Abstract:

Objective: To compare different retrieval methods of Swine liver HBsAg / HBcAg immunohistochemical results. Methods: 15 cases of HBV seropositive swine liver were enrolled, unrepaired and simply heated for high pressure heating repair and rehabilitation of citric acid for immunohistochemistry was used to detect the results respectively. Results: There were 2 cases in the unrepaired group（13.2%）, 9 cases in the simple heating high-pressure group （60%）, 12 cases in the citric acid heated highpressure group（80%）. The results of various groups were of statistically significance,（P<0.01），and the same was true of the results of the repair group and non-repair group, But the group of simple repair and repair of citric acid were of no significant difference（P>0.05）. Conclusion: Application of antigen retrieval can get a better epitope exposure of swine liver HBsAg/HBcAg, under simply heated high pressure.

Key words: antigen, epitope retrieval, immunohistochemistry