among which alkenes
and alcohols were the major components. OPLS-DA screened out 20 differential components, with contents of borneol, methyl eugenol,
p-cymen-8-ol, dihydrocarvone, and α-thujone increasing significantly. For non-volatile components, phenolic acids degraded faster
than flavonoids. Conclusion: The aging of A. argyi leaves is driven by the synergistic effects of volatile component transformation, nonvolatile
component degradation, and physicochemical changes. The formation of compounds like borneol reveals the complexity of
chemical conversion, while color changes serve as a visual indicator of aging progression. These findings provide a scientific basis for
establishing a quality evaluation system and optimizing the aging process, and offer a modern scientific interpretation of the traditional
concept "the longer the aging, the better the efficacy".

Formulation Optimization and Wound Healing Effect of a Periplaneta americana Composite#br# Spray Film in Rats

Zhang Jingyu, , Chen Zhongze, Li Kailing, He Rongmao, Zhao Zhitao, Yang Yongshou, Xiao Peiyun

2025, 10(8):  11-19.  DOI: 10. 3969 / j. issn. 2096-2266. 2025. 08. 002

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Objective: To prepare a Periplaneta americana composite spray film, optimize its formulation, and assess its efficacy in
wound healing of rats. Methods: Single-factor experiments were conducted to screen film-forming excipients based on evaluation
indices including spray atomization performance, film-forming time, moisturizing capacity, and mechanical properties of the film. The
formulation ratios were further optimized by response surface methodology. A rat skin wound model was established to evaluate the
wound healing effect of the optimized spray film. Results: The optimal film-forming excipients were determined to be Bletilla striata
polysaccharide （BSP）, polyvinylpyrrolidone K30 （PVPK30）, and poloxamer 188 （P188）, with glycerol as the plasticizer and benzoic
acid as the preservative. The optimized formulation comprised 5.0% PVPK30, 2.0% P188, 0.7% BSP, 3.0% P. americana extract, 4.0%
glycerol, and 0.1% benzoic acid. The wound healing study showed that the spray film significantly promoted wound closure and healing
in rats. Conclusion: The optimized P. americana composite spray film demonstrated stable preparation technology, excellent filmforming
ability, and significant promoting effects on acute skin wound healing in rats.

Evaluation and Validation of Cumulative Release Rate of Transdermal Patches Containing Cassia Seed#br# Extract Using Cumulative Fingerprint of Traditional Chinese Medicine

Yan Haiyan, Huang Li, Zou Chuncai

2025, 10(8):  20-29.  DOI: 10. 3969 / j. issn. 2096-2266. 2025. 08. 003

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Objective: To evaluate and validate the cumulative release rate of transdermal patches containing Cassia seed extract
using cumulative traditional Chinese medicine fingerprint（TCM fingerprint）. Methods: Based on the theory in Fingerprint of
Traditional Chinese Medicine and Its Digitization, the concept, principle, and calculation formula of cumulative TCM fingerprint were
established. Taking the transdermal cumulative release rate of transdermal patches containing Cassia seed extract as an example, the
results were compared with the data processing outcomes of dissolution rate and release tests rate specified under item 0931 in General
Chapter of Chinese Pharmacopoeia （ChP） 2020 Edition. Results: The cumulative release rate of components in transdermal patches
containing Cassia seed extract varied at 24 hours: 14 components exceeded 80.0%, while 1 component was below 10.0%. When
comparing cumulative TCM fingerprint with the cumulative release rate results at different sampling time points specified in ChP 2020,
the absolute values of the mean error were all below 2.3%. Conclusion: The cumulative TCM fingerprint can be applied to evaluate the
cumulative release rate of TCM preparations, with accurate results and simple operation, offering a new approach for quality control and
inspection of TCM preparations.

Isolation and Identification of the Pathogen of Stem Rot in Dendrobium devonianum

Li Chaohai, , , Long Ruimin, , Liang Liyan, , Zhou Ying, , , Li Nengbo, Liu Weihong, Li Xinping, , Duan Baozhong,

2025, 10(8):  30-35.  DOI: 10. 3969 / j. issn. 2096-2266. 2025. 08. 004

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Objective: To isolate and identify the pathogen of stem rot in Dendrobium devonianum. Methods: Diseased tissues of D.
devonianum were collected, and the pathogen was isolated and purified using tissue isolation method. Colony morphological
characteristics were observed, Koch's postulates were fulfilled to verify pathogenicity, and the pathogen was identified based on
morphological characteristics combined with phylogenetic tree constructed using rDNA-ITS sequences. Results: Typical symptoms of
stem rot in D. devonianum included water-soaked spots, leaf chlorosis, wilting, and defoliation, ultimately leading to plant death. The
isolated pathogen was identified as Fusarium proliferatum, and the symptoms of artificially inoculated plants were consistent with those
observed in the field. Morphological and molecular analyses showed that strain ZB-84 was highly consistent with F. proliferatum, and
phylogenetic analysis supported its close genetic relationship with this species. Conclusion: This is the first report identifying F.
proliferatum as the pathogen of stem rot in D. devonianum. The findings provide new insights for the development of stem rot control
strategies, and lay a foundation for further studies on the pathogenic mechanism of the pathogen and the development of specific control
technologies.

