Journal of Dali University

Chemical Constituents from Underground Part of Isodon lophanthoides

SUN Jun-Zhe, CHENG Xia, ZHAO Ming-Zao, JIANG Bei

2014, 13(4): 1-3.

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Objective：To explore the chemical constituents of tuber of Isodon lophanthoides. Methods：Compounds were separated and purified by silica gel column chromatography and their structures were elucidated on the basis of spectral analysis and chemical evidences. Results：Six constituents were isolated from ethyl acetate fraction of the extract and identified as β-sitosterol（1），3β-Oacetyloleanolic acid （2），3β-O-acetylursolic acid （3），oleic acid （4），hinokiol （5），and 2 -hydroxyloleanolic acid（6）. Conclusion：Compounds 1-6 are isolated from the underground part of I. lophanthoides for the first time，while 2-6 are firstly obtained from this plant as well.

Extraction Process of Protocatechuic Acid in the Pinecone of Pinus yunnanensis

TUN Dao-Xun, LIU Lu, WANG Ying, LIU Xi, ZHANG Hai-Zhu

2014, 13(4): 4-6.

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Objective：To optimize the extraction process of the pinecone of protocatechuic acid in Pinus yunnanensis. Methods：
Different solvents were used to extract protocatechuic acid in Pinus yunnanensis. Their extraction efficiency was taking as index；content was determined by using HPLC chromatography. Finally，L₉（3⁴）orthogonal design experiment was used to determine the optimum the extraction process. Results：The optimum extraction process：with ethyl acetate as the extraction solvent，0.1 mol/L HCl 3 mL was added into it. Material-liquid ratio was 1∶40（mg/mL）；ultrasonic time 90 min；ultrasonic temperature 40 °C. Conclusion： The Optimized extraction process is simple，stable and have high extraction rate.

Analysis of Cold Medicines and Rational Drug Prescription

KONG Yan-E, FANG Hui-Xiang

2014, 13(4): 7-10.

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Objective：To investigate the medical workers how to correctly guide the patients to choose the cold medicines，and
promote the clinical rational use of cold medicines. Methods：The prescription composition of cold medicines currently on the market was analyzed；pharmacological effects of each component and adverse reactions were introduced by referring to the relevant books，documents and description，and combining with the actual work experience. Results：Different symptoms should choose different kinds of cold medicines. Conclusion：In order to achieve the purpose of safe，effective，economic and rational use of cold medicines，people should measured accurately choose cold medicines according to cold symptoms，age and pathological status.

Pharmacognostic Identification Studies on Polygonatum kingianum Coll.et Hemsl and Smilacina japonica A. Gray

HAO Jun-Jie, HAN Yun

2014, 13(4): 11-13.

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Objective：To identify Polygonatum kingianum Coll.et Hemsl and Smilacina japonica A. Gray. Methods：Macroscopic
identification method and microscopic identification method were used in the study. Results：The plant aooerance traits，their microstructure，thus and powder identification of a Polygonatum kingianum Coll.et Hemsl and Smilacina japonica A. Gray were different. Conclusion：The differences between Polygonatum kingianum Coll.et Hemsl and Smilacina japonica A. Gray，which were carried out from morphology and microscopic identification，can be used for two kinds of plant pharmacognostic identification basis.

Analysis of Growth Curve and Generation Time of Oral Candida albicans from HIV-infected Individuals

WANG Xiao-Li, WANG Cong, WANG Tao- , LIU Qi- , GUO Li-Jun- , BAI Li

2014, 13(4): 14-18.

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Objective：To realize the growth rule of oral Candida albicans from HIV-infected individuals so as to provide a theory
basis for the mechanism research of oral Candidasis，diagnosis，prevention and treatment of the disease. Methods：108 of activated oral Candida albicans from HIV-infected individuals and healthy population were grown at 37 °C for 15 hours in YPD liquid medium，measuring the number of living bacterium cells and the OD₆₀₀ at 1 h intervals. Then the growth curve was made and the generation time was calculated. Results：The growth curves of the isolates showed that 0-3 h was the lag phase，and 4-10 h was the exponential phase. After 10 hour of culture the isolates' growth entered the stationary phase. Both groups of isolates showed similar growth curves in YPD medium. The generation time of 108 isolates，the isolates from HIV -infected individuals and healthy population were 1.568 hours，1.354 hours and 1.782 hours，respectively. The generation time of the isolates in HIV-infected individuals was faster 0.428 hours than in healthy population，but the difference is not significant. Conclusion：It needs to further
study the pathogenic effect of the difference in the growth rate of the isolates from HIV-infected individuals and healthy population.

