J4 ›› 2011, Vol. 10 ›› Issue (12): 21-23.

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Determination of Triterpenoid in Incarvillea Mairei by Ultraviolet Spectrophotometry

Objective: To establish a method for measurement of total triterpene in Jirousheng (Incarvillea mairei) by Ultraviolet spectrophotometry (UV). Methods: Using oleanolic acid as object, and 5% vanillin-glacial acetic acid solution and perchloric acid as color-developing agent, total triterpenes was measured by spectrophotometry at 480 nm wavelength. Results: The oleanolic acid content showed a good linear relationship with absorbance (r=0.999 9) in the range of 0.04-0.24 mg/mL, and the regression equation was A =0.001 5 C+0.002 3. The average recovery was 99.37% and RSD was 1.075%(n=6), Incarvillea mairei contained 1.41% of total triterpene according to the calculation of oleanolic acid content. Conclusion: The method was simple, rapid, high sensitivity, reproducible and reliable for the quality control of Incarvillea mairei.   

  1. School of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China
  • Received:2011-06-01 Revised:2011-07-21 Online:2011-12-15 Published:2011-12-15

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Abstract:

Objective: To establish a method for measurement of total triterpene in Jirousheng (Incarvillea mairei) by Ultraviolet spectrophotometry (UV). Methods: Using oleanolic acid as object, and 5% vanillin-glacial acetic acid solution and perchloric acid as color-developing agent, total triterpenes was measured by spectrophotometry at 480 nm wavelength. Results: The oleanolic acid content showed a good linear relationship with absorbance (r=0.999 9) in the range of 0.04-0.24 mg/mL, and the regression equation was A =0.001 5 C+0.002 3. The average recovery was 99.37% and RSD was 1.075%(n=6), Incarvillea mairei contained 1.41% of total triterpene according to the calculation of oleanolic acid content. Conclusion: The method was simple, rapid, high sensitivity, reproducible and reliable for the quality control of Incarvillea mairei.

Key words: Incarvillea mairei, total triterpene, ultraviolet spectrophotometry, quality control

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