Abstract:

Objective：To investigate the rates of bacterial contamination of laboratory sheets and relevant disinfection methods.
Method：Laboratory sheets were sampled directly and bacteria were cultured in medium，and then laboratory sheets were disinfected by using different sterile techniques including ultraviolet sterilization，formaldehyde fumigation sterilization，glutaric dialdehyde fumigation method and microwave. Finally，those laboratory sheets were sampled directly again and bacteria were cultured again. Results：Two hundred and ninety-two strains bacteria grew on culture medium before ultraviolet sterilization and 146 strains after；the sterilization rate was 50%. There were 121 strains bacteria before formaldehyde fumigation sterilization and 76 strains after；the sterilization rate was 37.19%. There were 125 strains before glutaric dialdehyde fumigation sterilization and 60 strains after；the sterilization rate was 52%. There were 550 strains bacteria before Microwave sterilization and 189 strains after；the sterilization rate was 65.64%. Conclusion：Microwave sterilization showed better sterilization rate in laboratory sheets sterilization.

Key words: laboratory sheets, bacterial contamination, sterilization method