Primary Study on Lactobacillus from vaginal of High-risk Females in STD

WANG Cong, BAI Li

2014, 13(4): 18-21.

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Objective：To compare the quantity and the protection function of Lactobacillus in the vagina between the high-risk
females in STD and the healthy group，and evaluate its defense capability，in order to provide a theoretical basis for implementing the prevention and treatment interventions. Methods：71 healthy women and 120 high-risk females in STD were selected. The rate of vaginal Lactobacilli and its function of producing the organic acids，hydrogen peroxide（H₂O₂），and other antimicrobial substances in vaginal secretions were detected to quantify the number and defensive ability of Lactobacilli of high risk population. Results：The positive rate of Lactobacillus，and the protection of the high-risk females in STD were lower than the healthy group. Conclusion：Susceptibility of STD has an important relationship with the damage of the micro environment of vaginal，especially the reduction of the amount and defense ability of Lactobacillus

Study on Manufacture of Integrated Visual Pathway Specimens

GAO Yan, MA Qing, SHEN Rou, LI Qian, PU Ling-Yan- , ZHU Jian-Hua

2014, 13(4): 22-24.

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Objective：To investigate the methods of manufacturing integrated visual pathway specimens，and provide specimen making ideas for study of the visual pathway and light reflex pathway. Manufacture integrated visual pathway specimens as intuitive teaching aids for experimental teaching of human anatomy. Methods：Based on the previous experience，integrated visual pathway specimens were made from the base of skull by peeling tissues progressively. Results：Two integrated visual pathway specimens have been made. The eyes，optic nerves，optic chiasm，optic tracts，lateral geniculate bodies，optic radiation and visual cortex ware showed in the specimens. Conclusion：It is difficult to make the integrated visual pathway specimens，especially to display structures of visual pathway from the top of skull. The method of passing through the base of skull is the better way to display structures of integrated visual pathway.

Expressions and Clinical-pathological Significance of TGF-β₁，Caspase-3 and WT1 in Glomerulonephritis

ZHANG Tao, ZHANG Yu, LI Xiao-Li, HE Min-Hua

2014, 13(4): 25-29.

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Objective：To explore the expression and significance of transforming growth factor-β1（TGF-β1），cysteinyl aspartate specific proteinase -3 （Caspase -3）and Wilms' tumor suppressor gene 1 （WT1）in renal biopsy tissues of glomerulonephritis.Methods：40 cases of renal biopsy tissues and 7 cases of control renal tissues were detected and analyzed by Envision immunohistochemistry. Results：TGF -β1，Caspase -3 and WT1 expressed in all cases of renal tissues. There were significant differences between each trail grouping and normal control group （P<0.05）. The positive retes of TGF-β1 in group of mesangial proliferative glomerulonephritis were lower than membranous nephropathy（t=-4.131，P=0.002）. Expression of Caspase-3 showed no differences in divided groups. The expression of WT1 in group of massive proteinuria were lower than that non-massive proteinuria
（t =-2.130，P =0.046）. Other designed groups have no significant difference. Meanwhile，higher WT1 expression showed the correlation with lower expression of TGF-β₁ and Caspase-3. Conclusion：Up-regulated expression of TGF-β1 and Caspase-3 and down-regulated expression of WT1 were closely related to pathologic change in kidney. Proteinuria and podcytes loss is probably the predictor of renal disease progression and a cause for glomerulosclerosis.

Clinical Observation of Puerarin in Treatment of Coronary Artery Disease with Angina

WANG You-Long, LI Xue, XU Li

2014, 13(4): 30-31.