The Photodynamic Antibacterial Properties and Mechanism of Zn/g-C3N4 Nanomaterials

Fu Niao, Wang Xia, Liu Yujiao, He Jieli

2025, 10(8):  36-40.  DOI: 10. 3969 / j. issn. 2096-2266. 2025. 08. 005

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Objective: To construct an efficient photodynamic antibacterial system, this study systematically investigates the
photodynamic antibacterial properties and mechanism of Zn/g-C3N4 nanomaterials. Methods: Zn/g-C3N4 nanomaterials with Zn doping
ratios of 1%, 5%, 10%, 15%, and 20% were prepared by a calcination method. Escherichia coli （E. coli） was selected as the model
strain. The antibacterial efficacy against E. coli was assessed via the plate spreading method. The photodynamic antibacterial
mechanism was systematically studied through reactive oxygen species quenching assays and analysis of cellular component leakage.
Results: The antibacterial activity exhibited a trend of first increasing and then decreasing with the elevation of Zn doping ratio. The
10% Zn/g-C3N4 nanomaterial exhibited optimal antibacterial performance, achieving complete inhibition of E. coli within 60 minutes.
Quenching experiments identified hydroxyl radicals, superoxide anions, and singlet oxygen as the primary reactive oxygen species.
Further investigation revealed that photodynamic treatment led to substantial glutathione depletion and significant leakage of
intracellular nucleic acids and proteins in bacteria. Conclusion: Under visible light irradiation, 10% Zn/g-C3N4 nanomaterials
efficiently generate reactive oxygen species, which mediate a cascade antibacterial mechanism involving "reactive oxygen species
attack-antioxidant defense collapse-membrane structure damage-intracellular content leakage". This study provides theoretical basis
and experimental support for the design and application of novel photosensitizing antibacterial materials based on Zn/g-C3N4.

The Mechanism of LINC00460-miR143-COL1A1 Expression Regulation in the Proliferation, Migration,#br# and Invasion of Colorectal Cancer Cells

Qi Xiaopeng, Cheng Wei, Tian Chang, Tao Lun, Luo Xiaohong, Shu Wuying

2025, 10(8):  41-50.  DOI: 10. 3969 / j. issn. 2096-2266. 2025. 08. 006

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Objective: To investigate the role of LINC00460 in the mechanism of colorectal cancer and its impact on cell proliferation,
migration, and invasion capabilities. Methods: The competitive endogenous RNA regulatory network of lncRNA-miRNA-mRNA in
colorectal cancer patient tissue samples from the TCGA database was analyzed. In vitro experimental studies were conducted using
cell culture, lentiviral transfection, CCK-8 kit detection, scratch assay, Transwell invasion assay, as well as RT-PCR and qPCR
techniques. Results: LINC00460 was significantly upregulated in colorectal cancer tissues, with its expression negatively correlated
with miR143 and positively correlated with COL1A1 mRNA. Silencing LINC00460 expression significantly decreased the
proliferation, migration, and invasion of colorectal cancer cells, whereas LINC00460 overexpression significantly enhanced the
proliferation, migration, and invasion of normal colonic epithelial cells. Conclusion: LINC00460 may promote the proliferation,
migration, and invasion of colorectal cancer cells by competitively inhibiting miR143 （as a target） and upregulating COL1A1 mRNA
expression, and thus may serve as a potential therapeutic target for colorectal cancer.

