Abstract:

Objective：To optimize the extraction process of the pinecone of protocatechuic acid in Pinus yunnanensis. Methods：
Different solvents were used to extract protocatechuic acid in Pinus yunnanensis. Their extraction efficiency was taking as index；content was determined by using HPLC chromatography. Finally，L₉（3⁴）orthogonal design experiment was used to determine the optimum the extraction process. Results：The optimum extraction process：with ethyl acetate as the extraction solvent，0.1 mol/L HCl 3 mL was added into it. Material-liquid ratio was 1∶40（mg/mL）；ultrasonic time 90 min；ultrasonic temperature 40 °C. Conclusion： The Optimized extraction process is simple，stable and have high extraction rate.

Key words: Pinus yunnanensis Faranch, pinecone, protocatechuic acid, orthogonal test