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Objective：To study the clinical effect of puerarin in treatment of coronary heart disease with angina pectoris. Methods：257 cases of coronary heart disease with angina pectoris patients were studied. They were randomly divided into observation group and control group. The control group was given conventional treatment，and the observation group was given conventional treatment and added puerarin injection. And then the effect of two groups of curative effect in patients with angina pectoris and electrocardiogram were evaluated. Results：About the total effective rate of the curative effect of angina and ECG curative effect，the observation group was significantly higher than the control group. Conclusion：Puerarin has remarkable effect on the treatment of coronary heart disease with angina pectoris.

Multiple Modes Combined Analgesia in the Application of the Artificial Knee Joint Replacement

SUN Chao-Jun, SUN Zhe, LI Hong, HU Zhi-Fu, HE Ning

2014, 13(4): 32-35.

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Objective：To compare the effect of multiple modes combined analgesia and pure postoperative analgesia in artificial
knee joint replacement. Methods：A retrospective review of 80 patients with Artificial knee joint replacement who were hospitalized from January 2009 to August 2013.They were randomly assigned to two experimental groups （n =40 per group），Multiple mode combined analgesia combined analgesia group（Group A）and pure postoperative analgesia group（Group B）. The effect of analgesia was evaluated by pain score at different time points within 72 hours after surgery and the knee joint activity. Results：The pain score of group B were higher than group A at any time point obviously（P<0.05），while the knee joint activity of group A were higher than group B（P<0.05）.Conclusion：Multiple mode combined analgesia in the application of the artificial knee joint replacement could reduce the pain of patients and could improve the knee joint early functional exercise of patients. It is safe and effective to use multiple mode combined analgesia in the application of the artificial knee joint replacement.

Clinical Observation of 100 Cases with Calcaneal Fractures Undergoing the Open Reduction and
Internal Fixation of Locking Plate

YAO Ru-Bin, YANG Kai-Shun, SHANG Xue-Hong

2014, 13(4): 36-38.

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Objective：To investigate the clinical effects of intra articular calcaneal fractures undergoing the open reduction and
internal fixation of locking plate. Methods：One hundred patients with one hundred and three fracture of calcaneus who were treated with open reduction and internal fixation of the locking plate from September 2008 to September 2011 were analyzed. There were 76 cases of male patients and 24 cases of female. Among them，97 cases were unilateral and 3 cases were bilateral. Sander type，28 cases were fractures of Sanders II，62 cases of Sanders III，10 cases of Sanders IV. All patients were treated with open reduction of L Type of lateral wall of calcaneus and internal fixation of the locking plate. Results：92 cases were followed up from 6 to 30 months （average，18 months），and the clinical results were evaluated by Maryland Scale. Excellent results were noted in 46，good in 40，fair in 4，the ratio of excellent and good results was 93.4%. Conclusion：Open reduction and internal fixation of the locking plate can be an effective method for treating the fracture of calcaneus currently.

Analysis and Treatment of 6 Cases with Tracheal Stenosis after Percutaneous Tracheotomy

LIU Hui, OU Ya-Lin

2014, 13(4): 39-40.

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Objective：To make a retrospective analysis of the reasons of tracheal stenosis after percutaneous tracheotomy.
Methods：The causes of 6 cases with tracheal stenosis from January 2008 to December 2012 in ICU after percutaneous tracheotomy and the second phase of treatment for tracheal stenosis were analyzed. Results："Ι" silicone tube of trachea dilation was carried out among the 6 cases. After 6 six months，there was no difficulty breathing and tracheal stenosis among them after their withdrawal of the tube. Conclusion：Tracheal anterolateral wall cartilage ring injury and push are the reasons of tracheal stenosis after percutaneous tracheotomy.

Comparative Study on the Treatments for Hand- foot -mouth Disease in Children with Human Recombinant Interferon alph-1b and Ribavirin Respectively

YI Jun

2014, 13(4): 41-43.