The Function of FIBCD1 in Proliferation of Human Lung Cancer Cell Line A549

Li Hua, Zhang Min, Fang Peifei, Zhang Yanfei

2025, 10(8):  51-56.  DOI: 10. 3969 / j. issn. 2096-2266. 2025. 08. 007

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Objective: To explore the role of fibrinogen-related C domain 1 （FIBCD1） in lung cancer. Methods: A FIBCD1-
knockdown A549 lung cancer cell line was constructed using lentivirus-mediated RNA interference. The knockdown efficiency was
validated by quantitative real-time PCR and Western blotting. Cell proliferation was assessed by MTT assay, while cell cycle
distribution and apoptosis were analyzed via flow cytometry. The effects of FIBCD1 knockdown on colony formation and tumor growth
were evaluated using colony formation assay and in vivo xenograft tumor formation assay in nude mice. Results: FIBCD1 knockdown
significantly inhibited A549 cell proliferation, induced G0/G1 phase cell cycle arrest, and markedly increased the apoptotic rate.
Colony formation assay further confirmed that FIBCD1 suppression impaired the colony-forming ability of A549 cells. In vivo
experiments showed that FIBCD1 knockdown significantly attenuated tumor growth in nude mice, with reduced tumor weight and in
vivo luminescence intensity compared to the control group. Conclusion: FIBCD1 exerts a role in promoting proliferation and inhibiting
apoptosis in lung cancer cells. Its high expression may be closely associated with tumor growth. Suppression of FIBCD1 significantly
reduces the tumorigenic potential of lung cancer cells, indicating that FIBCD1 holds promise as a potential therapeutic target for lung
cancer.

The Effect of Regorafenib Combined with PD-1 Inhibitor on Survival Rates, Disease-Related Factors,#br# and Adverse Reactions in Patients with Advanced Rectal Cancer

Zhang Lu, Han Zhengquan, Zhang Haibin, Gao Zhenyuan

2025, 10(8):  57-61.  DOI: 10. 3969 / j. issn. 2096-2266. 2025. 08. 008

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Objective: To evaluate the effect of regorafenib in combination with PD-1 inhibitor on survival rates, levels of diseaserelated
factors, and adverse reactions in patients with advanced rectal cancer. Methods: Eighty patients with advanced rectal cancer
admitted to the First Affiliated Hospital of Bengbu Medical University from January 2019 to December 2020 were selected and divided
into the experimental group （regorafenib combined with PD-1 inhibitor, 40 cases） and the control group （regorafenib monotherapy, 40
cases） by the random number table method. The primary observed indicators include treatment efficacy, levels of disease-related factors
（fatty acid synthetase （FAS）, tumor M2-type pyruvate kinase （TuM2-PK）, epidermal growth factor （EGF）, interleukin-17 （IL-17）,
insulin-like growth factor 1 （IGF-1）, tumor necrosis factor α （TNF-α））, survival rates, and incidence of adverse reactions. Results:
The objective response rate of patients in the experimental group was significantly higher than that in the control group （P<0.05）. After
treatment, the levels of FAS, TuM2-PK, EGF, IL-17, IGF-1 and TNF-α in both groups were significantly decreased（ P<0.05）, and the
levels of various disease-related factors in the experimental group were significantly higher than those in the control group （P<0.05）.
The survival rates of patients in the experimental group at 1 year and 2 years after treatment were significantly higher than those in the
control group （P<0.05）, and the 3-year survival rate was higher than that in the control group, but the difference was not statistically
significant（ P>0.05）. The main adverse reactions of patients in both groups included gastrointestinal symptoms, abnormal liver function,
abnormal renal function, skin reactions and bleeding risk. There was no statistically significant difference in the incidence of each
adverse reaction （P>0.05）. Conclusion: Regorafenib in combination with PD-1 inhibitor significantly improves the objective response
rate and survival rates, reduces levels of disease-related factors, and does not increase the incidence of adverse reactions in patients
with advanced rectal cancer, showing good clinical application prospects.

Application of Intranasal Dexmedetomidine in General Anesthesia for Elderly Patients

Yuan Jianchun, Cao Bo, Su Lihong, Zhou Yun

2025, 10(8):  62-66.  DOI: 10. 3969 / j. issn. 2096-2266. 2025. 08. 009

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Objective: To explore the application of intranasal dexmedetomidine at different doses in elderly patients during general
anesthesia. Methods: A total of 60 elderly patients undergoing elective surgery under general anesthesia at People's Hospital of Yuxi
City from January to September 2023 were randomly divided into three groups: Group A（ 0.5 μg/kg）, Group B（ 0.8 μg/kg）, and Group C
（1.0 μg/kg）, with 20 patients in each group. Intranasal dexmedetomidine was administered 30 minutes prior to anesthesia induction in all
groups. Mean arterial pressure （MAP）, heart rate （HR）, and rate pressure product （RPP） were measured at different time points.
Awakening time, extubation time, postanesthesia care unit （PACU） stay duration, extubation quality scores, Ramsay sedation scores,
and incidences of bradycardia were also compared. Results: In Group A, MAP, HR, and RPP were significantly increased during
induction intubation and tracheal extubation, with adverse reactions such as coughing during extubation. Groups B and C exhibited more
stable vital signs and higher extubation quality scores. Notably, the Ramsay sedation score in Group C was significantly higher than in
Groups A and B, but Group C also showed postoperative drowsiness and prolonged PACU stay. Conclusion: Administering intranasal
dexmedetomidine at 0.8 μg/kg 30 minutes prior to anesthesia induction effectively suppresses stress responses during intubation and
extubation in elderly patients undergoing elective general anesthesia. This dose demonstrates minimal adverse effects and is suitable
for clinical application in anesthesia management.

