Abstract:

Objective: To investigate the effect of allicin on proliferation of human lung adenocarcinoma cell line （SLC-89）.Methods: SLC-89 cells were cultured under different doses of allicin for 72 h, the 50 % inhibitory concentration （IC50）was measured by MTT method. SLC-89 cells were then cultured with allicin at dose IC50, IC50/2, IC50/4 for 72 h, respectively, telomerase activities of the treated cells were measured by Telomeric Repeat Amplification Protocol with ELISA（TRAP-ELISA）, and expression of proliferating cell nuclear antigen（PCNA）of the treated cells were tested by flow cytometry. Results: Allicin inhibited proliferation of SLC-89 with IC50 of 79.8 μg/mL. Allicin at dose of IC50 and IC50/2 significantly inhibited telomerase activity of SLC-89 cells, and Allicin at dose of IC50, IC50/2 and IC50/4 significantly inhibited the expression of PCNA of SLC-89 cells. Conclusion: Allicin could
inhibit proliferation of SLC-89 cells, compared to the telomerase activity, the expression of PCNA could be down-regulated more easily than by Allicin.

Key words: Allicin, telomerase activity, PCNA, SLC-89 cell, lung adenocarcinoma