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Objective：To discuss the effect and safety of the treatment for children's hand- foot -mouth disease with human
recombinantinterferon alph-1b. Methods：388 cases of children with hand-foot -mouth disease were randomly divided into two groups which were given the same support and symptomatic treatment. Treatment group，184 cases were treated with human recombinant interferon alph-1b，< 1，1 ug（kg·d），intramuscular，2~10 10 ug/d muscle injection，1 times a day. The control group， 204 cases were treated with ribavirin〔10~15 mg /（kg·d）〕an intravenous drip. Results：The treatment group was better than control group （P<0.01）in the defervescence time，hand，foot and skin rash and mouth ulcer healing. In addition to that the individual children have a fever；there was no obvious side effect in the treatment group. Conclusion：Interferon treatment of hand，foot and mouth disease in children is safe and effective，and is worth popularizing clinically used.

Discussing the Complete Denture Restoration for the Patients with Low and Flat Alveolar Ridge

GUO Yue-Hua

2014, 13(4): 44-46.

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Objective： To design a suitable solution of the complete denture restoration by making an analysis of the traditional
methods of the complete denture restoration. Methods：Stress-free functional impression was produced. The vertical distance to the occlusal was determined exactly. Accurate tooth arrangement was planned. And the exact thickness and length of the border of the denture were measured. Use of the lingual flange was knew well. Results：The author tried to adopt new technologies while applying traditional methods to complete denture restoration and it obtained a satisfactory result. Conclusion：Design a suitable solution of the complete denture restoration by basic theories and standard operation. Notice the accuracy for each step and overcome the difficulties，which could obtain a good result for the complete denture restoration.

A Clinical Analysis of 57 Cases of Microsurgery for Lacrimal Ductile Tear

HE Ya-Ni, CHEN Yin-Chao

2014, 13(4): 47-49.

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Objective：To investigate the technique and efficacy of microsurgery for lacrimal ductile tear in order to improve the
success rate of nasolacrimal anastomosis. Methods：Surgical microscope was adopted to treat 57 cases with canaliculus disruption from January 2006 and May 2013. The microscope was used to find canalicular nasal lateral ends and the side ends of lacrimal sac. And then epidural tube was put as adhesion. Drainage tube was indwelt for 3-6 months. Follow-up was done in 3- 12 months after extubation in order to observe the curative effect. Results：53 cases were cured，and lacrimal duct flushing after extubation were unobstructed. No tears spilt. 4 cases felt that a few tears spilt，but the wash of lacrimal duct test was unobstructed，and line of lacrimal duct expansion symptoms disappeared after washing 2-3 times. No eyelid inside and outside，or the small tube or tear apart and other symptoms. Conclusion：The technique of microsurgery for lacrimal ductile tear is easier to find the tears tubular nasal side end，and surgical success rate is high. The efficacy of recovery of lacrimal duct lacrimal function is good.

Application of Sufentanil in Laparoscopic Cholecystectomy on the Elderly

FAN Zhi-Dong, YIN Guang-Fen, YANG Yi

2014, 13(4): 50-53.

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Objective：To explore the anesthetic effect of sufentanil on elderly patients with laparoscopic cholecystectomy.
Methods：One hundred and eighty elderly patients undergoing laparoscopic cholecystectomy were randomly divided into 3 groups：group F（fentanyl with dosage of 20 μg/kg），group S1（sufentanil with dosage of 0.5 μg/kg）and group S2（sufentanil with dosage of 1μg/kg）. Systolic blood pressure （SBP），diastolic blood pressure （DBP），mean blood pressure （MBP），heart rate（HR）， electrocardiogram（ECG）and pulse oxygen saturation（SpO2）at 6 time points（Respectively，10 minutes after sedation，instant of anesthesia induction，3 minsutes after endotracheal intubation，instant of carbon dioxide pneumoperitoneum，15 minutes after carbon
dioxide pneumoperitoneum and postoperative）were recorded and examined. The breathing recovery time，early wake up time and the time of air duct removal，the hemodynamic parameters of extubation，as well as the complications in 3 groups were compared. Results：Compared with group F，hemodynamics fluctuated more slightly in endotracheal intubation and around carbon dioxide pneumoperitoneum in group S1 and S2，without significant difference between S1 and S2. There was no differences in the breathing recovery time，the early wake up time and the time of air duct removal between group S1 and F. Compared with group F，hemodynamics were more stable and with less complications at air duct removal in group S1. However，spontaneous breathing recovery time extended obviously in group S2，and some patients in group S2 required additional opioid antagonist medicine which
brought fret and involuntary physical activity. Conclusion：Application of 0.5 μg/kg sufentanil in laparoscopic cholecystectomy in elderly patients could reduce the emergency responses induced by endotracheal intubation and CO2 pneumoperitoneum，maintain stable hemodynamics and would not affect the postoperative palinesthesia.