Research on the Current Status and Influencing Factors of the Geriatric Health Management Service#br# Capacity of Primary Care Physicians in Yuxi City

Li Yajie, Li Ji, Yang Yudie, Zhang Di, Ma Zhenfei, Wang Yanbo, Qi Kun

2025, 10(8):  67-72.  DOI: 10. 3969 / j. issn. 2096-2266. 2025. 08. 010

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Objective: To analyze the current status and influencing factors of geriatric health management service capacity of
primary care physicians in Yuxi City, and to provide decision-making references for improving their geriatric health management
service capacity. Methods: A self-designed questionnaire was used to survey primary care physicians in Yuxi City. Binary Logistic
regression analysis was applied to identify factors influencing their geriatric health management service capacity. Results: A total of
1 450 physicians（ accounting for 82.48%） possessed five or more geriatric health management service capabilities. Physicians over 45
years old had significantly higher rates of mastering various capabilities than those under 30 years old（ P<0.016 7）. Rural primary care
physicians had a higher rate of mastering hypertension and diabetes diagnosis and treatment capabilities than their urban counterparts
（P<0.05）. The most deficient capabilities among primary care physicians were diagnosis and treatment of hypertension and diabetes,
and handling of cardiovascular and cerebrovascular emergencies. Educational level, professional qualification, and practice setting
were identified as factors influencing their geriatric health management service capacity （P<0.05）. Conclusion: Primary care
institutions in Yuxi City face talent shortages and an aging workforce. Capabilities in chronic disease management and handling
cardiovascular and cerebrovascular emergencies require improvement. Guided by policies, efforts should be made to expand
development prospects for primary care physicians, focus on training rural doctors, and comprehensively enhance their geriatric health
management capacity.

Awareness, Acceptance, and Influencing Factors of HPV and HPV Vaccines among Female College#br# Students in the Dali Region

Li Zhonglian, , Yang Qiongyan, Luo Ruishi, Zhang Zixia, Li Wenyin, Guo Ying

2025, 10(8):  73-79.  DOI: 10. 3969 / j. issn. 2096-2266. 2025. 08. 011

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Objective: To assess the awareness and acceptance of human papilloma virus （HPV） and HPV vaccines among female
college students in the Dali region and to analyze the factors influencing these aspects, with the goal of providing a basis for increasing
HPV vaccination rates. Methods: A total of 1 000 female college students from two universities in Dali City were selected using
random sampling. A questionnaire survey was administered to collect data on their knowledge of and acceptance toward HPV and HPV
vaccines. Data were analyzed using SPSS 25.0 software, and relevant influencing factors were explored. Results: The awareness rate of
HPV among female college students in Dali City was 67.97%, and that of HPV vaccines was 66.43%; the vaccination rate was 31.62%.
HPV and HPV vaccines awareness significantly differed by grade, academic major, contraceptive use, and sexual experience. Moreover,
high vaccine cost and insufficient knowledge of the reservation process were the primary reasons for the low vaccination rate.
Conclusion: Female college students in the Dali region have relatively low awareness of HPV and HPV vaccines. It is recommended to
improve vaccination rates by strengthening health education, optimizing the reservation process, and reducing vaccine costs, thereby
effectively lowering the incidence of cervical cancer.

Analysis of the Awareness of Core Knowledge of Chronic Diseases and Its Influencing Factors among#br# Permanent Residents in Yuxi City

