Analysis of Growth Curve and Generation Time of Oral Candida albicans from HIV-infected Individuals

Abstract

Abstract:

Objective：To realize the growth rule of oral Candida albicans from HIV-infected individuals so as to provide a theory
basis for the mechanism research of oral Candidasis，diagnosis，prevention and treatment of the disease. Methods：108 of activated oral Candida albicans from HIV-infected individuals and healthy population were grown at 37 °C for 15 hours in YPD liquid medium，measuring the number of living bacterium cells and the OD₆₀₀ at 1 h intervals. Then the growth curve was made and the generation time was calculated. Results：The growth curves of the isolates showed that 0-3 h was the lag phase，and 4-10 h was the exponential phase. After 10 hour of culture the isolates' growth entered the stationary phase. Both groups of isolates showed similar growth curves in YPD medium. The generation time of 108 isolates，the isolates from HIV -infected individuals and healthy population were 1.568 hours，1.354 hours and 1.782 hours，respectively. The generation time of the isolates in HIV-infected individuals was faster 0.428 hours than in healthy population，but the difference is not significant. Conclusion：It needs to further
study the pathogenic effect of the difference in the growth rate of the isolates from HIV-infected individuals and healthy population.

Key words: Candida albicans, growth curve, generation time, HIV infection

CLC Number:

R285.5

WANG Xiao-Li, WANG Cong, WANG Tao- , LIU Qi- , GUO Li-Jun- , BAI Li. Analysis of Growth Curve and Generation Time of Oral Candida albicans from HIV-infected Individuals[J]. J4, 2014, 13(4): 14-18.

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