Investigation on the Bacterial Contamination of Laboratory Sheets in a Hospital of Dali

DONG Li-Wen

2014, 13(4): 54-55.

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Objective：To investigate the species of bacterial contamination of laboratory sheets. Methods：laboratory sheets were direct sampled and enrichment culture then inoculating blood Columbia agar plates and MacConkey Agar plates. Isolated bacteria were identified by morphology，Gram stain and biochemical test. Results：From 1 000 laboratory sheets，after inoculate，there were 719 bacteria growing in blood agar platein. The differential strains grew on blood Columbia agar plates and their infection rates were：Staphylococcus，518 strains，infection rates was 72.04%；Enteric bacilli，175 strains，infection rates was 24. 34%；Bacillus subtilis，26 strains，infection rates was 3.62%. The differential strains grew on MacConkey Agar plates and their infection rates were ：Staphylococcus，229 strains，infection rates was 81.03%；Enteric bacilli，70 strains，infection rates was 18.97%. Conclusion：In the inspection report，the main bacteria are mainly including Staphylococcus and Enterobacteriaceae. Staphylococcus is mainly Staphylococcus epidermidis or Staphylococcus aureus，and Enterobacteriaceae is mainly Yersinia or Shigella.

Clinical Value of Color Doppler Ultrasound and High Frequency Probing into Mesenteric Lymphadenitis of Children

WANG Zhi-Qiong, YUAN Fang, ZHENG Hong-Yun, WU Xian-Qiong, LIU Chun-Mei, ZHAO Jing, ZHU Qin-Fen

2014, 13(4): 56-58.

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Objective：To discuss the diagnostic value of color doppler ultrasound and high frequency probing into mesenteric
lymphadenitis of children，so as to provide evidence to the clinical diagnosis and treatment. Methods：A total of 141 children with acute or chronic abdominal pain were inspected by high frequency probe，and emphasis was put on peripheral umbilicus and right lower abdomen. Results：Echoes of several swollen lymph nodes with regular form and clear boundary were detected at peripheral umbilicus and right lower abdomen of all the 141 children and the structure of the hilums of the lymph nodes were clear，with aspect ratios above than 2. After 5 to 7 days' anti-infective treatment，the abdominal pains of the children relieved or disappeared. Then they were examined by color doppler ultrasound again，The results showed that the swollen lymph nodes were shrunk obviously. Conclusion：Color doppler ultrasound with high accurate rate on diagnosis of mesenteric lymphadenitis of children is the preferred
imaging examination method，which can not only help doctors to diagnose the disease timely，and also be used to observe the clinical effect and to guide the clinical medication.

The Relationship between HSP47 and Intrauterine Adhesions and Its Significance in Fibrosis Diseases

WANG Xiang-Zhen, MA Na-Na

2014, 13(4): 59-62.

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Objective：To review the significance of HSP47 in fibrosis disease and explore the pathogenesis of intrauterine
adhesions. Methods：The significance of HSP47 in fibrosis diseases such as liver fibrosis，pulmonary fibrosis，renal fibrosis and skin scar was discussed，and the relationship between HSP47 and intrauterine adhesions was explored. Results：HSP47 was high expression in fibrosis disease，and there was relationship between HSP47 and intrauterine adhesions. Conclusion：The relationship between HSP47 and intrauterine adhesion are discussed.

Analysis of the Relationship between Internet Addiction Disorder and Its Affecting Factors among Undergraduates

YANG Yi-Xing, ZHANG Tai, BAO Li-Qiong, LI You

2014, 13(4): 63-66.