Li Ji, Yang Yudie, Qi Kun, Zhang Di, Li Yajie, Ma Zhenfei, Wang Yangbo

2025, 10(8):  80-85.  DOI: 10. 3969 / j. issn. 2096-2266. 2025. 08. 012

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Objective: To investigate the awareness of core knowledge of chronic diseases among permanent residents in Yuxi City
and its influencing factors, and to provide a scientific basis for the formulation of chronic disease prevention and control policies and
intervention measures in Yuxi City. Methods: A multistage stratified random sampling method was adopted to select permanent
residents aged 18 years and above from each county （city, district） of Yuxi City for a questionnaire survey on core knowledge of
chronic diseases. The awareness rate of core knowledge was calculated, and univariate and multivariate Logistic regression models were
used to analyze factors influencing the awareness rate. Results: The awareness rate of core knowledge of chronic diseases in Yuxi City
was 79.95%. Multivariate Logistic regression analysis showed that the awareness rate was higher among Han people, those with higher
educational levels, clerical staff, and professional technicians （P<0.05）. Conclusion: The popularization of core knowledge of chronic
diseases among residents in Yuxi City has achieved certain results and met established requirements. However, there are differences in
awareness rates among people with different characteristics. Targeted health education should be provided to ethnic minorities,
individuals with low educational levels, and workers employed in agriculture, forestry, animal husbandry, fishery, and water
conservancy to comprehensively enhance residents' awareness rate of core knowledge of chronic diseases.

Effect of Group Counseling Application on Transition Shock among Intern Nursing Students in Infectious#br# Disease Hospitals

Zhou Wenxian, Zhao Na, Yang Xuan, He Qilian, Zhang Xiong, Wu Kunli

2025, 10(8):  86-92.  DOI: 10. 3969 / j. issn. 2096-2266. 2025. 08. 013

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Objective: To explore the effect of group counseling in reducing transition shock among intern nursing students in
infectious disease hospitals. Methods: Intern nursing students undergoing clinical practice at a tertiary infectious disease hospital in
Kunming from June 2023 to April 2024 were selected and randomized into an experimental group and a control group using a random
number table, with 43 students in each group. The control group only attended routine pre-employment training organized by the
hospital, while the experimental group received 8-week group counseling （once weekly, 120 minutes per session） in addition to the
routine training. The Chinese version of the Nursing Undergraduate Transition Shock Assessment Scale was used to assess transition
shock levels in the two groups before intervention, at 4 weeks post-intervention, 8 weeks post-intervention, and 3 months after
intervention completion. Results: After 4 weeks of intervention, there was no statistically significant difference in the total score of
transition shock between the two groups of intern nursing students （P=0.160）. However, after 8 weeks of intervention and 3 months
after the end of the intervention, there was a statistically significant difference in the total score of transition shock between the two
groups of intern nursing students （P<0.001）. The time effect, group effect, and interaction effect of the total score of transition shock
were all statistically significant （P<0.05）. Conclusion: Group counseling can effectively alleviate transition shock in intern nursing
students at infectious disease hospitals, but its long-term effects weaken over time. Therefore, in clinical nursing management and
teaching practice, it is recommended to appropriately increase intervention frequency, conduct regular group counseling activities,
consolidate effects, and better support the growth of intern nursing students.

Visualized Analysis of Literature on Nursing Research of in vitro Fertilization and Embryo Transfer in#br# China Based on CiteSpace

Huang Dan, Zhang Tongrong, Dong Jinyu, Xiong Lihua, Yang Aijing, Tang Jun

2025, 10(8):  93-100.  DOI: 10. 3969 / j. issn. 2096-2266. 2025. 08. 014

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Objective: To perform a visualized analysis of research hotspots and evolutionary trends in domestic in vitro fertilization
and embryo transfer （IVF-ET） nursing using CiteSpace, and to provide references for advancing the development of IVF-ET nursing
practice. Methods: Literatures related to IVF-ET nursing in China were retrieved from China National Knowledge Infrastructure and
Web of Science, and screened based on inclusion and exclusion criteria. CiteSpace 6.4 R1 was applied to analyze keyword cooccurrence,
institutional collaboration, and burst terms in the included literatures. Results: A total of 914 literatures were included,
comprising 757 Chinese literatures and 157 English literatures. The number of publications on IVF-ET nursing research showed an
overall annual upward trend. Research hotspots in both Chinese and English literatures mainly focus on psychological intervention,
health education, self-management, and the application of assisted reproductive technologies. Additionally, institutional collaboration
analysis revealed that relevant studies are mostly conducted through collaborations between large general hospitals and research
institutions, but inter-regional collaboration requires further enhancement. Conclusion: Research hotspots in China's IVF-ET nursing
field focus on psychological intervention, health education, and improvement of patients' self-management abilities. Meanwhile, the
application of new technologies such as artificial intelligence offers a new direction for nursing practice. However, the interinstitutional
collaboration network remains relatively loose. Future efforts should strengthen cross-regional and interdisciplinary
collaborative research to advance innovation and development in IVF-ET nursing, thereby providing more personalized and highquality
nursing services to patients.