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Objective：To investigate and analyze the relationship between internet addiction disorder（IAD）and its affecting factors among undergraduates in Dali University. The study aims to provide theoretical evidence for prevention，intervention and treatment of the disorder. Methods：Undergraduates who were from grade 1 to grade 4 in Dali University were surveyed by using stratified-cluster sampling method. The use of internet and general information were acquired through questionnaires of IAD. Results：The prevalence rate of IAD was 16.5% among the university students. The prevalence rate of IAD of male was higher than female. These factors which included age，sex，relationship with classmate，the aim and first time to surf the internet，lasting time of internet use were significant between IAD and No-IAD（P<0.05）. We analyzed relationship between IAD and its affecting factors among undergraduates by using Logistic regression model. We could find that the factors which included sex，first time and lasting time to surf the internet， taking part in sports were dangerous，and the factor of relationship with classmate was protective. Conclusion：The prevalence rate of IAD is high. It is a problem of public health，which affects their physical and mental health. Thus，it is necessary to take an early intervention.

Analysis of Methadone Continuous Treatment Status in Dali from 2008 to 2012

LI Hong-Yuan, WANG Qin-Li, FANG Yue-Hua , JI Shou-Lian

2014, 13(4): 67-69.

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Objective：To make an analysis of patients' status of clinical methadone continuous treatment which includes general
state，patients' number in treatment，continuous treating rate，recover reason and biological detection，in order to provide scientific basis for methadone continuous treatment work. Methods： Data on methadone continuous treatment from 2008 to 2012 were collected. Results：Biological detection rate from 2009 to 2012 were compared to show that patients in treatment had increased year by year and continuous treating rate was improved by 36.6%. Results show statistical significance （P<0.01）；recover reasons were being caught by stealthy using，diseases and inaccessibility，representing 29.7%，28.5% and 15.6%. Morphine urine test positive rate was 18.9% . Conclusion：Administration on methadone continuous treatment work should be improved. Accessibility and
converge of methadone continuous treatment work should be enlarged. Patients are provided with psychological support and social aid together with integrated interventions methods so as to increase the patients' number，maintenance rate and biological detection which will reduce the harm of drug addicts.

Epidemic Investigation of Schistosomiasis in Zhefang Town of Mangshi

DAO Bao-Xi, GUO Shu-Hong, YANG Ji-Hua, TANG Ling-Mei, ZHOU Li-Cun, DONG Jiao-Lou

2014, 13(4): 70-71.

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Objective: To know whether there is epidemic schistosomiasis in Mangshi, a non-epidemic area. Methods: 1 331
farmers in Zhefang Town of Mangshi were tested for schistosomal antibodies, and ultrasonography and rectal mucosa biopsy were performed in cases with positive results, as well as investigation of the history of epidemiology. Results: Schistosomiasis antibodies were found positive in 66 cases, with the rate of 4.96%. Actually, ultrasonography and rectal mucosa biopsy were performed in 60 cases and 16 cases were diagnosed with schistosomal hepatic injury by ultrasonography and 10 cases were diagnosed with schistosomiasis by rectal mucosa biopsy. Conclusion: None of the cases have been to epidemic areas of schistosomiasis, which shows that schistosomiasis is epidemic in Zhefang Town of Mangshi.

Analysis of the Knowledge, Attitude and Behavior of Menstruation among Female Students at a University

ZHAO Yuan, LI Yan-Nan

2014, 13(4): 72-75.

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Objective：To analyze the knowledge, attitude and behavior of menstruation among female students of a university and the influencing factors, and to provide the theoretical basis for health care in the menstrual period of female college students. Methods: A stratified random sampling was used. Questionnaires designed independently were adopted. Epidata software was used to establish database and the statistical software SPSS17.0 was chosen for statistical analysis.Results: Female college students' knowledge of menstruation mainly came from classroom learning, classmates, friends, parents, teachers, books and magazines. The difference between female students of medicine major and non-medicine major was statistically significant（P﹤0.05）as to three kinds of sources of knowledge, i.e. classroom learning（χ²=13.620, P=0.020）, books and magazines（χ²=11.684, P=0.033）, the internet（χ²=7.968, P=0.041）. The rates of awareness of menstruation between female students of medicine major and non-medicine major were 89.56% and 71.09% respectively. The difference between female students of medicine major and non-medicine major was statistically significant（P﹤0.05）as to the rates of awareness of three aspects, i.e. the concept of "menstruation"（χ²=10.263，P=0.027）, menstrual cycle （χ²=14.274，P=0.013）and menstrual period （χ²=12.827，P=0.038）. According to regression analysis, factors influencing the knowledge of menstruation included the major （medicine or non-medicine）, age, and the only child or not. The OR and 95% confidence interval were 1.980 （1.345~2.916）, 2.827 （1.382~5.785）and 0.604 （.367~.992）. Conclusion: The female college
students have some knowledge of menstruation, but it is not comprehensive. The university should strengthen education aiming at female students of different majors.

Smoking Cessation Methods of Patients with Chronic Respiratory Diseases

ZHAO Gui-Feng, DONG Jiao- , YANG Li-Fang, LI Zhan-Qiong- , ZHAO Yuan

2014, 13(4): 76-77.

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Objective: To investigate the chronic respiratory system disease patients quit rate method. Methods: 60 smokers with
chronic respiratory system disease were randomly selected from Dali University Affiliated Hospital inpatients from September to December, 2012. With stratified random sampling method, 30 cases （intervention group）were as control group and 30 cases as intervention group. Intervention group received behavioral interventions in the system, while the control system did not receive it. Two groups of subjects were the status of smoking for one year following up. Results：intervention group quit rate was 63.3%（19/30）;control group quit rate was 36.7%（11/30）; the difference has statistical meaning, P<0.05. Conclusion: Intervention group quit rate is higher than the control group with statistical significance. Prochaska proposed theoretical model of behavior change in stages,telephone follow-up, exercise guidance, and psychological intervention, which may be the references or effective measures to improve
the success rate of smoking cessation in patients with chronic respiratory diseases.

Perioperative Period Nursing on 21 Cases with Abdominal Aortic Aneurysm

KONG Gui-Fen, HU Ou

2014, 13(4): 78-80.

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Objective：To introduce the perioperative period nursing on abdominal aortic aneurysm. Methods：Before operation，the points of nursing included psycho - nursing，pain nursing，vital signs observation and blood pressure control. After operation，the focuses of nursing were basic nursing，vital signs maintain，body position nursing，respiratory tract nursing and personalized health education in perioperative period. Results：2 cases died 19 cases left hospital with no nursing complication. Conclusion：The key points to improving success rates of the operation and promoting patients' recovery are to evaluate patients correctly，carry on personalized health education and handle nursing complication in advance.

An Exploration of Applying Effective Teaching Strategies in Zoology Teaching in Normal Schools

GAO Pei-Ren, ZHANG Li-Ping

2014, 13(4): 81-84.

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On the basis of analyzing the main problems existing in the present zoology teaching in normal schools, this paper
proposes effective teaching strategies, i.e. increasing students' motivation to achieve an effective learning, developing a good teacherstudent relationship to achieve an effective teaching, establishing knowledge framework and concept maps to improve learning efficiency, raising students' problem consciousness, developing experiential learning and applying case-based teaching method.

Exploration of the Joint Instruction by Subject Teachers and English Teachers under Bilingual Instruction Environment

YIN Man-Fen

2014, 13(4): 85-89.

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Bilingual instruction is an effective way to develop multi-capability talents with the ability to perform international
communication and to realize internationalization of higher education. However，shortage of bilingual teachers has become the bottleneck of the development of bilingual instruction. This essay explores the practice of joint instruction by teachers of medical subjects and teachers of English under bilingual instruction environment. Since the advantages of both teachers of medical subjects and teachers of English are brought into full play，this joint instruction has proved to be fruitful in teachers' development in bilingual instruction and students' achievements in both learning the subjects and their English proficiency.

Reflection on Teaching and Countermeasures of the Warfield Rescue Course

YAN Wen-Jing, ZHAO Yan-Long- , TANG Jun- , DONG Wen-Ying- , WANG Yan-Shu

2014, 13(4): 90-92.

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